Evaluation Of Filter Cake Oil As Antifoam In Yeast Production. New Use For This By-Product Of The Sugarcane Derivatives Industry

Purpuse: Fodder yeast is obtained in an aerobic fermentation process where foaming is a major problem to be solved. In this article, the antifoam property of crude and purified filter cake oil is evaluated in order to use this residual as an alternative to replace the import of commercial antifoam agents and to reduce the production costs of fodder yeastMethod: Knock down test and the comparisons with two commercial antifoam agents were done. Blackstrap molasses medium at 20 and 40 g/L of total reducing sugar was used. All products were studied in their pure form and commercial ones also in dilutions 1:2 and 1:5 v/v. Hansen's solubility parameters (HSPs) to analyze the affinity of each defoamer for yeast were determined.Results: It was obtained the crude and purified filter cake oil showed similar behavior to commercial defoamers with an immediate antifoam effect, removing between 40 and 60% of the initial foam at both sugar concentrations in the first 5 minutes. The regression model showed purified filter cake oil has the greatest knockdown effect (Ca = 55.85 and 74.32) and with greater foam suppression stability the commercial defoamer Quimifoam Máster (Cb = -0.69 and -1.38). Ra values obtained in HSPs test, indicated the affinity of defoamers to the medium. Conclusion: Purified filter cake oil is an effective product for its use as an antifoam with the best knock down effect for both concentrations of sugars in the medium. The determination of HSPs corroborates the effectiveness of this product to suppress foam.

Effect of the Use of Yarrowia lipolytica or Saccharomyces cerevisiae Yeast with a Probiotic in the Diet of Turkey Hens on Growth Performance and Gut Histology

The aim of the study was to determine whether the alternative yeast species Yarrowia lipolytica in turkey feed would have a more beneficial effect on growth performance and intestinal histology than the commonly used species Saccharomyces cerevisiae. An additional objective of the study was to test whether the addition of a probiotic to feed containing Yarrowia lipolytica or Saccharomyces cerevisiae yeast would enhance its effect on growth performance and intestinal histology in turkeys. The experiment was carried out on 480 turkey hens randomly divided into six groups. Birds from the control group (C) and group P were fed standard feeds but group P additionally received a probiotic (0.05%). Groups Y and YP received feed containing Yarrowia lipolytica fodder yeast (3%), and the YP group received also the probiotic (0.05%). Similarly, in groups S and SP, the turkeys received feed with Saccharomyces cerevisiae fodder yeast (3%), and for the SP group the probiotic was added to the feed (0.05%). Yarrowia lipolytica yeast added in the amount of 3% to the turkey feed may be an alternative to the commonly used Saccharomyces cerevisiae yeast, because it improved growth performance, and above all, had a more beneficial effect on intestinal histology. The use of Yarrowia lipolytica alone can be beneficial for growth performance, while the combined use of 3% Yarrowia lipolytica in the feed and a 0.05% addition of a probiotic containing Bacillus licheniformis and Bacillus subtilis has a more beneficial effect on gastrointestinal histology.

Effect of the use of Yarrowia lipolytica and Saccharomyces cerevisiae yeast with a probiotic in the diet of turkeys on their gut microbiota and immunity

