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2022 ◽  
Vol 28 (2) ◽  
Author(s):  
Miranda M. Mitchell ◽  
Amanda Vicente-Santos ◽  
Bernal Rodríguez-Herrera ◽  
Eugenia Corrales-Aguilar ◽  
Thomas R. Gillespie

Author(s):  
Daniel Maximo Correa Alcantara ◽  
Gustavo Graciolli ◽  
Ronaldo Toma ◽  
Camila Silveira Souza
Keyword(s):  

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2226
Author(s):  
Janusz T. Pawęska ◽  
Petrus Jansen van Vuren ◽  
Nadia Storm ◽  
Wanda Markotter ◽  
Alan Kemp

This study aimed to determine the vector competence of bat-associated nycteribiid flies (Eucamsipoda africana) for Marburg virus (MARV) in the Egyptian Rousette Bat (ERB), Rousettus aegyptiacus. In flies fed on subcutaneously infected ERBs and tested from 3 to 43 days post infection (dpi), MARV was detected only in those that took blood during the peak of viremia, 5–7 dpi. Seroconversion did not occur in control bats in contact with MARV-infected bats infested with bat flies up to 43 days post exposure. In flies inoculated intra-coelomically with MARV and tested on days 0–29 post inoculation, only those assayed on day 0 and day 7 after inoculation were positive by q-RT-PCR, but the virus concentration was consistent with that of the inoculum. Bats remained MARV-seronegative up to 38 days after infestation and exposure to inoculated flies. The first filial generation pupae and flies collected at different times during the experiments were all negative by q-RT-PCR. Of 1693 nycteribiid flies collected from a wild ERB colony in Mahune Cave, South Africa where the enzootic transmission of MARV occurs, only one (0.06%) tested positive for the presence of MARV RNA. Our findings seem to demonstrate that bat flies do not play a significant role in the transmission and enzootic maintenance of MARV. However, ERBs eat nycteribiid flies; thus, the mechanical transmission of the virus through the exposure of damaged mucous membranes and/or skin to flies engorged with contaminated blood cannot be ruled out.


2021 ◽  
Author(s):  
Pejić Brankaa ◽  
Budinski Ivana ◽  
van Schaik Jaap ◽  
Blagojević Jelena

Abstract Schreiber’s bent-winged bat Miniopterus schreibersii and the greater horseshoe bat Rhinolophus ferrumequinum are widespread and common cavernicolous species across southern Europe that host numerous specialized ectoparasite species. The objective of this study was to characterize the species assemblage, genetic diversity and host specificity of bat flies (Nycteribiidae, Diptera) and wing mites (Spinturnicidae, Acari) found on these bat hosts in Serbia and Bosnia and Herzegovina. Notably, while bat flies lay puparia on the cave walls and can thus be transmitted indirectly, wing mites require direct body contact for transmission. Morphological identification and sequencing of a 710-bp fragment of cytochrome oxidase I gene of 207 bat flies yielded four species, three on M. schreibersii and one on R. ferrumequinum. Sequencing of a 460-bp small subunit ribosomal RNA fragment, in all 190 collected wing mites revealed two species, one per host. In no case was a parasite associated with one host found on the other host. Species and genetic diversity of flies were higher in M. schreibersii, likely reflecting their host’s larger colony sizes and migratory potential. Mite species of both hosts showed similarly low diversity, likely due to their faster life history and lower winter survival. Our findings highlight a remarkably high host-specificity and segregation of ectoparasite species despite direct contact among their hosts in the roost, suggesting a defined host preference in the investigated ectoparasite species. Furthermore, the differences in ectoparasite genetic diversity exemplify the interplay between host and parasite life histories in shaping parasite population genetic structure.


Parasitologia ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 197-209
Author(s):  
Priscila Ikeda ◽  
Jaire Marinho Torres ◽  
Ana Julia Vidal Placa ◽  
Victória Valente Califre de Mello ◽  
Elizabete Captivo Lourenço ◽  
...  

