scholarly journals Komponen Serat Sabut Kelapa yang Difermentasi Menggunakan Mikroba Pencerna Serat dari Rumen Kerbau

2020 ◽  
Vol 20 (1) ◽  
pp. 1-8
Author(s):  
Limbang Kustiawan Nuswantara ◽  
Sunarso Sunarso ◽  
Mukh Arifin ◽  
Agus Setiadi

ABSTRAK. Penelitian ini bertujuan untuk mengkaji pengaruh perbedaan lama pemeraman dan aras starter mikroba pencerna serat dari rumen kerbau terhadap komponen serat dan perubahan struktur jaringan sabut kelapa fermentasi. Percobaan menggunakan rancangan acak lengkap pola faktorial 3x3 dan 4 ulangan. Fermentasi sabut kelapa menggunakan mikroba pencerna serat dari rumen kerbau sebagai starter dengan perlakuan aras starter (0, 2,5 dan 5%) dan lama peram (0, 7 dan 14 hari). Parameter yang diamati adalah komponen serat dan perubahan struktur jaringan. Data dianalisis menggunakan analisis ragam, dilanjutkan uji jarak berganda Duncan. Hasil penelitian menunjukkan tidak terdapat pengaruh interaksi (P0,05) antara perbedaan aras starter dan lama peram terhadap kadar neutral detergent fiber (NDF), acid detergent fiber (ADF), hemiselulosa, selulosa dan lignin. Kesimpulan penelitian ini adalah penurunan kadar komponen serat serta peningkatan kerusakan jaringan seiring dengan peningkatan aras starter dan lama pemeraman. Hal ini disebabkan oleh penurunan kadar selulosa dan lignin sedangkan kadar hemiselulosa tidak dipengaruhi. Hal ini didukung oleh pengamatan terhadap perubahan struktur jaringan.  (Fiber components of fermented coconut husk using fibre degrading microbes from buffalo rumen) ABSTRACT. This study aimed to examine the effect of different fermentation periods and levels of fiber-degrading microbial starter from buffalo rumen fluid on fiber component and tissue structure alteration of coconut husk. The experimental design used was factorial randomized complete 3x3 and 4 replications. The coconut husk fermentation was using fiber-degrading microbial obtained from buffalo rumen fluid as a starter with different treatments of starter levels (0, 2.5 and 5%) and fermentation periods (0, 7 and 14 days). Parameters observed were fiber component and tissue structure alteration. The data were analyzed by analysis of variance and continued by Duncan’s Multiple Range Test. The results of this research showed that there was no interaction effect (P0.05) between starter level and fermentation period on NDF, ADF, hemicellulose, cellulose and lignin contents. The conclusion of this research was decreased fiber component content and damage to tissue structure of coconut husk along with increased starter level and fermentation period. This is caused by decreased levels of cellulose and lignin while hemicellulose levels were not affected. This is supported by observations of changes in tissue structure alteration.

Author(s):  
Ivone Rodrigues da Silva ◽  
Francirose Shigaki ◽  
Rosane Cláudia Rodrigues ◽  
Ana Paula Ribeiro Jesus ◽  
Clésio dos Santos Costa ◽  
...  

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.


2012 ◽  
Vol 35 (3) ◽  
pp. 165
Author(s):  
Dewi Puspita Candrasari ◽  
Subur Priyono Sasmito Budhi ◽  
Hari Hartadi

<p>Penelitian bertujuan untuk konservasi hasil sisa tanaman pertanian berupa bagas tebu, meningkatkan kecernaan dan meningkatkan nilai N bagas tebu sehingga dapat digunakan sebagai pakan. Level Ca(OH)2 (b/b) yang digunakan<br />sebesar 0, 3, dan 6%. Level urea yang digunakan adalah 0, 3, dan 6%. Masing–masing perlakuan diulang sebanyak 3 kali. Penelitian menggunakan rancangan acak lengkap pola faktorial 3 x 3. Setelah diberi perlakuan, bagas tebu<br />ditempatkan pada kantong plastik polyethylene dan disimpan selama 21 hari. Data yang diperoleh dianalisis variansi dan apabila terdapat perbedaan antara perlakuan akan diuji lanjut dengan Duncan’s New Multiple Range Test. Dari<br />penelitian diperoleh hasil kandungan bahan kering (BK), bahan organik (BO), protein kasar (PK), serat kasar (SK), lemak kasar (LK), bahan ekstrak tanpa nitrogen (BETN), neutral detergent fiber (NDF), dan acid detergent fiber (ADF)<br />masing-masing berkisar 45,06-48,51%, 90,05-95,67%, 1,40-4,06%, 50,37-53,50%, 1,33-3,33%, 32,73-39,94%, 80,00-90,69%, dan 61,50-65,71%. Kecernaan bahan kering (KcBK) dan kecernaan bahan organik (KcBO) bagas tebu<br />diperoleh sebesar 24,24-39,60% dan 24,88-40,24%. Disimpulkan bahwa bagas tebu yang ditambah urea 3% dan kalsium hidroksida 6% paling baik dilihat dari kandungan PK.</p><p><br />(Kata kunci: Bagas tebu, Kalsium hidroksida, Urea, NDF, ADF, Kecernaan in vitro)<br /><br /></p>


