MetaRNAseq analysis of surti buffalo rumen content reveals that transcriptionally active microorganisms need not be abundant

This research aimed to determine the fungi colonies total on the rumen content of cattle and buffaloes with the addition of leaves and oil palm fronds incubated for 7 days. Each of the contents of the rumen was added with molasses, soybean soaking water as an energy source for microorganisms, as well as the addition of oil palm fronds and palm leaves to expect the type of fungus that is expected to grow. Calculation of the total colonies fungi in the rumen contents was carried out using total plate count with the dilution method. Complete Random Design by Factorial used to analyze the statistic data. Factor A was the type of rumen content (A1 = rumen of cattle, A2 = buffalo rumen), factor B was the addition of palm fronds (B1 = only rumen contents, B2 = palm oil fronds, B3 = palm leaves, and B4 = palm oil fronds and palm leaves). The results showed that the total number of fungal colonies was significantly higher in the rumen contents of cattle with an average of 509.17 104 CFU / ml. The highest total number of fungal colonies was found in rumen contents with the addition of palm oil fronds, with an average colony number of 655.83 x104 CFU / ml, while the lowest was found in buffalo rumen contents with the addition of palm fronds and palm leaves (106.67x104 CFU / ml). The addition of oil palm fronds and palm leaves to the rumen contents did not have a different effect (p> 0.05).

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Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen

Advances in Bioscience and Biotechnology ◽

10.4236/abb.2014.53030 ◽

2014 ◽

Vol 05 (03) ◽

pp. 235-245 ◽

Cited By ~ 5

Author(s):

Neelam M. Nathani ◽

Srinivas M. Duggirala ◽

Vaibhav D. Bhatt ◽

Jay KaPatel ◽

Chaitanya G. Joshi

Keyword(s):

Genomic Analysis ◽

Surti Buffalo ◽

Buffalo Rumen ◽

Bacillus Nealsonii

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Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

3 Biotech ◽

10.1007/s13205-017-0895-2 ◽

2017 ◽

Vol 7 (4) ◽

Cited By ~ 5

Author(s):

Ravi K. Shah ◽

Amrutlal K. Patel ◽

Deepti M. Davla ◽

Ishan K. Parikh ◽

Ramalingam B. Subramanian ◽

...

Keyword(s):

Heterologous Expression ◽

Molecular Cloning ◽

Functional Characterization ◽

Cellulolytic Enzyme ◽

Rumen Metagenome ◽

Buffalo Rumen

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The use of cumulative gas production technique to characterize changes in the fermentation characteristics of rumen contents following variable periods of starvation and grazing in dairy cows

Animal Science ◽

10.1017/s135772980005150x ◽

1999 ◽

Vol 69 (3) ◽

pp. 647-655 ◽

Cited By ~ 3

Author(s):

P. Chilibrostet ◽

B. A. Williams ◽

S. Tamminga ◽

S. Calabro

Keyword(s):

Dairy Cows ◽

Dry Matter ◽

Bulk Material ◽

Gas Production ◽

Rumen Content ◽

Starvation Period ◽

Half Time ◽

Production Technique ◽

Grazing Experiment ◽

Time Required

AbstractThe effect of the duration of grazing (experiment 1) and starvation time and placement in the rumen of inert bulk material before grazing (experiment 2), on the rumen content ferment ability, was investigated by means of measuring cumulative gas production. In experiment 1, a comparison was made of four durations of grazing (1, 1·75, 2·50 and 3·25 h) after overnight starvation. Rumen samples taken from the cows after 1 h of grazing had higher values of total accumulated gas with less (P < 0·05) time required to reach the maximum fermentation rate than cows grazed for 3·25 h. Following grazing, a 7·75·h starvation period was imposed on the four treatments. The extent of fermentation was significantly lower (P < 0·01) after starvation than immediately after grazing (49·7 v. 60·8% of incubated dry matter (DM), respectively). Experiment 2 consisted of a factorial combination of two durations of starvation before grazing (16·5 (LS) and 2·5 (SS) h) with the presence or absence in the rumen of 12·5 kg of a synthetic indigestible material. Before grazing the total accumulated gas production was less (P < 0·05) for the LS than for the SS cows. After the grazing session, the total gas of rumen samples from the LS cows was significantly higher (P < 0·05) than for the SS cows.This was in agreement with the observed higher DM intake during grazing and DM rumen pools after grazing in LS cows. For both starvation periods, the presence of inert rumen bulk led to a higher total gas, a shorter half-time and less DM left unfermented. The measurement of fermentation kinetics by cumulative gas production was suitable to detect changes in rumen content fermentation patterns due to the clearance of material from the rumen (effect of starvation) or DM intake during the grazing sessions.

