The Rhizosphere Microbial Community of Tobacco and Its Relationship With The Properties of Leaves and Rhizosphere Soils

BackgroundRhizosphere microbes possess important effects on plant growth and quality. Here we collected tobacco roots and leaf samples from ten places in Yunnan province to investigate the interaction of the rhizosphere microbes, the soil physicochemical characteristics, and the tobacco leaf properties. ResultsA high-throughput sequencing method was used to sequence the V3–V4 region of 16S rRNA genes, and the operational taxonomic units (OTUs) were clustered using QIIME under 97% identity. A total of 4571 OTUs were obtained from the 30 tobacco root samples, and the top three phyla were Proteobacteria, Acidobacteria, and Actinobacteria, while the top three annotated genera were Gp6, Gemmatimonas, and Gp4. Redundancy analysis (RDA) showed that most of the soil physicochemical properties (10 out of 17) had a significant influence on the rhizosphere microbial community. Both correlation analysis and RDA analysis revealed that quick potassium (K) and Acidobacteria_Gp3 had a significant correlation with the tobacco leaf properties. The variance partitioning analysis showed that rhizosphere microbes had a bigger influence on the tobacco leaf properties. ConclusionsOur results showed great differences in the rhizosphere microbial diversity of tobacco and complex interaction among the microbial diversity, soil physicochemical characteristics, and tobacco leaf properties.

Evaluation of Leaf Properties of Eight Cherry Cultivars Grafted onto Maxma 14 Rootstock

The study was conducted in order to assess the leaf properties of different sweet cherry varieties grafted onto MaxMa 14 rootstock. The experiment was arranged in a randomized plot desing with three replicates of five plants per replication. The soil analyses showed that the soil properties of experiment site were found as moderate alkaline, low amount of organic material, N, P, Zn and Mn. The soil characteristics may be sub-optimum for cherry growing. According to our results, Kordia had the highest SPAD value (37.86) among the cultivars. Samba, Regina, Kordia and 0900 Ziraat cultivars had the highest anthocyanin contents. The highest stomatal conductance was in 0900 Ziraat cv followed by Sweetheart. Samba had greater leaf area (53.25 cm2) compared to other cultivars. The highest LRWC was obtained with Lorry Bloom. Epicuticular was content of Regina leaves was higher among the cultivars. The results of the present study provide useful informations about how the cherry scions grafted onto MaxMa 14 response to environmental challenges in nature. The identified leaf properties are valuable targets for physiological studies related with environmental stresses.

Particles Matters Accumulation and Anatomical Leaf Properties of Three Tree Species Growing in the Industrial Area in Jeddah, Saudi Arabia

Particles matters accumulation and anatomical leaf properties of Camphor (Cinnamomum camphora), Henna (Lawsonia inermis), and Bougainvillea (Bougainvillea spectabilis) trees growing in the industrial zone in Jeddah - Saudi Arabia and Hada Al-Shame area (control) was done. The leaf properties of all tree species growing in the industrial and control showed that each stoma had a raised edge over the guard cell region. The guard cells appeared more shrunken on the polluted leaves as compared with unpolluted leave. The results indicated that the most deposition particles on leaf surfaces of all tree species were: soot (C) and soil dust with characteristic matrix elements (Si, Al, Mg, Ca, K); fuel oil particles rich in Al, Si, Ca, and Pb; coal ash particles containing C, Al, Si, K, Ca, S; and Pb. As a result, leaves of those plant species may be used as bio-indicators for the assessment of particular matters in the industrial areas.