Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification

The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato MTP family members for the first time, the specific and conservative StMPTs were discovered by using systematic and comparative omics. To be surprised, members of the StMTP family seem to have mutated before the evolution of dicotyledon and monocotyledon, and even the loss of the entire subfamily (subfamily G6, G7). Interestingly, StMTP9 represents the conserved structure of the entire subfamily involved in toxic metal regulation. However, the gene structure and transmembrane domain of StMTP8 have undergone specific evolution, showing that the transmembrane domain (Motif13) located at the NH2 terminal has been replaced by the signal peptide domain, so it was selected as the control gene of StMTP9. Through real-time fluorescence quantitative analysis of StMTPs under Cd and Zn stress, a co-expression network was constructed, and it was found that StMTP9 responded significantly to Cd stress, while StMTP8 did the opposite. What excites us is that by introducing StMTPs 8/9 into the ∆ycf1 yeast cadmium-sensitive mutant strain, the functional complementation experiment proved that StMTPs 8/9 can restore Cd tolerance. In particular, StMTP9 can greatly reduce the cadmium content in yeast cells, while StMTP8 cannot. These findings provide a reference for further research on the molecular mechanism of potato toxic metal accumulation.

Brain–Barrier Regulation, Metal (Cu, Fe) Dyshomeostasis, and Neurodegenerative Disorders in Man and Animals

The neurodegenerative diseases (Alzheimers, Parkinsons, amyotrophic lateral sclerosis, Huntingtons) and the prion disorders, have in common a dysregulation of metalloprotein chemistry involving redox metals (Cu, Fe, Mn). The consequent oxidative stress is associated with protein plaques and neuronal cell death. An equilibrium exists between the functional requirement of the brain for Cu and Fe and their destructive potential with the production of reactive oxygen species. The importance of the brain barrier is highlighted in regulating the import of these metals. Upregulation of key transporters occurs in fetal and neonatal life when brain metal requirement is high, and is downregulated in adult life when need is minimal. North Ronaldsay sheep are introduced as an animal model in which a neonatal mode of CTR1 upregulation persists into adulthood and leads to the premise that metal regulation may return to this default setting in ageing, with implications for the neurodegenerative diseases.

Investigation of Nutrient Elements in Cucurbita pepo Using Atomic Absorption Spectrometry

There are a number of factors to keep our health in good condition. One of them is the role of the proper amount of nutrient metals which play the important role in many reactions in the body and it becomes increasingly evident that keeping a balance level of nutrient metals in every organ is very important for maintaining a healthy body, although metals comprise only a fraction of total body weight (mg). They play crucial role for many body functions in the metabolic regulations of the human body including transporting oxygen, normalizing the nervous system. The present investigation is focused to find out the different nutrient metals that exist in the vegetable Cucurbita pepo (squash) a source of natural nutrient metals abandance and to determine the relative concentration of each metal element. Investigation has been performed on the skin, the flesh, the seed, the leaves, the stems and the roots of squash to determine the relative levels of concentrations of various metals such as elements such as Al, Ca, Cd, Co,Cr, Fe,K, Mg, Mn, Na, Ni, Pb and Zn. The Atomic Absorption Spectrometry technique is used. The proper vegetable consumption can improve the mineral and trace metal regulation and reduce cardiovascular diseases and certain cancer risks.