Application of Environmental DNA (eDNA) Metabarcoding Method to Identify Threatened Sulawesi Mammal Based on 12S rRNA Gene

Species detection and identification is a crucial steps in biodiversity assessment. Traditional methods are often invasive and resource intensive. The number of studies demonstrating successful of eDNA metabarcoding approach in species identification has increased rapidly in recent years. Some of large terrestrial mammals have reportedly utilize natural salt licks as a source of minerals in the diet and its genetic material left in the environment can be used to identify species from this site. An eDNA metabarcoding protocol had been carried out to identify Sulawesi mammals from Adudu natural salt-licks, Nantu Wildlife Reserve, Gorontalo. Environmental DNA were extracted from water samples, Amplicon libraries were prepared by PCR amplification and Illumina MiSeq high throughput sequencing. Reads processing and taxonomic assignment carried out in two bioinformatics packages, PipeCraft-1.0 and OBITools-2.11. Two endangered Sulawesi mammals species had been identified, i.e. lowland anoa (Bubalus depressicornis) and babirusa (Babyrousa babyrussa). The accuracy of mammal species identification using eDNA metabarcoding is affected by rigorous experimental procedures, DNA marker reliability, and availability of reference sequence database.

Cyclicity and individuality in the salt licking by moose, Alces alces (Linnaeus, 1758), in the North-West of the Russian Federation

Modern remote sensing methods contribute to the study of previously unexplored features of the vital activity of animals, including mineral nutrition of the largest terrestrial mammals in the North-West of the Russian Federation. In the article, seasonal observations of individual moose [Alces alces (Linnaeus, 1758)] on artificial salt licks were analyzed. The attendance of salt licks was monitored using automatic photo-video recorders (cam-era traps) from 2015 to 2020 on the territory of various hunting farms in 3 districts of the Leningrad Province. The annual, seasonal and daily cyclicity in the use of mineral feeding places by moose was revealed. For this research, from the total sample (n = 372) of registered individuals, 118 most reliably identified moose were specially selected, of which 72 (61%) showed cyclicity in salt licking with a certain frequency. In the remaining 46 individuals, the cyclicity was not observed for explainable objective reasons. Females, to a greater extent than males, are more settled in relation to places of mineral feeding, which is indirectly confirmed by the percentage in the selected groups. The time intervals between the periods of salt licking vary individually. Based on similar patterns of attendance inherent in most individuals, the nature of visiting artificial salt licks by moose during the active period of salt licking (April-November) is as follows: each “group of visits” consists of several (1–11 times per day) periods of salt licking for 1–5 days in a row, followed by a break of 10 to 20 days, followed by a repetition of the “group of visits”.

Impact of natural salt lick on the home range of Panthera tigris at the Royal Belum Rainforest, Malaysia

Natural salt lick (sira) is a strategic localisation for ecological wildlife assemblage to exhibit geophagy which may act as a population dynamic buffer of prey and predators. Undoubtedly, many agree that geophagy at natural licks is linked to nutritional ecology, health and assembly places facilitating social interaction of its users. Overall, natural salt licks not only save energy of obtaining nutrient leading to health maintenance but also forms the basis of population persistence. The Royal Belum Rainforest, Malaysia (Royal Belum) is a typical tropical rainforest in Malaysia rich in wildlife which are mainly concentrated around the natural salt lick. Since this is one of the most stable fauna ecology forest in Malaysia, it is timely to assess its impact on the Malayan tiger (Panthera tigris) home range dynamics. The three-potential home ranges of the Malayan tiger in this rainforest were selected based on animal trails or foot prints surrounding the salt lick viz (e.g. Sira Kuak and Sira Batu; Sira Rambai and Sira Buluh and Sira Papan) as well as previous sightings of a Malayan tiger in the area, whose movement is dependent on the density and distribution of prey. Camera traps were placed at potential animal trails surrounding the salt lick to capture any encountered wildlife species within the area of the camera placements. Results showed that all home ranges of Malayan tiger were of no significance for large bodied prey availability such as sambar deer (Rusa unicolor), and smaller prey such as muntjacs (Muntiacus muntjac) and wild boar (Sus scrofa). Interestingly, all home range harbour the Malayan tiger as the only sole predator. The non-significance of prey availability at each home range is attributed to the decline of the Malayan tiger in the rainforest since tigers are dependant on the movement of its preferred prey surrounding natural salt licks. Thus, the information from this study offers fundamental knowledge on the importance of prey-predator interaction at salt lick which will help in designing strategy in rewilding or rehabilitation programs of the Malayan tiger at the Royal Belum Rainforest.

Physical factors at salt licks influenced the frequency of wildlife visitation in the Malaysian tropical rainforest

Mineral reservoirs or salt licks are commonly used by wildlife to regulate the concentration of salt and minerals in their bodies. Salt lick utilisation is known to be influenced by the chemical composition, but information on their physical properties, particularly vegetation surrounding the salt licks is scarce. In this study, physical factors and wildlife utilisation at two natural salt licks (SPU and SPS) located in Perak, Malaysia, were determined. Wildlife visitation data were retrieved from camera traps. SPU exhibits two reservoirs represented by rocky and clay substrate, with minimally dense vegetation dominated by hardwood species and climbers. SPS encompasses muddy topsoil, open canopy cover, with highly dense forest floor vegetation. The wildlife survey shows a higher frequency of visitation in SPS, especially by ungulates, potentially due to dense understorey foliage that provides foraging sites for these animals. SPU exhibits more diverse but less frequent wildlife species, particularly primates, carnivores, and avian groups. High emergence and closed canopy cover at this lick serve as perching sites for primates and birds, while less dense understory vegetation could aid in prey detection among carnivore. Regardless of their physical assemblages, salt licks are an essential local hotspot for wildlife, therefore, elucidating the need to prioritise conservation areas by maximising the complementarities of salt licks.