Compact NMR Spectroscopy for Low-Cost Identification and Quantification of PVC Plasticizers

Polyvinyl chloride (PVC), one of the most important polymer materials nowadays, has a large variety of formulations through the addition of various plasticizers to meet the property requirements of the different fields of applications. Routine analytical methods able to identify plasticizers and quantify their amount inside a PVC product with a high analysis throughput would promote an improved understanding of their impact on the macroscopic properties and the possible health and environmental risks associated with plasticizer leaching. In this context, a new approach to identify and quantify plasticizers employed in PVC commodities using low-field NMR spectroscopy and an appropriate non-deuterated solvent is introduced. The proposed method allows a low-cost, fast, and simple identification of the different plasticizers, even in the presence of a strong solvent signal. Plasticizer concentrations below 2 mg mL−1 in solution corresponding to 3 wt% in a PVC product can be quantified in just 1 min. The reliability of the proposed method is tested by comparison with results obtained under the same experimental conditions but using deuterated solvents. Additionally, the type and content of plasticizer in plasticized PVC samples were determined following an extraction procedure. Furthermore, possible ways to further decrease the quantification limit are discussed.

A pragmatic eLCR for an ultrasensitive detection of methicillin-resistant Staphylococcus aureus in joint synovial fluid: superior to qPCR

A pragmatic electrochemical mecA gene detection method for a rapid, accurate and low-cost identification of MRSA in the joint synovial fluid of PJI patients.

Quality Cost and Economic Analysis. A Synthesis in the Manufacturing Systems

The paper performs a literature review on existing models and methodologies to analyse and identify the cost of quality in manufacturing systems. The review shows that existing accounting models are insufficient to identify quality cost in detail in production processes. Existing models also do not cover the entire production activities and therefore need to be extended over the entire product life cycle. The authors therefore develop a new approach by refining and extending the method of activity-based costing to make it applicable for quality cost identification. The proposed methodology can serve as a building block for a later integration into superior supply chain management systems which allow to trigger continuous quality improvements of entire production networks.

Architecture of a multi-channel and easy-to-make microfluidic paper-based colorimetric device (μPCD) towards selective and sensitive recognition of uric acid by AuNPs: an innovative portable tool for the rapid and low-cost identification of clinically relevant biomolecules

A portable microfluidic paper-based colorimetric sensor based on various type of gold nanoparticle (AuNPs) for the monitoring of UA was developed.

Identification of a Pangolin Niche for a 2019-nCoV-like Coronavirus via an Extensive Meta-metagenomic Search

In numerous instances, tracking the biological significance of a nucleic acid sequence can be augmented through the identification of environmental niches in which the sequence of interest is present. Many metagenomic datasets are now available, with deep sequencing of samples from diverse biological niches. While any individual metagenomic dataset can be readily queried using web-based tools, meta-searches through all such datasets are less accessible. In this brief communication, we demonstrate such a meta-meta-genomic approach, examining close matches to the Wuhan coronavirus 2019-nCoV in all high-throughput sequencing datasets in the NCBI Sequence Read Archive accessible with the keyword “virome”. In addition to the homology to bat coronaviruses observed in descriptions of the 2019-nCoV sequence (F. Wu et al. 2020, Nature, doi.org/10.1038/s41586-020-2008-3; P. Zhou et al. 2020, Nature, doi.org/10.1038/s41586-020-2012-7), we note a strong homology to numerous sequence reads in a metavirome dataset generated from the lungs of deceased Pangolins reported by Liu et al. (Viruses 11:11, 2019, http://doi.org/10.3390/v11110979). Our observations are relevant to discussions of the derivation of 2019-nCoV and illustrate the utility and limitations of meta-metagenomic search tools in effective and rapid characterization of potentially significant nucleic acid sequences.ImportanceMeta-metagenomic searches allow for high-speed, low-cost identification of potentially significant biological niches for sequences of interest.

Main challenges in the identification and measurement of indirect costs in projects: a multiple case study

Projects have direct and indirect costs; the direct ones are easily identified and quantified, the indirect ones are difficult to control, because they are generated in favor of more than one project, and even if they are not directly related to the activities, they influence their budget. This study aims to present indirect cost identification methods, analyze the estimation models adopted for its measurement, and also analyze ways of appropriating costs in several projects. The method adopted was the analysis of multiple cases in companies of the industrial, commercial and services sectors. It was verified that projects with greater detail of the scope show less difficulty in identifying indirect costs, directly influencing the method of estimation adopted. The estimate for indirect costs is specific to each company, and its complexity is defined by the volume of data available. The appropriation of indirect costs differs between cases, but has similar objectives in its incorporation in the direct hours of the projects. It is worth highlighting the importance of project planning in the identification of indirect costs, in the choice of measurement methods, and also in the forms of costing for the budget.