Abstract. Turkey is a country located in Middle Latitude zone and in which tectonic activity is intensive. Lastly, an earthquake of magnitude 6.5Mw occurred at Aegean Sea offshore on date 24 May 2014 at 12:25 UTC and it lasted approximately 40 s. The said earthquake was felt also in Greece, Romania and Bulgaria in addition to Turkey. In recent years seismic origin ionospheric anomaly detection studies have been done with TEC (Total Electron Contents) generated from GNSS (Global Navigation Satellite System) signals and the findings obtained have been revealed. In this study, TEC and positional variations have been examined seperately regarding the earthquake which occurred in the Aegean Sea. Then The correlation of the said ionospheric variation with the positional variation has been investigated. For this purpose, total fifteen stations have been used among which the data of four numbers of CORS-TR stations in the seismic zone (AYVL, CANA, IPSA, YENC) and IGS and EUREF stations are used. The ionospheric and positional variations of AYVL, CANA, IPSA and YENC stations have been examined by Bernese 5.0v software. When the (PPP-TEC) values produced as result of the analysis are examined, it has been understood that in the four stations located in Turkey, three days before the earthquake at 08:00 and 10:00 UTC, the TEC values were approximately 4 TECU above the upper limit TEC value. Still in the same stations, one day before the earthquake at 06:00, 08:00 and 10:00 UTC, it is being shown that the TEC values were approximately 5 TECU below the lower limit TEC value. On the other hand, the GIM-TEC values published by the CODE center have been examined. Still in all stations, it has been observed that three days before the earthquake the TEC values in the time portions of 08:00 and 10:00 UTC were approximately 2 TECU above, one day before the earthquake at 06:00, 08:00 and 10:00 UTC, the TEC values were approximately 4 TECU below the lower limit TEC value. Again, by using the same fifteen numbers of stations, positional variation investigation before and after the earthquake has been made for AYVL, CANA, IPSA and YENC stations. As result of the analysis made, positional displacements has been seen before and after earthquake at CANA station which is the nearest station to earthquake center. It is about 10 and 3 cm before three days and one day earthquake.