The Anaplasmataceae family (order Rickettsiales) encompasses obligately intracellular bacteria of the genera Anaplasma, Ehrlichia, and Neorickettsia. Together with Coxiella burnetii (Coxiellaceae family, order Legionellales), these bacteria represent important causative agents of diseases in humans and animals. The scarcity of studies that investigated the occurrence of these agents in bats and their associated ectoparasites, emphasizes the need to achieve a better understanding of the role of these animals in the maintenance of such bacteria. Herein, 418 samples (133 blood, 135 spleen, and 150 ectoparasites) are collected from 135 non-hematophagous bats belonging to 12 species in a periurban area of Campo Grande city, Mato Grosso do Sul state, midwestern Brazil. In the results, 1.65% (7/418), 12.04% (50/418), and 13.63% (57/418) of samples are positive in PCR assays for Anaplasma spp. (16S rRNA gene), Ehrlichia spp. (dsb gene), and Neorickettsia spp. (16S rRNA gene), respectively. Anaplasma spp. and Neorickettsia spp. are detected in one (5.26%) Ornithodoros hasei tick larva. Ehrlichia spp. is detected in 14% of bat flies (represented by Megistopoda aranea, Trichobius costalimai, and Strebla hertigi), 6% of tick larvae (O. hasei), 12% of Spinturnicidae mites (represented by Periglischrus sp., P. torrealbai, and P. acutisternus), and 38% of Macronyssidae mites (Steatonyssuss sp.). The obtained sequences are observed to be similar to Anaplasma phagocytophilum (97.42–97.6% identified), Ehrlichia minasensis (96.73–100% identified), Neorickettsia risticii (96.7–100% identified), and Neorickettsia findlayensis (95.07–100% identified) by BLASTn analyses, and closely related to Ehrlichia ruminantium by phylogenetic analyses based on the gltA gene. No bat samples (blood/spleen) are positive in the qPCR assay for C. burnetii based on the IS1111 gene. The present work shows, for the first time, the occurrence of Anaplasmataceae in bats and associated ectoparasites (ticks, mites, and bat flies) from Brazil.


2021 ◽  
Vol 9 ◽  
Author(s):  
Daniel F. Ramalho ◽  
Ugo M. Diniz ◽  
Ludmilla M. S. Aguiar

Increasing anthropization is detrimental to the natural environment and the quality of life, affecting populations, communities, and the relationships between organisms. One of the most unique relationships in the animal world is parasitism, which often involves tightly specialized interactions between pairs of species. Bat flies, for example, are obligate ectoparasites represented by two highly adapted dipteran families that usually parasite a single bat species or genus. Recent studies have shown that bat flies could carry pathogens such as bacteria and viruses, transmitting them among bat individuals in a colony. Because host roost characteristics can influence bat-fly parasitism, we aimed to assess whether the ecological networks between parasites and their host bats are influenced by the degree of habitat anthropization. Our hypothesis was that bat-fly interaction networks would be less specialized and more nested in highly anthropized sites. We collected bat fly individuals from bats captured at 21 sampling sites located in the Federal District of Brazil and quantified the amount of natural and anthropized area within a 3-km buffer from the sampling site. Areas consisting of agriculture, construction, mining, roads, or any man-made structure were considered anthropized. Sites presented different degrees of anthropization, with areas ranging from 100% anthropized to areas retaining full natural cover. We built bat-bat fly networks for each of the sites and excluded those with less than 0.7% of sampling completeness. We calculated key weighted structural metrics for each network, such as nestedness, specialization, and modularity. The effect of the reduction in natural cover on structural metrics was assessed through GLMMs, controlling for network size and ectoparasite diversity. Nestedness increased with the amount of anthropization, while specialization and modularity did not change and were overall high in all networks. This result suggests that anthropization may influence the assembly of bat-bat fly networks, leading to the emergence of a hierarchical assembly of interactions as parasites become less specialized and interact with a wider variety of hosts. Less specialized relationships could influence parasite fitness or even increase the likelihood of transmitting pathogens between populations of different bat species.


