scholarly journals Autologous Tooth Dentin Graft: A Retrospective Study in Humans

Medicina ◽  
2021 ◽  
Vol 58 (1) ◽  
pp. 56
Author(s):  
José Manuel Cervera-Maillo ◽  
David Morales-Schwarz ◽  
Hilde Morales-Melendez ◽  
Lanka Mahesh ◽  
José Luis Calvo-Guirado
Keyword(s):  
Bone Formation ◽  
Bone Defects ◽  
New Bone Formation ◽  
Small Particles ◽  
Bone Replacement ◽  
Alternative Material ◽  
Sinus Lifting ◽  
Tooth Dentin ◽  
Edentulous Patients ◽  
Split Technique

Background and Objectives: The aim of this study is to evaluate the efficacy of an autologous dentin graft, via extracted teeth that are processed into bacteria-free particulate dentin in a Smart dentin grinder and then grafted immediately into alveolus post extraction or into bone deficiencies. Materials and Methods: Ten healthy, partially edentulous patients with some teeth in the mandible were recruited in the study. After their own teeth were grinded, particulate teeth were placed in empty sockets and bone defects after teeth extractions. Furthermore, after three, six, 12 and 24 months, core samples using a 3 mm trephine were obtained. Results: At three months, the particles of grinded tooth were immersed inside a new connective tissue with a small new bone formation (16.3 ± 1.98). At six months, we observed small particles of dentin integrated in new immature bone, without inflammation in the soft tissue (41.1 ± 0.76). At twelve months, we observed a high amount of bone formation surrounding tooth particles (54.5 ± 0.24), and at twenty-four months, new bone, a big structure of bone, was observed with dentin particles (59.4 ± 1.23), statistically different when compared it with at three months. Conclusions: A particulate dentin graft should be considered as an alternative material for sockets’ preservation, split technique, and also for sinus lifting. One of the special characteristics after 24 months of evaluation was the high resorption rate and bone replacement without inflammation. This material could be considered as an acceptable biomaterial for different bone defects due to its osteoinductive and osteoconductive properties

scholarly journals Mechanistic Illustration: How Newly-Formed Blood Vessels Stopped by the Mineral Blocks of Bone Substitutes Can Be Avoided by Using Innovative Combined Therapeutics

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 952
Author(s):  
Fabien Bornert ◽  
François Clauss ◽  
Guoqiang Hua ◽  
Ysia Idoux-Gillet ◽  
Laetitia Keller ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Bone Formation ◽  
Medicinal Product ◽  
Blood Vessels ◽  
Bone Substitute ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Polymeric Component ◽  
Bone Filling

One major limitation for the vascularization of bone substitutes used for filling is the presence of mineral blocks. The newly-formed blood vessels are stopped or have to circumvent the mineral blocks, resulting in inefficient delivery of oxygen and nutrients to the implant. This leads to necrosis within the implant and to poor engraftment of the bone substitute. The aim of the present study is to provide a bone substitute currently used in the clinic with suitably guided vascularization properties. This therapeutic hybrid bone filling, containing a mineral and a polymeric component, is fortified with pro-angiogenic smart nano-therapeutics that allow the release of angiogenic molecules. Our data showed that the improved vasculature within the implant promoted new bone formation and that the newly-formed bone swapped the mineral blocks of the bone substitutes much more efficiently than in non-functionalized bone substitutes. Therefore, we demonstrated that our therapeutic bone substitute is an advanced therapeutical medicinal product, with great potential to recuperate and guide vascularization that is stopped by mineral blocks, and can improve the regeneration of critical-sized bone defects. We have also elucidated the mechanism to understand how the newly-formed vessels can no longer encounter mineral blocks and pursue their course of vasculature, giving our advanced therapeutical bone filling great potential to be used in many applications, by combining filling and nano-regenerative medicine that currently fall short because of problems related to the lack of oxygen and nutrients.

scholarly journals Maxillary Sinus Augmentation Using Ceramic Alloplastic Granules or Paste: An Experimental Study in Rabbits

2021 ◽  
Vol 9 (6) ◽  
pp. 65
Author(s):  
Michael Medeiros Costa ◽  
Daniele Botticelli ◽  
Ofer Moses ◽  
Yuki Omori ◽  
Shigeo Fujiwara ◽  
...  
Keyword(s):  
Bone Formation ◽  
Maxillary Sinus ◽  
Biological Behavior ◽  
Collagen Membrane ◽  
Alloplastic Material ◽  
New Bone Formation ◽  
Sinus Augmentation ◽  
Beta Tricalcium Phosphate ◽  
Sinus Lifting ◽  
Points Of View