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 451-451
Author(s):  
YangYi Hao ◽  
Shuai Huang ◽  
Shengli Li

Abstract The primiparity and multiparity dairy cattle were fed in the same diet and different barn in the scaled farm because heifer has a lower social hierarchy. There is little attention to the rumen function difference of these cattle. Here we selected thirty-two dairy cattle and separated them into four groups: prime-prepartum (PP), prime-lactation (PL), multi-prepartum (MP), and multi-lactation (ML) (n=8). The PP and MP groups were given high-roughage diets, and the other two groups were given high-grain diets. The rumen fluid and feces were sampled from each animal. The rumen fluid enzyme activity, fermentation profile, and apparent digestibility were analyzed using SAS’s PROC MIXED model (SAS version 9.4, SAS Institute Inc., Cary, NC, USA). β-glucosidase and amylase were affected by the parity and diet (P &lt; 0.05). Cellulase was affected by the parity, and dehydrogenase was affected by parity and diet interaction (P &lt; 0.05). Rumen pH, NH3-N, microbial crude protein (MCP), isovalerate, and acetate/propionate (A/P) were affected by parity (P &lt; 0.05). MCP and volatile fatty acid were affected by the diets (P &lt; 0.05). The rumen pH, acetate, propionate, and butyrate were affected by parity and diet interaction (P &lt; 0.05). The apparent digestibility of the neutral detergent fiber, acid detergent fiber, crude protein (CP), and ether extract (EE) were affected by the diet and the interaction of diet and parity (P &lt; 0.05). The CP and EE apparent digestibility were only affected by the parity (P &lt; 0.05). Our results indicated beside the diet, the parity also affected the rumen function and apparent digestibility. Although the dairy cattle in the same physical stage, the parity effects on the rumen function and digestibility should be considered when making the diet formula.


2016 ◽  
Vol 13 (1) ◽  
pp. 19
Author(s):  
Andika Mulya ◽  
Dewi Febrina ◽  
Triani Adelina

Harvesting bananas will produce stems and stump that can be used as an alternative feed. One processing techniques tomaintain the nutritional value is silage. This study aims to determine the content of the fiber fraction ie. Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), lignin, cellulose and hemicellulose from bananas waste silage with the composition of the substrate and the different levels of molasses.  Research using a Completely Randomized Design factorial pattern with two replications. Factor A : the composition of the substrate, A1. stumps 100% :  stems 0%; A2. 50% stems : 50% stumps; A3. 0% stumps : 100% stems.  Factor B : the addition of molasses  0%; 2.5% and 5%. Data were analyzed using a completely randomized factorial design, the difference between treatments tested Duncan's Multiple Range Test (DMRT). The results showed banana waste silage with the composition of the substrate and molasses with different levels have moderate physical qualities and fiber fractions are NDF 57.86-89.63%; ADF 34.44-46.20%; lignin 11.75-17.19%; cellulose 15.10-32.34%; hemicellulose 11.66-55.18%. Silage that contains 100% of banana stem with the addition of 5% molasses is the best result. 


Author(s):  
P. M, Lunagariya ◽  
R. S. Gupta ◽  
S. V. Shah ◽  
Y. G. Patel

The study was planned to evaluate the effect of exogenous fibrolytic enzymes (EFE) supplementation for 56 days @ 240 mg/kg total mixed ration (TMR) on digestibility of dry matter and nutrients in dairy cows. Six dry non-pregnant cows were assigned in each treatment with and without EFE. The digestibility trial of seven days was conducted after 49 days of feeding. Dry matter and nutrients intake of cows was not influenced by EFE. The supplementation of EFE had improved digestibility of dry matter, organic matter, crude fiber, neutral detergent fiber, cellulose (p less than 0.01), as well as digestibility of nitrogen-free extract and acid detergent fiber, was also higher (pless than 0.05). The body weight gain of cows was higher on the supplementation of EFE in TMR. The study concluded that feeding exogenous fibrolytic enzymes (240 mg/kg) supplemented TMR improved digestibility of dry matter and nutrients, which was reflected as higher body weight gain in dry non-pregnant Gir and crossbred dairy cows.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 666
Author(s):  
Rafael Font ◽  
Mercedes del Río-Celestino ◽  
Diego Luna ◽  
Juan Gil ◽  
Antonio de Haro-Bailón

The near-infrared spectroscopy (NIRS) combined with modified partial least squares (modified PLS) regression was used for determining the neutral detergent fiber (NDF) and the acid detergent fiber (ADF) fractions of the chickpea (Cicer arietinum L.) seed. Fifty chickpea accessions (24 desi and 26 kabuli types) and fifty recombinant inbred lines F5:6 derived from a kabuli × desi cross were evaluated for NDF and ADF, and scanned by NIRS. NDF and ADF values were regressed against different spectral transformations by modified partial least squares regression. The coefficients of determination in the cross-validation and the standard deviation from the standard error of cross-validation ratio were, for NDF, 0.91 and 3.37, and for ADF, 0.98 and 6.73, respectively, showing the high potential of NIRS to assess these components in chickpea for screening (NDF) or quality control (ADF) purposes. The spectral information provided by different chromophores existing in the chickpea seed highly correlated with the NDF and ADF composition of the seed, and, thus, those electronic transitions are highly influenced on model fitting for fiber.


