Retinal fundus photographs capture hemoglobin loss after blood donation

Recently it was shown that blood hemoglobin concentration could be predicted from retinal fundus photographs by deep learning models. However, it is unclear whether the models were quantifying current blood hemoglobin level, or estimating based on subjects' pretest probability of having anemia. Here, we conducted an observational study with 14 volunteers who donated blood at an on site blood drive held by the local blood center (ie, at which time approximately 10% of their blood was removed). When the deep learning model was applied to retinal fundus photographs taken before and after blood donation, it detected a decrease in blood hemoglobin concentration within each subject at 2-3 days after donation, suggesting that the model was quantifying subacute hemoglobin changes instead of predicting subjects' risk. Additional randomized or controlled studies can further validate this finding.

Effect of maternal anemia on cord blood haemoglobin of newborn

Background: Anemia during pregnancy is linked to major maternal and fetal problems. The aim of the study was to find any correlation between maternal anemia and neonatal cord blood haemoglobin (Hb) levels.Methods: This cross-sectional study was done in the department of pediatrics and department of obstetrics and gynaecology at the Hind Institute of Medical Sciences, Barabanki over 9 months from January 2021 to September 2021. A total of 308 pregnant females more than 34 weeks of gestation who delivered participated in the study and cord blood hemoglobin of their new-born was collected. The mean maternal hemoglobin and mean cord blood hemoglobin were compared using the Pearson correlation coefficient in different groups i.e., non-anemic, anemic, mild, moderate, and severe anemic groups. Results: Out of 308 pregnant women, 198 (64.3%) were anemic, and 110 (35.7%) were nonanemic. The mean maternal hemoglobin among non-anemic mothers and anemic mothers was 11.91 (0.70) and 9.14 (1.56) respectively. The Pearson correlation coefficient of non-anemic, anemic, mild, moderate, and severe anemic groups were 0.6985, 0.8453, 0.7772, 0.6321, and 0.7226 respectively with a statistically significant p value<0.05 showing a positive correlation between maternal hemoglobin and cord hemoglobin.Conclusions: From this study, we concluded that maternal anemia affects neonatal cord blood hemoglobin. According to the findings, anemic women deliver new-born with lower hemoglobin levels than non-anemic mothers. The findings revealed a linear correlation between maternal hemoglobin and cord blood hemoglobin of their new-born.

Angelica archangelica and Ginkgo biloba Extracts Recover Functional Blood Hemoglobin Derivatives in Rabbits Exposed to High Altitude

Background: Shortage of oxygen is a common condition for residents of high-altitude (HA) areas. In mammals, hemoglobin (Hb) has four derivatives: oxyhemoglobin (Hb-O2)‎, carboxyhemoglobin (Hb-CO), sulfhemoglobin (Hb-S), and methemoglobin (Met-Hb). In HA areas, aberrant physiological performance of blood hemoglobin is well-established. Objective: The study aimed to investigate the influence of 30 days of HA residence on rabbits' total Hb, Hb derivatives, Hb autooxidation rate, and antioxidant enzymes in comparison to low-altitude control rabbits. Further, the study aimed to investigate the effect of antioxidant-rich Angelica archangelica and/or Ginkgo biloba extracts on the same parameters in HA-resident rabbits. Methods: Rabbits subjected to 30 days of HA residence were compared to low-altitude control rabbits. HA-residence rabbits were then orally administered 0.11 g/kg b.wt. of Angelica archangelica and/or Ginkgo biloba extract for 14 days. Hb derivatives and Hb autooxidation rate were measured spectrophotometrically. Antioxidant enzymes were estimated using specialized kits. Results: Compared to low-altitude rabbits, 30-day HA-residence rabbits showed a noticeable increase (p<0.05) in Hb-O2‎ and Hb-CO concentration. In addition, Met-Hb concentration, autooxidation rate of Hb molecules, and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) exhibited a remarkable increase in HA-residence rabbits (p<0.01), reflective of rapid ROS generation. In HA-residence rabbits, both individual and combined treatment with antioxidant-rich extracts for 14 days resulted in recovery to near-normal functional levels of Hb-O2‎ and Met-Hb, Hb autooxidation rate, and activities of SOD and GPx, while only combined treatment led to Hb-O2 recovery. Conclusion: The findings suggest that functional Hb levels may be recovered by oral administration of A. archangelica, G. biloba, or combined treatments. In conclusion, oxidative stress due to living in HA areas may be avoided by supplementation with natural antioxidants.

