A study to assess the level of knowledge regarding umbilical cord stem cell banking among staff nurses at selected Hospitals of Haridwar Uttrakhand

A descriptive study was conducted to assess the level of knowledge regarding umbilical cord stem cell banking among staff nurses at selected Hospitals of Haridwar Uttrakhand A non-experimental research design was used assess the knowledge regarding umbilical cord stem cell banking among staff nurses. The sample consists of 104 staff nurses of selected hospitals who were available at the time of study. Purposive sampling technique was used for selection of samples. The data was collected using self-structured questionnaire. Results depicted that majority of staff nurses had inadequate knowledge regarding umbilical cord stem cell banking. The mean knowledge score was found to be 16.5±6.16. The results also indicated knowledge of staff nurses was found to be significant with years of experience of staff nurses. Hence the study concluded that knowledge of staff nurses were influenced by years’ experience

A Study to Assess the Effectiveness of Self Instructional Module on Knowledge Regarding Stem Cell Banking among Staff Nurses in Selected Hospitals at Bangalore

Aim: Present study attempts to assess the “Effectiveness of self-instructional module on knowledge regarding stem cell banking among staff nurses in hospitals. Methods and Material: Purposive sampling technique with the pre-experimental and post-test design was used. The sample of this study comprised 60 staff nurses who were working in the Obstetrics and Gynaecological ward. A structured knowledge questionnaire was used to collect data from the subjects. The collected data was analyzed using descriptive and inferential statistics and interpreted in terms of the objectives and hypothesis of the study. Results: In pre-test knowledge on stem cell banking, out of 60 staff nurses, 13(22%) had inadequate knowledge, and in post-test after implementation of SIM, 53(88%) of subjects had adequate knowledge, and 7(12%) had moderate knowledge. The above results indicate that SIM effectively increased the ability of staff nurses on stem cell banking. The association was found between the knowledge scores of subjects with demographic variables such as gender, area of residence, and years of clinical experience, which was significant at the level of 0.05. It is recommended that a similar study can be conducted on a larger sample to arrive at a generalization. Moreover, nurses and midwives are part of health care in all the stages of our lives. Thus, nurses must be knowledgeable and aware of recent trends in diagnosis, treatment. Education provides means by which nurses can remain up to date with current developments, maintain their competence and meet the standards of nursing practice. Conclusion: The study’s findings reveal that staff nurses' knowledge had enhanced regarding stem cell banking. Key words: Stem cell banking, Knowledge, Staff nurses.

Marketing and Survey Analysis of Stem Cell Banking in Western Maharashtra

Purpose to check the knowledge of general population and doctors from western Maharashtra about the basics, purpose, utility and capability of stem cells banking and future scopes in medical field. Data was collected from three hospitals from Sangali district. For given study one thousand and two hundred individual from different background and different age group participated between 28th October to 4th January. Among these people 64% of general population heard about stem cell and cord blood banking, only 20% people have detailed information of stem cell banking and 70% medical staff have positive approach toward stem cell banking. Majority of people don’t know importance of cells on deadly disease. Lot of people unaware about technical terms and preservation techniques. Doctors have different opinion on use of stem cells on genetic disorder. 30% doctors though still it need more research and development for use of medical purpose. Hospitals, Institute and young generation need to take action on awareness of stem cell banking. obstetricians and the pediatricians should take a central role in providing the correct information to would be parents to help them in taking a correct decision.

Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation into Azoospermic Torsion Mouse Model

Preserving the spermatogonial stem cells (SSCs) in long periods of time during the treatment of male infertility using stem cell banking systems and transplantation is an important issue. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using 10 mM pentoxifylline (PTX) as an antioxidant in basal freezing medium. Testicular torsion - a mouse model for long-term infertility- was used to transplant fresh SSCs (n = 6), fresh SSCs treated with PTX (n = 6), cryopreserved SSCs with basal freezing medium (n = 6) and cryopreserved SSCs treated with PTX (n = 6). Eight weeks after germ cell transplantation, samples were assessed for proliferation, through evaluation of Ddx4 and Id4 markers, and differentiation via evaluation of C-Kit and Sycp3, Tnp1, Tnp2, and Prm1 markers. According to morphological and flow cytometry results, SSCs are able to form colonies and express Gfra1, ID4, α6‐integrin and β1‐integrin markers. We found positive influence from PTX on proliferative and differentiative markers in SSCs transplanted to azoospermic mice. In the recipient testis, donor SSCs formed spermatogenic colonies and sperm. Respecting these data, adding pentoxifylline is a practical way to precisely cryopreserve germ cells enriched for SSCs in cryopreservation, and this procedure could become an efficient method to restore fertility in a clinical setup. However, more studies are needed to ensure its safety in the long term.

Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model

BackgroundPreserving the spermatogonial stem cells (SSCs) in long periods of time during the treatment of male infertility using stem cell banking systems and transplantation is an important issue. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using 10 mM pentoxifylline (PTX) as an antioxidant in basal freezing medium.MethodsTesticular torsion - a mouse model for long-term infertility- was used to transplant fresh SSCs (n=6), fresh SSCs treated with PTX (n=6), cryopreserved SSCs with basal freezing medium (n=6) and cryopreserved SSCs treated with PTX (n=6). Eight weeks after germ cell transplantation, samples were assessed for proliferation, through evaluation of Ddx4 and Id4 markers, and differentiation via evaluation of C‐Kit and Sycp3, Tnp1, Tnp2, and Prm1 markers.ResultsAccording to morphological and flow cytometry results, SSCs are able to form colonies and express Gfra1, ID4, α6‐integrin and β1‐integrin markers. We found positive influence from PTX on proliferative and differentiative markers in SSCs transplanted to azoospermic mice. In the recipient testis, donor SSCs formed spermatogenic colonies and sperm. ConclusionRespecting these data, adding pentoxifylline is a practical way to precisely cryopreserve germ cells enriched for SSCs in cryopreservation, and this procedure could become an efficient method to restore fertility in a clinical setup. However, more studies are needed to ensure its safety in the long term.

Dental Stem Cell Banking and Applications of Dental Stem Cells for Regenerative Medicine

Since the identification of mesenchymal stem cells, stem cell biology is a greatly researched field of regenerative medicine and tissue engineering therapies and has become an essential part of dentistry. Mesenchymal stem cells are multipotent stem cells that can differentiate into many cell types. Dental mesenchymal stem cell populations have been identified in dental pulp, human exfoliated deciduous teeth, periodontal ligament, dental follicle of third molars, tooth germ of third molars, gingiva of periodontium, alveolar bone, and apical papilla. Dental stem cells are the most natural, noninvasive source of stem cells that have been identified, and they have gained recent attention due to their accessibility and the associated relatively low cost of integration into regenerative therapy. Long-term preservation of dental stem cells is becoming a popular consideration and mirrors the ideology of banking umbilical cord blood. This review outlines the recent progress in the mesenchymal stem cells used in dentistry as well as some advancements that are being made in preserving dental stem cells for future personalized medicine. The aim of this study was to completely and concisely review the current use of adult dental stem cells specifically oral sources of stem cells, banking of dental stem cells, and applications or uses of dental stem cells specifically in oral regions and in a clinical setting.