Background: Due to the lack of data comparing the biological behavior of two formulations, granules and paste, of alloplastic graft from microtomographic and histomorphometric points of view, the aim of the present experiment was to compare the histomorphometric and microtomographic healing of two formulations, i.e., granules (MR sites) or paste (MR-inject sites) of an alloplastic graft composed of a combination of beta-tricalcium phosphate and hydroxyapatite used for maxillary sinus lifting. Methods: A sinus lifting procedure was carried out bilaterally in 20 rabbits, and the elevated space was filled with either paste or granules of an alloplastic material. A collagen membrane was placed on the antrostomy and the animals were euthanized after 2 or 10 weeks, 10 animals each group. Microtomographic and histological analyses were performed. Results: Higher proportions of new bone formation were found at the MR, compared to the MR-inject sites both after 2 weeks (2.65 ± 2.89% vs. 0.08 ± 0.12%; p < 0.01) and 10 weeks of healing (34.20 ± 13.86 vs. 23.28 ± 10.35%; p = 0.022). Conclusions: It was concluded that new bone formation was faster in the MR sites, compared to the MR-inject. However, a longer time of healing should be allowed to make final conclusions about the efficiency in bone formation of the paste formulation of the biomaterial used in the present study.

Bioactivity of the Two Kinds of Biodegradable Composite Membrane Containing Oriented Needle-Like Apatite

2005 ◽  
Vol 284-286 ◽  
pp. 811-814 ◽  
Author(s):  
Toshiki Itoh ◽  
Seiji Ban ◽  
T. Watanabe ◽  
Shozo Tsuruta ◽  
Takahiro Kawai ◽  
...  
Keyword(s):  
Bone Formation ◽  
Composite Membrane ◽  
Composite Membranes ◽  
Bone Defects ◽  
Thigh Muscle ◽  
Poly Lactic Acid ◽  
New Bone Formation ◽  
Biodegradable Composite ◽  
Morphogenetic Protein ◽  
Old Mice

It is well known that bone morphogenetic protein (BMP) induces bone formation and requires for carriers. Poly-lactic acid / poly-glycolic acid (PLGA) is frequently used as the carriers of BMP. We developed a biodegradable composite PLGA membrane, which was containing oriented needle-like apatite with BMP. The composite membranes were implanted into the thigh muscle pouch of 3-week-old-mice. At 3 weeks after implantation, the implanted area was observed by optical microscopy. The composite membrane containing oriented needle-like apatite with BMP induced new bone formation. It seems that this composite membrane might be a scaffold of BMP and promoting the healing of bone defects.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

Evaluation in cats of a new material for cranioplasty: a composite of plaster of Paris and hydroxylapatite

1988 ◽  
Vol 69 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Charles E. Rawlings ◽  
Robert H. Wilkins ◽  
Jacob S. Hanker ◽  
Nicholas G. Georgiade ◽  
John M. Harrelson
Keyword(s):  
Bone Formation ◽  
Frontal Sinus ◽  
Wound Dehiscence ◽  
Bone Defects ◽  
The Body ◽  
New Bone Formation ◽  
Content Type ◽  
Plaster Of Paris ◽  
New Material ◽  
Fine Print

✓ The materials ordinarily used to reconstruct bone defects in the calvaria and facial bones either are difficult to shape, are partially resorbed by the body, or are likely to become infected if used near a contaminated area such as the frontal sinus. Calcium sulfate hemihydrate (plaster of Paris) has been known for years to have excellent reparative qualities in bone defects, but ordinarily it is quickly resorbed. Consequently, a new material, a composite of a dense form of plaster of Paris and hydroxylapatite, was devised to provide nonabsorbable hydroxylapatite particles for bone to form around and within during the phase of plaster absorption. Two types of this material were evaluated in cranial defects in cats. Each of the plaster of Paris/hydroxylapatite mixtures was placed into a surgically unroofed frontal sinus and into a contralateral parietal trephine hole in a group of 32 cats. Two cats in each group succumbed to anesthesia, leaving two sets of 30 cats. During the entire follow-up period there was only one other death, with no evidence of wound infection, wound dehiscence, implant rejection, or cerebral dysfunction among the survivors. The cats in each group were sacrificed at 1, 2, 3, 5, 7, 8, 9, 10, or 12 months after operation. Following sacrifice, both the frontal and parietal defects were exposed and examined visually, histologically, and with histomorphometric analysis for new bone formation. New bone formation was present as early as 1 month after operation and continued to increase during the 12 months of the study. Based upon these osteogenic qualities, the ease of shaping the composite, and the lack of infection in the frontal sinus region, it is concluded that this substance could be a valuable new material for human cranioplasty.