Blood Hemoglobin Substantially Modulates the Impact of Gender, Morbid Obesity, and Hyperglycemia on COVID-19 Death Risk: A Multicenter Study in Italy and Spain

BackgroundHyperglycemia and obesity are associated with a worse prognosis in subjects with COVID-19 independently. Their interaction as well as the potential modulating effects of additional confounding factors is poorly known. Therefore, we aimed to identify and evaluate confounding factors affecting the prognostic value of obesity and hyperglycemia in relation to mortality and admission to the intensive care unit (ICU) due to COVID-19.MethodsConsecutive patients admitted in two Hospitals from Italy (Bologna and Rome) and three from Spain (Barcelona and Girona) as well as subjects from Primary Health Care centers. Mortality from COVID-19 and risk for ICU admission were evaluated using logistic regression analyses and machine learning (ML) algorithms.ResultsAs expected, among 3,065 consecutive patients, both obesity and hyperglycemia were independent predictors of ICU admission. A ML variable selection strategy confirmed these results and identified hyperglycemia, blood hemoglobin and serum bilirubin associated with increased mortality risk. In subjects with blood hemoglobin levels above the median, hyperglycemic and morbidly obese subjects had increased mortality risk than normoglycemic individuals or non-obese subjects. However, no differences were observed among individuals with hemoglobin levels below the median. This was particularly evident in men: those with severe hyperglycemia and hemoglobin concentrations above the median had 30 times increased mortality risk compared with men without hyperglycemia. Importantly, the protective effect of female sex was lost in subjects with increased hemoglobin levels.ConclusionsBlood hemoglobin substantially modulates the influence of hyperglycemia on increased mortality risk in patients with COVID-19. Monitoring hemoglobin concentrations seem of utmost importance in the clinical settings to help clinicians in the identification of patients at increased death risk.

Society of Physicians at Kazan University. Pediatric section. Meeting on June 3, 1929

1) Dr. S. M. Marcuse. On the issue of chronic anemia in schoolchildren. The report was published in No. 10 Kaz. honey. magazine ".2) Dr. EP Krever. On the pathogenesis of anemia in children. The report will be published in Kaz. honey. magazine ". 3) Dr. 3. I. Malkina. On the question of reticulo-endotheliosis. Debate: prof. Vasiliev, Lepsky, assistant professor Vorobiev, and prof. Menshikov. 4) Dr. O.S. Gershtein. "Experience of helminthological examination of children in Kazan with the data of blood hemoglobin and body weight before and after deworming." The speaker examined the pupils of 2 schools of the 1st stage (Tatar and Russian) for helminthic infestations, using the Flleborn, Teleman and scraping methods to find the eggs of the helminths. In total, 466 hours were examined. The total infection of children with worms is very significant, reaching 71.6%. There is no difference in the infection of children based on ethnicity. The greatest percentage of infection falls between the ages of 8-15 years. Of the types of worms, enterobius (87.6%) and ascaris lumbricoides are most common. Before and after treatment, the patients' blood was examined, and their weight was measured. As expected, in most children, the weight and% Hb increased after the expulsion of the worm. Debate: Dr. Rozov, assistant professor Vorobiev, prof. Lepsky and Menshikov.

Distribution of crystalloid fluid infused during onset of anesthesia-induced hypotension: a retrospective population kinetic analysis

Background Induction of anesthesia causes a drop in arterial pressure that might change the kinetics of infused crystalloid fluid. The aim of this report is to provide a mathematical view of how fluid distributes in this setting. Methods Data were retrieved from three studies where 76 patients (mean age 63 years, mean body weight 66 kg) had received approximately 1.1 L of Ringer’s solution over 60 min by intravenous infusion before and during induction of spinal, epidural, or general anesthesia. A population kinetic model was used to analyze the fluid distribution and its relationship to individual-specific factors. Frequent measurements of blood hemoglobin and the urinary excretion served as dependent variables. Results Before anesthesia induction, distribution to the extravascular space was threefold faster than elimination by urinary excretion. Both distribution and elimination of infused fluid were retarded in an exponential fashion due to the anesthesia-induced decrease in the mean arterial pressure (MAP). A decrease in MAP from 110 to 60 mmHg reduced the rate of distribution by 75% and the rate of elimination by 90%. These adaptations cause most of the infused fluid to remain in the bloodstream. Age, gender, type of anesthesia, and the use of ephedrine had no statistically significant effect on plasma volume expansion, apart from their possible influence on MAP. Conclusion The decrease in MAP that accompanies anesthesia induction depresses the blood hemoglobin concentration by inhibiting both the distribution and elimination of infused crystalloid fluid. The report provides mathematical information about the degree of these changes.

