Structural Organization of the Liver in the Presence of Pig Hepatosis

The structural organization of the liver in hepatosis and its clinical and morphological manifestation in pigs were studied. In animals with reduced vitality, the liver underwent deep microscopic and ultrastructural changes. At hepatic steatosis, the liver had a cellular structure, many lipid vacuoles and the damage of mitochondrial membranes of a granular endoplasmic reticulum appeared in hepatocytes. Proteinosis was characterized by impaired of lobular structure, with protein swelling of the cytoplasm of hepatocytes and Disse’s spaces expansion, significant decrease of glycogen; mitochondrial swelling and the appearance of lysosomal vacuoles in the hepatocyte. In massive hepatic necrosis, hepatocyte dystrophy was noted, significant histochemical decrease of glycogen, lysis of the hepatocyte granule endoplasmic reticulum, pycnosis of the hepatocyte nucleus, and in general necrobiotic changes in the liver cells.

Morphological criteria for diagnosis of primary hyperparathyroidism

The work performed on the operating material. A morphological study of the parathyroid glands removed in 128 patients with hyperparathyroidism was performed. For the histological study pieces of the parathyroid gland were taken. The pieces were fixed in 10 % buffered formalin for 10 days, then washed in running water, passed through a processor and embedded in paraffin. From paraffin blocks, sections of 5–6 µm were prepared. Sections were stained with hematoxylin and eosin, pikrofuksin according to Van Gieson, toluidine blue, according to Mallory, used CHIC-reaction. The results of the study showed that primary hypothyroidism is more common in women (82 %) aged 41–50 years (42.2 %). The size of the parathyroid glands ranged from 0.3 to 8.2 cm. Adenomas were found in 51.6 % of patients, hyperplasia in 43.0 %, cancer in 5.4 % of them. In primary hyperplasia, there is an increase in all parathyroid glands, upper pair or lower pair. Upper pairs are increased more. Histological examination indicates lobular structure of the gland, lobular hyperplasia, diffuse proliferation of the main, acidophilic and transitional forms of parathyrocytes, monomorphic structure, reduction or absence of stromal fat, the presence of a large number of vessels. Hyperplasia of the main cells was detected in 67 % of patients, hyperplasia of acidophilic cells in 28 %, hyperplasia of light cells in 3 %, mixed hyperplasia in 2 % of them.

Heat Induced Oxidative Stress and Aberrations in Liver Function Leading to Hepatic Injury in Rats

Exposure to heat stress (HS) elicits systemic and cellular response in experimental animals and humans. The current study was undertaken to determine the effect of HS on liver microstructure and function in rats. A heat simulation chamber with ambient temperature (Ta) 45 ± 0.5 °C and relative humidity (RH) 30 ± 5 per cent was used to expose animals to HS. Rats were categorised as moderately heat stressed (MHS, Tc = 40 °C) and severely heat stressed (SHS, Tc = 42 °C) group. We observed that with rise in core temperature (Tc) alanine aminotransferase(ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were increased but glucose level was decreased in both plasma and liver tissue. Significantly elevated levels of reactive oxygen species (ROS) and nitric oxide (NO) were detected in liver of MHS and SHS animals. Additionally, glutathione disulfide and glutathione (GSSG and GSH) ratio was found to be increased with rise in Tc which suggested saturation in antioxidant capacity of tissue. Furthermore, levels of heat shock proteins (HSPs) and caspases were upregulated upon HS. Results of histological examination indicated extensive loss of cells in liver parenchyma leading to disorganisation of lobular structure. Thus, biochemical and histological studies in experimental animals demonstrates that HS may severely altered structural and biochemical functions of liver.

Study of acute hepatotoxicity of Equisetum arvense L. in rats

PURPOSE: To evaluate the acute hepatotoxicity of Equisentum arvense L. in rats. METHODS: Fifty Wistar rats were used, these being divided in four groups, one being the control (receiving only water) and the other groups receiving graded doses of Equisentum arvense L. (30, 50, and 100mg/kg respectively) for 14 days. Blood samples were obtained to determine TGO, TGP, FA, DHL and GT-gamma activities. After that, hepatic tissue samples were collected for the anatomopathologic analysis. RESULTS: The anatomopathologic exam of the hepatic tissue showed organ with preserved lobular structure. In the same way, there was no significant change in the seric activities of the hepatic enzymes when compared to control group. CONCLUSION: The oral treatment with graded doses of Equisentum arvense L. was not able to produce hepatic changes. Further studies are necessary to evaluate the chronic hepatotoxicity of Equisentum arvense L. in rats.

Some aspects of plant penetration by Myzus persicae (Sulz.) nymphs (Homoptera, Aphididae)

The feeding penetrations of Myzus persicae (Sulz.) nymphs into tulip leaf epidermis, as shown by a study of stylets and tracks, may be intercellular, intracellular or stomatal with the former predominating. Contact between the stylet sheath or track and cell cytoplasm is at a maximum during intracellular entry but occurs to a limited extent during intercellular penetration due to partial rupture of the epidermal end walls. In the mesophyll, the stylet path is intercellular but a few cells were penetrated by tracks.Tracks vary in appearance according to the degree of confinement to which they have been subjected, the differing amounts of gelling saliva involved beingattributed to alterations in salivation rate or speed of penetration of the bundle. Seventypes of track were recognised, ranging from a thin membraneous to a thick lobular structure. The tracks have recognisable precursors in the stylet sheaths though differences between sheaths and tracks are evident, particularly where saliva is secreted during stylet withdrawal. In the vascular tissues, phloem and xylem are penetrated, often by repeatedlybranched tracks: the point of divergence of the arms of a branched track, in both mesophyll and vascular bundles, occurs only in large inter- or intracellular spaces where the lobular sheath or track is present. Three types of track are common in the vascular tissue:the lobular, the membraneous, and a saliva-filled type. The majority of stylet bundles terminate in the phloem. Rotation of the stylet bundle about its longitudal axis is generally absent.

A peculiar malignant liver adenoma

In October 1909, the speaker met at an autopsy a completely peculiar liver damage in a 21-year-old child, more than 3 years of diarrhea and a significant increase in the spleen (with ascites); the last one was removed to him, and the patient died from septic peritonitis. The liver was smooth, 1270 gm. vѣsom and extremely soft; in the portal vein and she herself turned out to be clogged with blood clots, red in the liver, and whitish in the drink itself. Liver tissue appeared to be red and och. soft, in some places whitish-yellowish color; areas of the last days formed nodes in the forms of cerebral convolutions, enveloping the rays of the Glisson capsule with large veins; the convolutions seemed striated, as if folded from the tubes, vertical to the axis of the crossbeam of the Glisson capsule. Such whitish areas were scattered everywhere and in the red parenchyma, not sharply delimited * from it and without lobular structure.