Investigating the Correlation of Microplastic Pollution Between Seawater and Marine Salt Using Micro-Raman Spectroscopy

Microplastics (MPs) are synthetic polymer pieces with a size of less than 5 mm that are ubiquitous in the marine environment. They have been recently detected in several wild species and sea products. An indispensable condiment in human food is marine salt that is produced from seawater. Since toxicity studies report potential health impacts when plastic debris is ingested, more attention must be paid to the MP contamination of sea salt and the underlying reasons for this. The central aim of this study is to evaluate the MPs contamination level of sea salt in correlation with the MPs contamination level of seawater in Vietnamese areas. Micro-Raman spectroscopy was employed to determine MPs in the samples collected from three artificial salt pans of Vietnam. The result revealed the presence of MPs in all study areas—Vung Tau (VT;14.54 MPs/L seawater and 114.67 MPs/kg salt), Ly Nhon (LN; 13.14 MPs/L seawater and 63.59 MPs/kg salt), and Can Thanh (9.42 MPs/L seawater and 93.69 MPs/kg salt). The comparisons highlight close correlations in the percentage, shape, size, and color of MPs, especially polyethylene, polyethylene terephthalate, and polypropylene particles extracted from the seawater and its salt. This study proved that seawater is a plastic pollution source for salts produced by traditional seawater evaporation. The study also alerts the prevalence of MPs in the environment and human consumables, thereby indicating that actions must be taken to reduce the pollution of water sources in Vung Tau and at the UNESCO Can Gio Mangrove Biosphere Reserve and improve salt production and refinery processes in order to minimize the number of MPs in final salt products for safe consumption.

Beyond Archaea: The Table Salt Bacteriome

Commercial table salt is a condiment with food preservative properties by decreasing water activity and increasing osmotic pressure. Salt is also a source of halophilic bacteria and archaea. In the present research, the diversity of halotolerant and halophilic microorganisms was studied in six commercial table salts by culture-dependent and culture-independent techniques. Three table salts were obtained from marine origins: Atlantic Ocean, Mediterranean (Ibiza Island), and Odiel marshes (supermarket marine salt). Other salts supplemented with mineral and nutritional ingredients were also used: Himalayan pink, Hawaiian black, and one with dried vegetables known as Viking salt. The results of 16S rRNA gene sequencing reveal that the salts from marine origins display a similar archaeal taxonomy, but with significant variations among genera. Archaeal taxa Halorubrum, Halobacterium, Hallobellus, Natronomonas, Haloplanus, Halonotius, Halomarina, and Haloarcula were prevalent in those three marine salts. Furthermore, the most abundant archaeal genera present in all salts were Natronomonas, Halolamina, Halonotius, Halapricum, Halobacterium, Haloarcula, and uncultured Halobacterales. Sulfitobacter sp. was the most frequent bacteria, represented almost in all salts. Other genera such as Bacillus, Enterococcus, and Flavobacterium were the most frequent taxa in the Viking, Himalayan pink, and black salts, respectively. Interestingly, the genus Salinibacter was detected only in marine-originated salts. A collection of 76 halotolerant and halophilic bacterial and haloarchaeal species was set by culturing on different media with a broad range of salinity and nutrient composition. Comparing the results of 16S rRNA gene metataxonomic and culturomics revealed that culturable bacteria Acinetobacter, Aquibacillus, Bacillus, Brevundimonas, Fictibacillus, Gracilibacillus, Halobacillus, Micrococcus, Oceanobacillus, Salibacterium, Salinibacter, Terribacillus, Thalassobacillus, and also Archaea Haloarcula, Halobacterium, and Halorubrum were identified at least in one sample by both methods. Our results show that salts from marine origins are dominated by Archaea, whereas salts from other sources or salt supplemented with ingredients are dominated by bacteria.

