Recent spruce (Picea abies) treeline ecotonal progression since the late1980s in the Swedish Scandes-a repeat photographic narration and analysis

This study reports a case of climate-mediated transformation and physiognomic progression of the Norway spruce (Picea abies) treeline ecotone since the mid-1990s in the Swedish Scandes. The methods include repeat photography and foliation estimates of old-established clonal spruces. An air and soil temperature nadir by the 1980s had caused extensive needle and shoot mortality, evident at the landscape-scale. Subsequent winter and summer temperature rises induced a striking canopy recovery, including densification and vertical growth. Release from low soil temperature stress appears as instrumental for canopy progression and shift from stunted growth to erect tree forms. Seed-based regeneration of new individuals has been virtually nil and the ecotone appears to be spatially stable. Ongoing ecotonal shifts has the character of growth form transformations in accordance with climatic conjunctures.

Pilot Study to Evaluate Performance of Frost-Yuzu Fruit Trees under Protected Cultivation

This study was initiated to observe the performance of yuzu (Citrusjunos Sieb. ex Tanaka) fruit trees when affected by a late freezing in 2018 and to evaluate the recovery of frost-damaged trees during post management under protected cultivation. A—4.9 °C of minimum daily temperature and 40-day drought occurred during dormancy, which then received heavy precipitation between early- and mid-March, with 15 m s−1 more than maximum instantaneous wind speeds frequently observed. This resulted in observed decreases in height, width and volume as well as in fruiting, fruit weight and yield, as well as yield index in 60–90% defoliated yuzu trees, in addition to higher rates of shoot dieback compared to trees that experienced only 0–30% defoliation. Lower performance and recovery rates of trees grown on flat land compared to trees on sloped land were also observed. Tree and net windbreaks did not significantly affect tree vegetative growth and fruit productivity but were found to have lowered shoot mortality in 2018 and 2019. Mulch with an irrigation after freezing or foliar urea application was shown to effectively increase vegetative tree growth and fruit productivity and reduce shoot mortality.

Rising CO2 in the field does not offset warming or drought constraints on leaf growth of a C3 forage

The regrowth of a C3 forage Stylosanthes capitata in a rain-fed field provided the weekly data collection in the southeast of Brazil during a heat event in autumn. A system named Trop-T-FACE simulated the free-air carbon dioxide enrichment and the enhanced temperature in four climatic regimes: current atmospheric conditions (Control), CO2 enrichment (600 ppm, eC), warming (2 °C above ambient canopy temperature, eT), and a combination of eC + eT. The area and biomass of foliage per shoot decreased, and the number of flowers per shoot and flowered ramification increased under single eC treatment besides the increment in palisade parenchyma of leaves. Increased investment in flowering in eC occurred notably when the soil water content was higher than 0.30 m3 m–3. Single eT treatment also impaired the area or biomass of foliage production per shoot, raised the shoot mortality, and promoted the increment of the spongy leaf parenchyma. There was some mitigation of the adverse effects of foliage production of eT or eC in eC + eT, but under this combined treatment, the shoot mortality also increased. Changes in leaf tissues under eC or eT or some mitigation of adverse effects in eC + eT did not offset the constraints on leaf growth per shoot. The harmful impact on foliage production by eC, eT, or eC + eT under rain-fed conditions indicated no advantages for feeding the livestock with the C3 forage S. capitata in expected climate change under field conditions.

Assessment of Freeze Injury of Grapevine Green Tissues in Response to Cultivars and a Cryoprotectant Product

Spring frosts and subsequent crop losses threaten the economic sustainability of fruit crop producers all over the world. This study used a controlled-freezing technique to impose a post-budburst freezing stress to grapevine shoots forced from one-node cuttings [‘Albariño’, ‘Cabernet Franc’, ‘Cabernet Sauvignon’, and ‘Pinot Grigio’ (Vitis vinifera)] and whole plants [‘Noiret’ (Vitis hybrid)]. Our goal was to investigate the incidence of freeze injury among cultivars, stage of phenological development, and a potassium salt-based fertilizer (KDL) with potential cryoprotectant activity. Among the V. vinifera cultivars, the incidence of mortality of shoots exposed to −3.5 °C was highest for ‘Albariño’ (71%) and lowest for ‘Cabernet Sauvignon’ (51%). Cuttings sprayed with KDL 24 hours before cold temperature exposure exhibited 16% lower shoot mortality and lower osmotic potential (Ψs) (−0.92 MPa) than the unsprayed cuttings (−0.77 MPa). However, application of KDL did not impact shoot mortality for whole ‘Noiret’ vines. Mortality for ‘Noiret’ shoots greatly increased with the advancement of phenological development, ranging from 10% in wooly buds to 78% in shoots ≈10-cm long. The practical significance of KDL remains questionable; cultivar selection still appears to be a more reliable method for avoiding spring frost, by planting late bursting cultivars in more frost-prone areas.

CO₂ and nutrient-driven changes across multiple levels of organization in <i>Zostera noltii</i> ecosystems

Increasing evidence emphasizes that the effects of human impacts on ecosystems must be investigated using designs that incorporate the responses across levels of biological organization as well as the effects of multiple stressors. Here we implemented a mesocosm experiment to investigate how the individual and interactive effects of CO2 enrichment and eutrophication scale-up from changes in primary producers at the individual (biochemistry) or population level (production, reproduction, and/or abundance) to higher levels of community (macroalgae abundance, herbivory, and global metabolism), and ecosystem organization (detritus release and carbon sink capacity). The responses of Zostera noltii seagrass meadows growing in low- and high-nutrient field conditions were compared. In both meadows, the expected CO2 benefits on Z. noltii leaf production were suppressed by epiphyte overgrowth, with no direct CO2 effect on plant biochemistry or population-level traits. Multi-level meadow response to nutrients was faster and stronger than to CO2. Nutrient enrichment promoted the nutritional quality of Z. noltii (high N, low C : N and phenolics), the growth of epiphytic pennate diatoms and purple bacteria, and shoot mortality. In the low-nutrient meadow, individual effects of CO2 and nutrients separately resulted in reduced carbon storage in the sediment, probably due to enhanced microbial degradation of more labile organic matter. These changes, however, had no effect on herbivory or on community metabolism. Interestingly, individual effects of CO2 or nutrient addition on epiphytes, shoot mortality, and carbon storage were attenuated when nutrients and CO2 acted simultaneously. This suggests CO2-induced benefits on eutrophic meadows. In the high-nutrient meadow, a striking shoot decline caused by amphipod overgrazing masked the response to CO2 and nutrient additions. Our results reveal that under future scenarios of CO2, the responses of seagrass ecosystems will be complex and context-dependent, being mediated by epiphyte overgrowth rather than by direct effects on plant biochemistry. Overall, we found that the responses of seagrass meadows to individual and interactive effects of CO2 and nutrient enrichment varied depending on interactions among species and connections between organization levels.