Snack bars made from black soybean and black rice could be used as a food product to
complement the energy and nutrients needs. Both have anthocyanins content in black
soybean and black rice as well as the potential as an antimicrobial agent. The purpose of
this study was to obtain an optimal formula, evaluate the sensory acceptance, analyze the
total monomeric anthocyanin content of the snack bars, and compare the growth of
probiotic and pathogenic bacteria in anthocyanins crude extracts from the snack bars. The
production of snack bars was done by using three variations of main ingredients, including
30% of black soybean + 70% of black rice (formula 1), 50% of black soybean + 50% of
black rice (formula 2), and 70% of black soybean + 30% of black rice (formula 3). The
results performed that consumers acceptance for formulas 2 and 3 was not significantly
different (P > 0.05), but significantly higher (P < 0.05) than formula 1. Total monomeric
anthocyanin content analyzed by the pH differential method in these three snack bars
formulas had no significant difference (P > 0.05). The growth analysis of probiotic and
pathogenic bacteria showed that the percentage of growth inhibition of Escherichia coli
and Salmonella enterica serovar Typhi bacteria was significantly higher (P = 0.00)
compared to Lactobacillus acidophilus. Also, formulas 1 and 2 could significantly inhibit
E. coli and S. enterica ser. Typhi bacteria (P = 0.00) compared to formula 3. It could be
concluded that formula 2 showed the best snack bar based on sensory evaluation and
pathogenic bacteria inhibition assay.