Effect of a medicinal agent, possessing antioxidant and antihypoxant properties, on the state of oxygen homeostasis and lipid peroxidation under experimental pneumoconiosis in albino rats

Introduction: Pneumoconioses (Pc) are interstitial pulmonary diseases of professional genesis, caused by long-term inhalation of high dust concentrations. Pcafflict 26.6-53% of the workers, exposed to dust contamination. The major feature of Pc course is irrevocability and incurability. Purpose: The research of the effect of ethylmethyl hydroxypyridine succinate, possessing antioxidant and antihypoxant characteristics, on the oxygen metabolism, acid-alkaline state, and lipid peroxidation at the background of experimental pneumoconiosis in albino rats. Material and methods: Pneumoconiosis was simulated on 72 albino rats through endotracheal injection of black coal suspension, on our own modification of O. Yu. Nykolenko methodology. Results: Manifestations of experimental pneumo-coniosis depended on the period of dust inhalation. Development of experimental pneumoconiosis was found to result in the decreased partial oxygen pressure in the arterial blood, compensated hyperventilation-induced respiratory alkalosis, and marked increase in lipid peroxidation activity that could be seen in the blood content of malon-dialdehyde, as well as of conjugated dienes and trienes. Conclusions: Application of ethylmethylhydroxy pyridine succinate in experimental pneumoconiosis has been found to improve oxygen metabolism, acid-alkaline state, and lipid peroxidation that is revealed in normalization of the partial oxygen pressure in the arterial blood, oxygen saturation of haemoglobin, index of bases’ deficiency BE (Ваse-Ехсеss), and in 1.6-2.0 decrease in the content of malondialdehyde, as well as of conjugated dienes and trienes.

Acute hepatic effects of low-dose fumonisin B1 in rats

Adult male Wistar rats were enrolled in a study to test the acute hepatic effects of 50 mg/kg fumonisin B1 in feed for 5 days. Fumonisin B1 depressed growth and feed intake, and absolute and relative liver weight showed a significant increase. The proportions of C17:0, C18:3 n3, C22:5 n3 and C22:6 n3 fatty acids decreased in the hepatic phospholipid fraction. All proportional decreases modified the hepatocellular membrane lipids into a more rigid state. The fatty acid profile modifications were partly compensated for by endogenous glutathione (preventing the formation of conjugated dienes and trienes as initial phase lipid peroxidation indicators), while the enzymatic antioxidant defence system (glutathione peroxidase) was unaltered. In contrast, hepatic malondialdehyde, the cytotoxic product of end-phase lipid peroxidation showed a concentration increase even after 5 days of feeding. The results indicate a rather strong and rapid hepatic effect of FB1, immediately impairing membrane phospholipids, even before the enzymatic antioxidant defence is activated.

Quality Characteristics and Standards for Sunflower Oil from Arad Agricultural Area

The purpose of this study was to determine the unsaturated fatty acid compounds of studied oils, physico-chemical parameters, and the behavior of the four oil types during oxidation and heating. This is a part of a large study effectuated on Carnia sunflower hybrids, PR64H91, PR65A22 and PR64H45 obtained through cold pressing. We followed the evolution of the UV absorption correlated with the peroxide value. The peroxide value has been presented an increased value in the first 12 heating hours, but after this period of time, it has been decreased due to the high temperature instability of the early formed hydro-peroxides. The absorbance between 220-230 nm and the specific absorbance on 232 and 270 nm (characteristic of the conjugated dienes and trienes) have increased along the experiment period. The results were compared with those from regular sunflower oil.

Oxidation Changes of Vegetable Oils during Microwave Heating

Oxidation changes of different types of vegetable oils were studied during microwave heating. Samples of vegetable oils (rapeseed, sunflower, soybean and corn oil), commercially available at the market in the Czech Republic, were heated in a microwave oven. Parameters as peroxide value, conjugated dienes and trienes levels were determined in oil samples before and after heating in the period from 3 to 30 minutes.