The Link Between Indoor Air Pollution From Cooking Fuels and Anemia Status Among Non-pregnant Women of Reproductive Age in Ethiopia.

The effect of indoor air pollution from different fuel types on the anemia status among non-pregnant women of reproductive ages is rarely studied. This study aimed to assess the link between indoor air pollution from different fuel types and anemia among non-pregnant women of reproductive ages in Ethiopia. The secondary data from the 2016 Ethiopian Demographic and Health Survey data have been employed for this study. The women of reproductive age who were not pregnant at the time of the data collection were the study population and their anemia status was the outcome variable with multiple outcomes as (moderate to severe, mild, and no anemia) and households using biomass fuel and clean fuel were selected for this study. Multinomial logistic regression was employed to estimate the association of biomass fuel use with the anemia status of women in reproductive age controlling for age, body mass index, education level, exposure to household tobacco smoke, type of residence, wealth index, and region. Adjusted Odds Ratio (AOR) was calculated at 95% Confidence Interval. An independent sample t-test was used to assess the mean difference in blood Hemoglobin level (g/dl) between biomass and clean fuel users. For all statistical tests, a p-value < 0.05 was considered significant. The proportion of anemia in women of reproductive age in Ethiopia was 41.8% and 19.4 % among biomass fuel and clean fuel users respectively. The mean blood hemoglobin level was 127.17(± 18.063) g/dl. In the multivariable multinomial logistic regression analysis, women of reproductive age who utilize biomass fuel for cooking were 33 % more likely to have mild anemia than households who use cleaner fuels, whereas the association of biomass fuel use with moderate to severe anemia was insignificant. When compared with non-pregnant women who use clean energy types, women who live in households that use biomass fuel have a lower (5.8 g/dl ) blood Hemoglobin level P < 0.001. The finding indicated that the use of biomass fuel was associated with reduced blood Hemoglobin levels and significantly associated with mild anemia levels in women of reproductive age in Ethiopia. Interventions that reduce or prevent indoor air pollution from biomass fuels must be implemented in Ethiopia.

Analysis of the impact of long non-coding RNA gene polymorphisms (ANRIL, MALAT1, HOTAIR) on some clinical and pathologic features in patients with bladder cancer

Objective – to analyze the possible association between the SNPs rs4977574 (ANRIL gene), rs3200401 (MALAT1 gene), rs1899663 (HOTAIR gene) and tumor size and some clinical and laboratory testing data in patients with transitional cell carcinoma of the urinary bladder (TCCUB).Material and methods. Whole venous blood of 141 patients with TCCUB was used. The genotyping of rs4977574 and rs3200401 sites was performed by real-time polymerase chain reaction (Real-time PCR). The rs1899663 locus genotyping was done by polymerase chain reaction followed by restriction fragment length polymorphism analysis (PCR-RFLP). Mathematical analysis of the obtained data was performed using the software package SPSS (version 17.0).Results. It was found that individuals with TT-genotype (rs3200401-polymorphism) have a lower blood hemoglobin content ((106.3 ± 23.9) g/l; P = 0.043) and higher blood glucose ((7.1 ± 2.3) mmol/l; P = 0.043) and creatinine ((104.5 ± 33.8) μmol/l; P = 0.022) than patients with CC genotype (respectively: (131.1 ± 21.9) g/l); (5.4 ± 1.5) mmol/l); (83.8 ± 18.5) μmol/l)). TT-homozygotes also have the higher tumor width ((4.2 ± 1.7) cm; P = 0.027) than in CC-homozygotes ((2.9 ± 1.1) cm). No significant association between rs4977574, rs1899663 loci and studied parameters was found.Conclusion. The MALAT1 gene rs3200401 polymorphism is associated with tumor size and blood hemoglobin, glucose, and creatinine levels in patients with TCCUB. No association between rs1899663, rs4977574 loci and clinical and pathological features in patients with TCUUB was detected.