Microstratigraphic preservation of ancient faunal and hominin DNA in Pleistocene cave sediments

Ancient DNA recovered from Pleistocene sediments represents a rich resource for the study of past hominin and environmental diversity. However, little is known about how DNA is preserved in sediments and the extent to which it may be translocated between archaeological strata. Here, we investigate DNA preservation in 47 blocks of resin-impregnated archaeological sediment collected over the last four decades for micromorphological analyses at 13 prehistoric sites in Europe, Asia, Africa, and North America and show that such blocks can preserve DNA of hominins and other mammals. Extensive microsampling of sediment blocks from Denisova Cave in the Altai Mountains reveals that the taxonomic composition of mammalian DNA differs drastically at the millimeter-scale and that DNA is concentrated in small particles, especially in fragments of bone and feces (coprolites), suggesting that these are substantial sources of DNA in sediments. Three microsamples taken in close proximity in one of the blocks yielded Neanderthal DNA from at least two male individuals closely related to Denisova 5, a Neanderthal toe bone previously recovered from the same layer. Our work indicates that DNA can remain stably localized in sediments over time and provides a means of linking genetic information to the archaeological and ecological records on a microstratigraphic scale.

Characteristics and impact of aged coal ash with slag emplaced in a karst cave: the case of Divaška jama, Slovenia

A mixture of coal bottom ash and slag, with a fraction of fly ash (CAFAS) from steam locomotives, was placed in the cave Divaška jama to delimit and level tourist trails. Emplacement began in 1914 and carried on for several decades. The CAFAS mixed with other cave material gradually changed its structure and appearance. Currently the concentration of some elements in the CAFAS (As, Cu, Hg, Ni, Pb, Zn), and also to a lesser extent in cave sediments (Cr, Cu, Ni), indicates a possibly harmful effect on sediment-associated biota based on ecotoxicological assays. Compared to the cave sediment, the CAFAS contains distinctly different mineral phases and presents a different source of radioactivity. Microbial metabolic activity of CAFAS is low, 0.22 μl O2/gDW h, but higher than that of cave sediment. The present environmental hazards from CAFAS are estimated to be low. Whereas the emplacement of CAFAS was seen initially a long-term solution for waste disposal and management of the cave, it turned out that CAFAS enriches the underground environment with inorganic and organic compounds and disperses pollution into the cave ecosystem. After its removal from the cave, the CAFAS should be investigated thoroughly due to its susceptibility to alteration.

The Effect of Formation Processes on The Frequency of Palaeolithic Cave Sites in Semi-Arid Zones: Insights From Kazakhstan

Central Asian caves with Palaeolithic deposits are few but they provide a rich record of human fossils and cultural assemblages that has been used to model Late Pleistocene hominin dispersals. However, previous research has not yet systematically evaluated the formation processes that influence the frequency of Palaeolithic cave sites in the region. To address this deficiency, we combined field survey and micromorphological analyses in the piedmont zone of south Kazakhstan. Here we present our preliminary results focusing on selected sites of the Qaratau mountains. Sediment cover varies among the surveyed caves and loess-like sediments dominate the cave sequences. The preservation of cave deposits is influenced by reworking of cave sediments within the caves but also by the broader erosional processes that shape semi-arid landscapes. Ultimately, deposits of potentially Pleistocene age are scarce. Our study provides new data in the geoarchaeologically neglected region of Central Asia and demonstrates that micromorphology has great analytical potential even within the limitations of rigorous survey projects. We outline some of the processes that influence the formation and preservation of cave deposits inKazakhstan, as well as broader implications for the distribution of Palaeolithic cave sites in Central Asia and other semi-arid environments.

Changes in Cave Sedimentation Mechanisms During the Late Quaternary: An Example From the Lower Cerovačka Cave, Croatia

During archeological excavations in the Lower Cerovačka Cave (Mt. Velebit, Croatia), the test trench penetrated to a depth of 1.8 m. An undisturbed sequence of sediments was exposed. Considering that caves represent highly efficient sediment traps it was possible to recognize changes in the depositional mechanisms during the Pleistocene–Holocene period. Using the multiproxy approach, the mineralogical, petrographic, and biostratigraphic characterization of the cave sediments was performed. Facies analysis revealed several stages in the development of the clastic filling of cave channels. Allochthonous origin of the sediment was assumed. Sedimentation took place under various conditions from pronounced cold and dry climate during Pleistocene stages in the base of the profile, to humid periods with anthropogenic influence during the Holocene at the very top of the profile. Although traditionally these sediments were believed to be of a Pleistocene age, here for the first time a stratigraphic calibration of the profile has been performed based on luminescence dating of detrital cave sediments and radiometric dating of speleothems.

Hints on the Late Miocene Evolution of the Tonale-Adamello-Brenta Region (Alps, Italy) Based on Allochtonous Sediments From Raponzolo Cave

Raponzolo is a paleo-phreatic cave explored in 2011 in the Brenta Dolomites (Trentino, Italy), at the remarkable altitude of 2,560 m a.s.l. Differently to all other caves of the area, it hosts well-cemented fine to medium sands of granitic-metamorphic composition. The composition suggests a sediment source from the Adamello and Tonale Unit, separated from the Brenta by one of the most important tectonic lineaments of the Alps (Giudicarie Line). The fine-sand sediment was sampled to determine burial time and thus a minimum age of the cave. Cosmogenic isotopes (26Al and 10Be) in quartz grains allowed to estimate a minimum burial age of 5.25 Ma based on the mean sediment transport time at the surface and infer original altitude of the catchment area. Detrital apatite fission-track (AFT) and U-Pb dating on zircons provide information on the source, both from a regional and altitude (exhumation) perspective. Two populations of detrital AFT ages center at 17 (−2.3 + 2.6) Ma and 23 (−3.3 + 3.9) Ma, whereas the main detrital zircon U-Pb age populations are younger than 40 Ma. These correspond to intrusive and metamorphic sources nowadays outcropping exclusively above 2,200–2,300 m a.s.l. in Northern Adamello and Tonale. The results point to a late Miocene erosion and infilling of the cave by allochtonous sediments, with important implications on the timing of cave speleogenesis, as well as the paleogeographical connection, tectonic evolution and uplift of different structural units of the Alps. The roundness and the well sorted size of the quartz grains suggest a fluvial or aeolian origin, possibly recycled by glacial activity related to cold events reported in high latitude areas of the world at 5.75 and 5.51 Ma. These glacial phases have never been documented before in the Alps. This information confirms that the valleys dividing these geological units were not yet deeply entrenched during the onset of the Messinian Salinity Crisis (5.6–5.5 Ma), allowing an efficient transport of sediments across major tectonic lineaments of the Alps. This study shows the potential of cave sediments to provide information not only on the age of speleogenesis but also on the paleogeography of a wide area of the Alps during the late Miocene.