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Performance and Carcass Production of Balinese Ducks Given Rumen Watse Based Biosupplement Rations

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2017.v01.i02.p01 ◽

2018 ◽

Vol 1 (2) ◽

pp. 29

Author(s):

Gusti Ayu Mayani Kristina Dewi ◽

I Nyoman Sutarpa Sutama ◽

I Wayan Wijana ◽

I Made Mudita

Keyword(s):

Rumen Content ◽

Meat Production ◽

Bacterial Isolates ◽

Cellulolytic Bacteria ◽

Randomized Sampling ◽

Bacterial Inoculants ◽

The Village

A study aimed at studying the performance and production of Balinese duck carcasses that were given rations containing biosupplement-based waste of rumen content, conducted in the village of Peguyangan, Denpasar, Bali, for 3 months. Four biosupplements produced in this study were 1) BR0 = biosupplement with fermented rumen based waste without bacterial isolates, 2) BR1 = biosupplement with fermented rumen based waste with superior 1 cellulolytic bacteria inoculant (BR3.5), 3) BR2 = biosupplement with fermented rumen based waste with superior 2 cellulolytic bacterial inoculants (BR3.3), 4) BRmix = biosupplement with fermented rumen based waste with combination of superior cellulolytic bacteria 1 and 2 (BR3.5 and BR3.3), The study was carried out with Completely Randomized Sampling/CR Design with 5 treatments and 3 repetitions, in which each repetition consisted of 5 Balinese ducklings aged 2 weeks. The treatment given was to ducks given supplemented ration with BR0; BR1; BR2; BRmix, respectively for the treatment of R1; R2; R3; and R4, as well as ducks that were given basal rations without supplementation (R5). The results showed that the performance of duck that received treatment of R2 was significantly better (P<0,05) than R1, R3, R4 and R5, while the carcass butching weight and fat weight were not significantly different (P>0,05) . Based on the results obtained, it can be concluded; 1) the performance of Balinese ducks receiving ration biosupplement of R2 is better compared to R1, R3, R4 and R5; 2) Meat production/ducks carcasses to the weight of butchery, carcass production, non-carcass parts and body fats are not significantly real between all treatments.

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Effect of diet on maintenance of acid-basal balance in blood of dairy cows

Veterinarski glasnik ◽

10.2298/vetgl0304159g ◽

2003 ◽

Vol 57 (3-4) ◽

pp. 159-172

Author(s):

T. Gaál

Keyword(s):

Metabolic Acidosis ◽

Dairy Cows ◽

High Performance ◽

Rumen Content ◽

Rumen Acidosis

High-performance breeds of ruminants often exhibit production disorders which can be accompanied by a disturbed acid-basal balance. Most of the disorders in the acid-basal balance are closely related to digressions in the diet norms of these animals. A deficiency or surplus of energy equally cause disorders in the acid-basal status of the organism. Metabolic acidosis is the most frequent of the four types of basic disorders in the acid-basal balance in ruminants. It appears as a consequence of rumen acidosis, ketosis, or diarrhea. Acute disorders in the acid-basal balance are far more dangerous than chronic ones. Therapy of the basic diseases is generally sufficient compensation for the effects of the acid-basal disorders, but in certain cases it is necessary to perform alkalization, that is, acidification of the rumen content using the necessary preparations.

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Data of bacterial community dynamics resulting from total rumen content exchange in beef cattle

BMC Research Notes ◽

10.1186/s13104-021-05726-1 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Brooke A. Clemmons ◽

Madison T. Henniger ◽

Phillip R. Myer

Keyword(s):

Bacterial Community ◽

Preliminary Data ◽

Community Dynamics ◽

Hypervariable Region ◽

Rumen Content ◽

Rrna Gene ◽

Lactating Cows ◽

Rumen Microbiome ◽

Bacterial Community Dynamics

Abstract Objectives Extensive efforts have been made to characterize the rumen microbiome under various conditions. However, few studies have addressed the long-term impacts of ruminal microbiome dysbiosis and the extent of host control over microbiome stability. These data can also inform host-microbial symbioses. The objective was to develop preliminary data to measure the changes that occur in the rumen bacterial communities following a rumen content exchange to understand the effects major perturbations may impart upon the rumen microbiome, which may be host-driven. Data description We report here an initial rumen content exchange between two SimAngus (Simmental/Angus) non-pregnant, non-lactating cows of ~ 6 years of age weighing 603.4 ± 37.5 kg. To measure bacterial community succession and acclimation following the exchange, rumen content was collected via rumen cannula at the beginning of the study immediately prior to and following the rumen content exchange, and weekly for 12 weeks. The V4 hypervariable region of the 16S rRNA gene was targeted for DNA sequencing and bacterial analysis. Over 12 weeks, numerous genera and diversity varied, before partial return to pre-exchange metrics. These preliminary data help support potential host control for the rumen microbiome, aiding in efforts to define bovine host-microbe relationships.

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