2017 ◽  
Vol 52 (6) ◽  
pp. 393-400
Author(s):  
Rafael Lopes da Silva ◽  
Eric Portilho de Araújo ◽  
Mariucha Karina Honório Ribeiro Rocha ◽  
Flavia Mota Damasceno ◽  
Jakeline Marcela Azambuja de Freitas ◽  
...  

Abstract: The objective of this work was to evaluate the diet components - crude fiber, neutral detergent fiber, acid detergent fiber, hemicellulose, cellulose, and lignin - as internal indicators in the determination of the apparent digestibility coefficients of dry matter, gross energy, and of the nutrients - crude protein, phosphorus, and amino acids - in Nile tilapia (Oreochromis niloticus). Groups of ten juveniles (80.3±1 g) were randomly distributed in six tanks of 250 L and fed two practical diets, either of a plant-origin diet or of a plant- and animal-origin diet. Both diets were supplemented with 0.1% chromium (III) oxide (Cr2O3). Faeces were collected by the modified Guelph system. The apparent digestibility coefficients were determined by the content difference of the internal indicators, present in the diets and faeces, and compared by Dunnett’s test to those obtained by the use of Cr2O3. Cellulose was effective in the determination of the apparent digestibility of dry matter, energy, and nutrients of both experimental diets; and the acid detergent fiber was effective only for the diet composed exclusively of plant-origin ingredients. The use of crude fiber, neutral detergent fiber, hemicellulose, and lignin as digestibility indicators was inefficient for the analyzed nutrients of both diets. Therefore, cellulose is the most suitable indicator for digestibility evaluation in Nile tilapia.


2016 ◽  
Vol 37 (4Supl1) ◽  
pp. 2589
Author(s):  
Marjorrie Augusto de Souza ◽  
Edenio Detmann ◽  
Gabriel Cipriano Rocha ◽  
Marcia De Oliveira Franco ◽  
Erick Darlisson Batista ◽  
...  

The objective of this collaborative study was to evaluate the indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) content in feeds in six laboratories from institutions linked to the National Institute of Science and Technology in Animal Science (INCT-CA). Six feeds were evaluated: signal grass hay, sugarcane, corn silage, soybean meal, corn and citrus pulp. Estimated levels of iNDF and iADF proved to be dependent on the laboratory in which the analysis was performed. It was found that differences between laboratories ranged from 2.40 percentage points for soybean meal to 8.05 percentage points for sugarcane for iNDF analysis and from 1.79 percentage points for corn to 10.06 percentage points for hay for iADF analysis. It was observed that the individual evaluation of each material, the total random variation of the results between laboratories ranged from 88.75 to 96.77% and 88.75 to 98.40% for iNDF and iADF analysis, respectively. The iNDF and iADF levels are dependent on the interaction effect between the evaluated material and laboratory and have low reproducibility; this may be a consequence of the differences between the methods practiced by each laboratory, demonstrating lack of standardization of procedures used by the laboratories.


2014 ◽  
Vol 38 (5) ◽  
pp. 497-505 ◽  
Author(s):  
Adriana de Souza Martins ◽  
Juliane Ribeiro de Oliveira ◽  
Marili Lopes Lederer ◽  
José Luís Moletta ◽  
Shivelly Los Galetto ◽  
...  

Due to the seasonal cycle of forage, the use of silage to feed animals provides nutrients throughout the year. However, its quality can be improved with the inclusion of additives and other products. Glycerol is a rich source of energy and present a high efficiency of utilization by animals. The purpose of this work was to evaluate the effect of glycerol inclusion on the chemical and fermentation characteristics of corn and sunflower silages. Two silage sources (maize and sunflower) were used and four levels of glycerol inclusion (0, 15, 30 and 45%) based on dry matter were carried out. The experimental design was completely randomized in a 2 x 4 factorial arrangement with five replications. The pH values and chemical composition of corn and sunflower silages were determined. In both silages there was increment of dry matter, non-fiber carbohydrates and total digestible nutrients (TDN) added to a reduction of crude protein, neutral detergent fiber and acid detergent fiber due to the glycerol inclusion. The corn silage required 45% glycerol to achieve the TDN level of the sunflower silage. The glycerol addition contributed to the increase in the nutritional value, offsetting loss of quality in the ensiling process.


1992 ◽  
Vol 72 (4) ◽  
pp. 881-889 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. Bittman ◽  
L. J. Fisher

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P < 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P < 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers


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