Combined Pretreatment by Ultrasound and Struvite Precipitation of Raw Substrates: A Strategy to Overcome C/N Ratio Unbalance in Nitrogen-Rich Anaerobic Co-Digestion Systems

The present study aimed to optimize the struvite chemical precipitation process in nitrogen-rich anaerobic co-digestion systems. Struvite precipitation experiments were carried out using a mixture of cattle slurry liquid fraction and sewage sludge, with and without ultrasound pretreatment. Marine salt or MgCl2 were used as magnesium source in NH4+:Mg2+ stoichiometric proportions of 1:1.5 and 1:3. Under the tested conditions, ammonium nitrogen and orthophosphate were removed from the mixed liquor with a maximum observed efficiency of 43% and 92%, respectively, when the ultrasound treatment was applied prior to struvite precipitation, using MgCl2 as source of magnesium (NH4+:Mg2+ of 1:3). The operating time was 40 min. Different pretreatments were tested prior to the biomethanization experiments, struvite precipitation, ultrasound and a combination of both pretreatments. The application of ultrasound (with an energy input of 218 kJ L−1) and struvite precipitation (NH4+:Mg2+ of 1:3) increased the methane content in the biogas by 82% and reduced hydraulic retention time by 28%, when compared to the anaerobic co-digestion assays without pretreatment. The hydrolytic pretreatment increased the bioavailability of nitrogen by 5%, thus enhancing the removal efficiency of ammonium nitrogen by 20%. Consequently, an increase in the carbon to nitrogen ratio was observed, favoring the methanogenesis process.

Salt, sediments and weathering environments in Bunger Hills

Terrestrial environments at Bunger Hills, East Antarctica, vary from vegetation-rich, little-weathered rock surfaces retaining glacial polish and striations near the glacier and ice-sheet margins to salty, vegetation-poor, extensively weathered regions near to and downwind of marine bays and inlets. Weathering forms include tafoni and orientated pits, which record former wind directions. Although salts are found all over Bunger Hills, the strongly weathered area is coincident with the distribution of halite (NaCl) and thenardite (Na2SO4), both of which are derived from seawater and marine salt spray. Salts elsewhere in Bunger Hills are either subglacial calcium carbonates or rock weathering products including gypsum (CaSO4⋅2H2O) and a range of rarer minerals. These other salt minerals do not weather rocks and sediment. The distribution of halite and thenardite acts as a major control on the geomorphology, sediment geochemistry and biogeography of Bunger Hills.

Tafoni show postglacial and modern wind azimuths that are similar at Bunger Hills

The directions of strong winds are important for the distribution of marine salt spray, rock weathering, lake chemistry and the distribution of vegetation in Bunger Hills, a coastal ice-free oasis in East Antarctica. Present-day strong winds (> 10 m s−1) dominantly blow from 118 ± 21 degrees true (°T; ± 1 SD). Orientated tafoni (weathering pits) might form in bedrock surfaces by salt and ice crystallization, thermal stress and saltating sand particles, recording the orientation of a strongly directional wind field since the last deglaciation, which commenced > 30 000 years ago. The orientations of these tafoni, at 101 ± 18°T for 686 measurements at 28 sites, are indistinguishable from the direction of modern-day strong winds (> 10 m s−1), indicating that the orientation of the slope of the ice sheet has been stable throughout the last 10 000 years during the Holocene.

Microparticles in Table Salt: Levels and Chemical Composition of the Smallest Dimensional Fraction

This study evaluates the chemical composition of microplastic materials (MPs) and non-synthetic particles in different table salts of marine origin by the µFT-IR technique. This research focuses on the microparticles fraction within 10–150 µm of size. Eleven commercial trademarks coming from Italy (IT = 6) and Croatia (CRO = 5) were grouped in two different cost ranges, cheap (n = 5) and expensive (n = 6) and were analysed in replicates (n = 3). Levels and chemical composition of microparticles measured in commercial products were correlated on a statistical basis to some factors of variability of potential scientific interest (geographical origin of marine salt, cost of commercial products, etc.). Results of analyses performed on the tested size fraction of microparticles (10–150 µm) evidence that: (i) levels of MPs are within 0.17–0.32 items/g (IT) and 0.07–0.20 items/g (CRO); (ii) non-synthetic particles detected are mostly made by fibres made of cellulose acetate; (iii) Nations show a different chemical composition of MPs recovered in analysed trademarks (PET and PVC from Italy; PA, PP, and nylon from Croatia); (iv) the annual amount ingested by humans from marine salt consumption ranges between 131.4–372.3 items/y (CRO) and 306.6–580.35 items/y (IT) considering a dose of 5 g of salt per day; (v) statistics performed on factors of interest evidenced that the geographical origin of marine salt do not affect neither levels nor chemical composition of MPs in tested trademarks; while slight correlations are recorded with non-synthetic particles. Further studies are needed to better explore on statistical basis if both levels and chemical composition of MPs in table salts of marine origin can be used or not as good indicators of marine pollution.