Biological Reaction to Electrically Polarized Hydroxyapatite

2000 ◽  
Vol 662 ◽  
Author(s):  
Tomoko Sakai ◽  
Masato Ueshima ◽  
Sadao Morita ◽  
Satoshi Nakamura ◽  
Kimihiro Yamashita
Keyword(s):  
Bone Formation ◽  
Body Fluid ◽  
Bone Defects ◽  
New Bone Formation ◽  
In Vitro Experiment ◽  
In Vivo Experiments ◽  
Biological Reaction ◽  
Positively Charged

AbstractWe have studied the polarized hydroxyapatite (HAp) whose surface was negatively or positively charged. In this study, we assessed the interfaces in vitro and in vivo periodically. As in vitro experiment, samples were immersed in simulated body fluid for 7 days and the surface was examined by scanning electron microscope (SEM). As in vivo experiments, cortical bone defects were created on the femoral trochanters and the condyles of the six Japanese white rabbits and the samples were implanted. The rabbits were sacrificed at 1, 2 and 4 W after the operation to analyze the surfaces by the SEM and the optical microscopy. In this study, a new thick apatite layer was formed on the negatively charged surface (N-surface) after 1week immersion in SBF in vitro. Besides, significant new bone formation was found at 2 weeks after the operation on N-surface in vivo, which was earlier than positively charged or non-polarized HAp surface. From this study negatively charged HAp surface by polarization accelerated the HAp crystal growth or the new bone formation. Thus, this N-surface will be promising for earlier fixation of the prosthesis or better recovery of the bone defect.

Retracted: New bone formation in bone defects after melatonin and porcine bone grafts: experimental study in rabbits

2014 ◽  
Vol 26 (4) ◽  
pp. 399-406 ◽  
Author(s):  
José-Luis Calvo-Guirado ◽  
Gerardo Gómez-Moreno ◽  
José-Eduardo Maté-Sánchez ◽  
Laura López-Marí ◽  
Rafael Delgado-Ruiz ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Formation ◽  
Bone Grafts ◽  
Bone Defects ◽  
New Bone Formation

scholarly journals Tyrosine-derived polycarbonate membrane in treating mandibular bone defects. An experimental study

2006 ◽  
Vol 3 (10) ◽  
pp. 629-635 ◽  
Author(s):  
Antti J Asikainen ◽  
Jukka Noponen ◽  
Christian Lindqvist ◽  
Mika Pelto ◽  
Minna Kellomäki ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Bone Defects ◽  
Membrane Thickness ◽  
New Bone Formation ◽  
Polycarbonate Membrane ◽  
Good Biocompatibility ◽  
The Difference ◽  
Group 2 ◽  
Group 1

This study was designed to evaluate the suitability of a novel bioabsorbable material in treating bone defects. A poly(desaminotyrosyl-tyrosine-ethyl ester carbonate) (PDTE carbonate) membrane (thickness 0.2–0.3 mm) was implanted into the mandibular angle of 20 New Zealand White rabbits to cover a through-and-through defect (12×6 mm). In group 1, the defects were left unfilled but covered with membrane and in group 2 the defects were filled with bioactive glass mesh and covered with membrane, too. Controls were left uncovered and unfilled. The animals were followed for 6, 12, 24 and 52 weeks, respectively. The material was evaluated by qualitative analysis of histological reactions and newly formed bone. We found that PDTE carbonate elicited a modest foreign body reaction in the tissues, which was uniform throughout the study. New bone formation was seen in all samples after six weeks. Group 1 had more new bone formation until 24 weeks and after this the difference settled. Based on findings of this study it was concluded that PDTE carbonate membranes have good biocompatibility and are sufficient to enhance bone growth without additional supportive matrix.

The Potential of Antibiotic Collagen Based Biocomposites for the Treatment of Bone Defects

2013 ◽  
Vol 587 ◽  
pp. 404-411
Author(s):  
Ioan Cristescu ◽  
Lucian Marina ◽  
Daniel Vilcioiu ◽  
F. Safta ◽  
M. Istodorescu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Local Treatment ◽  
Bone Destruction ◽  
Bone Defects ◽  
New Bone Formation ◽  
Collagen Scaffolds ◽  
Local Tissue ◽  
Local Bone ◽  
Antibiotic Delivery

Antibiotic delivery systems used in the past have consisted primarily of impregnated cement beads that required routine removal once the antibiotic had eluded completely. With the development of collagen scaffolds that could be used to fill bony defects the antibiotic cold be delivered from the scaffold used to sustain local bone growth. Over the course of two years antibiotic loaded collagen scaffolds were used in the local treatment of 21patients suffering of complicated fractures including bone defects, infections or pseudoarthrosis, all of them of traumatic nature. At the time of the initial surgical debridement or at subsequent second look procedures once local tissue viability was observed the antibiotic loaded collagen scaffold was inserted in the tissue defect and never removed. Excellent results were obtained and the infection was brought under control by use of both surgical and antibiotic modalities. Bone grafting was used in 6 cases where the defects were extensive. Where there was less extensive bone destruction the scaffold was a good adjuvant in new bone formation. Use of antibiotic loaded collagen scaffolds is a reliable and effective means of local antibiotic delivery system combining both the new bone formation capacity of the scaffold to hold osteoblasts with the ability to deliver high doses of antibiotic in the local tissue environment and thus avoiding the systemic toxicity.

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