An experiment was carried out to determine whether the yeast species Yarrowia lipolytica added to compound feeds for turkeys would have a more beneficial effect on their immunity and gut microflora composition than the commonly used species Saccharomyces cerevisiae. An additional aim of the study was to test whether the addition of a probiotic (Bacillus licheniformis and Bacillus subtilis) to the feed containing Yarrowia lipolytica or Saccharomyces cerevisiae yeast would enhance their effect. The experiment was carried out on growing turkey hens aged 7 to 112 days and randomly divided into six groups (each n = 80). The birds in the control group (C) and group P were fed standard feeds, but group P additionally received a probiotic. Groups Y and YP received the feed containing the Yarrowia lipolytica fodder yeast, and the probiotic for the YP group. Similarly, in groups S and SP, the turkeys received the feed with the Saccharomyces cerevisiae fodder yeast, and the probiotic was additionally added to the feed for the SP group. Yarrowia lipolytica yeast can be an alternative to the commonly used yeast Saccharomyces cerevisiae in turkey feeds. Yarrowia lipolytica favourably influenced the intestinal microbiota (reduced the number of microorganisms – P < 0.001, fungi – P < 0.001, and coliforms – P < 0.001, including E. coli), and stimulated erythropoiesis (increased Hb content – P = 0.049 and RBC count – P = 0.027; increased Ht – P < 0.001) and immune mechanisms (increased the %pc – P = 0.021, NBT value – P = 0.013 and lysozyme content – P = 0.013; decreased IgM concentration – P = 0.049). The combined use of a probiotic with yeast, particularly Yarrowia lipolytica, has a more beneficial effect on the gut microbiota than the use of Yarrowia lipolytica alone. The combined use of a probiotic with a yeast, particularly Yarrowia lipolytica, has a more beneficial effect on the gut microbiota than the use of Yarrowia lipolytica alone.

Analysis of economic and technological factors affecting the efficiency of obtaining ethyl alcohol for chemical and fuel purposes

The market value of alcohol production reaches 500 rubles per 1 decaliter (at a cost of up to 430 rubles per 1 decaliter), which does not allow it to be used effectively for purposes other than alcohol beverages. To successfully implement this ethanoll for the needs of the chemical and fuel industry it is necessary to achieve a market value of up to 300 rubles per 1 decaliter (with a cost of 250 rubles per 1 decaliter). To achieve such indicators, studies were carried out on the production of alcohol from starch-containing secondary raw materials of grain complex processing for gluten, starch, syrups, amino acids and organic acids. Samples of starch milk and wheat bran were used as secondary raw materials. As a result, the ratio of starch milk and bran was selected, which allows obtaining a normative yield of alcohol up to 66.3 decaliter / ton of conventional starch. The resulting bard was similar in terms of indicators from the grain, which makes it possible to treat stillage to dried fodder using the same enrgysave equipment. To increase the value of this food, it was enriched with protein by cultivation of fodder yeast. As a result, a fodder product was obtained, corresponding to GOST R 55301-2012 "Yeast fodder from a cereal bard". Based on the conducted studies, a technology was developed for processing a mixture of starch milk and bran in ethyl alcohol and a protein feed product. The technical and economic analysis of the developed technology demonstrates efficiency of secondary raw material using for production of ethyl alcohol and protein fodder.

Nutritional Value of Yarrowia Lipolytica Yeast and its Effect on Growth Performance Indicators n Piglets

Yarrowia lipolytica yeast can be a valuable component of feed compounds due to its high content of protein and essential amino acids, as well as minerals. The objective of the study was to verify the research hypothesis that Yarrowia lipolytica (YL) yeast, having a more valuable chemical composition than Saccharomyces cerevisiae (SC), can improve growth performance in piglets. An attempt was made to determine what proportion of YL yeast in a compound feed for piglets would produce measurable effects in terms of production and health. The experiment was carried out on 46-dayold piglets receiving experimental mixtures for 39 days. The piglets in the experimental groups were fed the same mixture as the control, but with 3% or 6% fodder yeast of the strain Yarrowia lipolytica A-101 in place of soybean meal. The YL fodder yeast had significantly higher content of alanine (by about 50%), lysine, isoleucine and tryptophan (over 30%), leucine, valine, glycine and glutamic acid (over 20%), and tyrosine (by about 17%) in comparison with SC yeast. Content of crude fat was three times higher than in SC yeast. YL yeast had significantly higher content of Na+, K+, Ca+2, Mn+2, S, Mg+2 and Zn+2 than SC, as well as significantly higher content of vitamins B2 and E. Yarrowia lipolytica fodder yeast introduced to compound feed in the amount of just 3% improved growth performance (an increase in daily weight gain and a decrease in feed conversion per kg weight gain). These results indicate that YL in the amount of 3% in compound feed can be successfully used in the diet of piglets in place of the commonly used SC.