2021 ◽  
Author(s):  
Ziqian Xu ◽  
Yun Feng ◽  
Xinxin Chen ◽  
Mang Shi ◽  
Shihong Fu ◽  
...  

Bats are reservoirs of important zoonotic viruses like Nipah and SARS viruses. However, whether the blood-sucking arthropods on the body surface of bats also carry these viruses, and the relationship between viruses carried by the blood-sucking arthropods and viruses carried by bats, have not been reported. This study collected 686 blood-sucking arthropods on the body surface of bats from Yunnan Province, China between 2012 and 2015, and they included wingless bat flies, bat flies, ticks, mites, and fleas. The viruses carried by these arthropods were analyzed using meta-transcriptomic approach, and 144 highly diverse positive-sense single-stranded RNA, negative-sense single-stranded RNA, and double-stranded RNA viruses were found, of which 138 were potentially new viruses. These viruses were classified into 14 different virus families or orders, including Bunyavirales , Mononegavirales , Reoviridae , and Picornavirales . Further analyses found that Bunyavirales were the most abundant virus group (84% of total virus RNA) in ticks, whereas narnaviruses were the most abundant (52-92%) in the bat flies and wingless bat flies libraries, followed by solemoviruses (1-29%) and reoviruses (0-43%). These viruses were highly structured based on the arthropod types. It is worth noting that no bat-borne zoonotic viruses were found in the virome of bat-infesting arthropod, seemly not supporting that bat surface arthropods are vectors of zoonotic viruses carried by bats. IMPORTANCE Bats are reservoir of many important viral pathogens. To evaluate whether bat-parasitic blood-sucking arthropods participate in the circulation of these important viruses, it is necessary to conduct unbiased virome studies on these arthropods. We evaluated five types of blood-sucking parasitic arthropods on the surface of bats in Yunnan, China and identified a variety of viruses, some of which had high prevalence and abundance level, although there is limited overlap in virome between distant arthropods. While most of the virome discovered here are potentially arthropod-specific viruses, we identified three possible arboviruses, including one orthobunyavirus and two vesiculoviruses (family Rhabdoviridae ), suggesting bat-parasitic arthropods carry viruses with risk of spillage, which warrants further study.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Haeseung Lee ◽  
Min-Goo Seo ◽  
Seung-Hun Lee ◽  
Jae-Ku Oem ◽  
Seon-Hee Kim ◽  
...  

Abstract Background Bats are hosts for many ectoparasites and act as reservoirs for several infectious agents, some of which exhibit zoonotic potential. Here, species of bats and bat flies were identified and screened for microorganisms that could be mediated by bat flies. Methods Bat species were identified on the basis of their morphological characteristics. Bat flies associated with bat species were initially morphologically identified and further identified at the genus level by analyzing the cytochrome c oxidase subunit I gene. Different vector-borne pathogens and endosymbionts were screened using PCR to assess all possible relationships among bats, parasitic bat flies, and their associated organisms. Results Seventy-four bat flies were collected from 198 bats; 66 of these belonged to Nycteribiidae and eight to Streblidae families. All Streblidae bat flies were hosted by Rhinolophus ferrumequinum, known as the most common Korean bat. Among the 74 tested bat flies, PCR and nucleotide sequencing data showed that 35 (47.3%) and 20 (27.0%) carried Wolbachia and Bartonella bacteria, respectively, whereas tests for Anaplasma, Borrelia, Hepatozoon, Babesia, Theileria, and Coxiella were negative. Phylogenetic analysis revealed that Wolbachia endosymbionts belonged to two different supergroups, A and F. One sequence of Bartonella was identical to that of Bartonella isolated from Taiwanese bats. Conclusions The vectorial role of bat flies should be checked by testing the same pathogen and bacterial organisms by collecting blood from host bats. This study is of great interest in the fields of disease ecology and public health owing to the bats’ potential to transmit pathogens to humans and/or livestock. Graphical abstract


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