The objective of this study was to determine the persistence and leaching of the herbicide oxadiazon in five substrates. The substrate mixtures consisted of the following: peatmoss, compost, and sand in the following proportions: 1:1:0, 3:3:2, 1:1:2, 1:1:6, and 0:0:1 in 5-liter containers. Rates of oxadiazon used were 4 and 8 kg a.i./ha on two separate split-split plots. Each experimental design had three factors: five substrates, four harvest times (24 h; 1, 2, and 3 months) and five soil depths (0–2, 2–4, 4–6, 6–8, 8– cm). Only herbicide persistence and leaching from the various substrates were investigated in this experiment; therefore, we did not remove plant material. Substrate oxadiazon residues were determined by gas chromatography analysis, and it was shown that leaching was more evident in media with a lower percentage of organic matter. In addition, oxadiazon did not leach below 4 cm in conventional substrate (1 peatmoss: 1 compost: 1 sand, respectively). The persistence of oxadiazon was affected by soil composition and herbicide persisted more in substrates with great percentage of organic matter.