Perlakuan Rizobakteri Pemacu Pertumbuhan Tanaman (RPPT) dengan Beberapa Tingkat Kerapatan Inokulum Rizobakteri Terhadap Viabilitas dan Vigor Benih Cabai Merah Kadaluarsa (Capsicum annuum L.)

Abstrak. Biological seed treatment merupakan salah satu perlakuan benih menggunakan mikroorganisme seperti rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian ini bertujuan untuk mengetahui jenis rizobakteri dan kerapatan inokulum yang dapat meningkatkan viabilitas dan vigor benih cabai kadaluarsa. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama adalah jenis rizobakteri (R) terdiri atas lima taraf yaitu R1: Necercia sp; R2:Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. Faktor kedua adalah kerapatan inokulum rizobakteri terdiri dari tiga taraf yaitu K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. Hasil penelitian ini menunjukkan bahwa perlakuan benih menggunakan rizobakteri jenis Necercia sp dengan kerapatan inokulum 108 cfu/ml nyata meningkatkan vigor benih pada tolok ukur indeks vigor yaitu 40% dan Pseudomonas capacia dengan kerapatan inokulum 109 cfu/ml juga merupakan kombinasi perlakuan terbaik dalam meningkatkan berat kering kecambah normal yaitu 69,33 mg.Treatment Of Plant Growth Promoting Rhizobacteria (PGPR)With Multiple Levels of Rhizobacteria Inoculum Density On Viability and Vigor Of Expired Red Chilli Seeds (Capsicum annuum L.Abstract. Biological seed treatment is one of the seed treatment using microorganisms such as plant growth-promoting rhizobacteria (PGPR). This study aims to determine the type of rhizobacteria and inoculum density that can increase the viability and vigor of expired chili seeds. This research uses Completely Randomized Design (CRD) factorial pattern with 2 factors and 3 replications. The first factor is the type of rhizobacteria (R) consists of five levels, namely R1: Necercia sp; R2: Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. The second factor is the density of rhizobacteria inoculum consisting of three levels namely K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. The results of this study showed that the seed treatment using the Necercia sp-type rizobacteria with 108 cfu/ml inoculum density significantly increased the seed vigor on the vigor index benchmark of 40% and Pseudomonas capacia with 109cfu/ml inoculum density was also the best treatment combination in increasing dry weight normal sprout is 69,33 mg.

Efficacy of bacterial vaccines to prevent respiratory disease in swine: a systematic review and network meta-analysis

A systematic review and network meta-analysis (MA) was conducted to address the question, ‘What is the efficacy of bacterial vaccines to prevent respiratory disease in swine?’ Four electronic databases and the grey literature were searched to identify clinical trials in healthy swine where at least one intervention arm was a commercially available vaccine for one or more bacterial pathogens associated with respiratory disease in swine, including Mycoplasma hyopneumoniae, Actinobacillus pleuropneumonia, Actinobacillus suis, Bordetella bronchiseptica, Pasteurella multocida, Stretococcus suis, Haemophils parasuis, and Mycoplasma hyorhinis. To be eligible, trials had to measure at least one of the following outcomes: incidence of clinical morbidity, mortality, lung lesions, or total antibiotic use. There were 179 eligible trials identified in 146 publications. Network MA was undertaken for morbidity, mortality, and the presence or absence of non-specific lung lesions. However, there was not a sufficient body of research evaluating the same interventions and outcomes to allow a meaningful synthesis of the comparative efficacy of the vaccines. To build this body of research, additional rigor in trial design and analysis, and detailed reporting of trial methods and results are warranted.

Pengaruh Perlakuan Benih Dengan Menggunakan Agens Biokontrol Terhadap Pengendalian Penyakit Rhizoctonia solani Pada Pertumbuhan Bibit Cabai Merah

Abstrak. Perlakuan benih menggunakan rizobakteri sebagai alternatif pengganti penggunaan bahan kimia untuk mengendalikan penyakit tanaman. Penelitian ini bertujuan (1) mempelajari pengaruh beberapa jenis isolat rizobakteri dalam menghambat pertumbuhan Rhizoctonia solani terhadap pertumbuhan bibit tanaman cabai merah, dan (2) pengaruh perlakuan benih menggunakan rizobakteri sebagai pemacu pertumbuhan tanaman. Penelitian ini menggunakan rancangan acak lengkap (RAL) non faktorial. Faktor yang diteliti adalah rizobakteri sebagai agens biokontrol terhadap patogen Rizoctonia solani yang terdiri dari 18 isolat rizobakteri yaitu : Pseudomonas capasia (A1) , Bacillus megaterium (A2), Pseudomonas dimuta (A3), Bacillus bodius (A4), Bacillus laterophorus (A5), Bacillus larvae (A6), Bacillus alvei (A7), Bacillus coagulans (A8), Bacillus firmus (A9), Bacillus pilymixa (A10), Bacillus lichiniformis (A11), Bacillus stearothermophillus (A12), Actinobacillus suis (A13), Actinotorbacter sp. (A14), Azotobacter sp. INA8 (A15), Azotobacter sp. (A16), Necercia sp. (A17), Flavobacterium sp. (A18). Sebagai kontrol (A0) juga dikecambahkan benih yang tidak diberi isolat rizobakteri. Terdapat 6 rizobakteri dari 18 isolat yang diuji yang memberikan penurunan nilai gejala serangan patogen Rhizoctonia solani yaitu Pseudomonas dimuta, Bacillus bodius, Bacillus laterophorus, Bacillus lichiniformis, Bacillus stearothermophillus, dan Azotobacter sp. Perlakuan benih dengan rizobakteri Flavobacterium sp sangat nyata meningkatkan vigor benih pada tolok ukur indeks vigor. Perlakuan benih terbaik dalam meningkatkan pertumbuhan tanaman terhadap parameter tinggi tanaman dan diameter batang umur 4 MSP adalah Bacillus bodius dan parameter jumlah daun pada perlakuan jenis rizobakteri Pseudomonas capasia. Influence of Seed Treatment Using Agens Bio-Control Against Rhizoctonia solani Disease and Seedling Growth of Red Chili Pepper Abstract. Seed treatment using rizobakteri as an alternative to substitute the use of chemicals to control plant disease.. This study aims to (1) study the influence of several types of rizobacterial isolates in inhibiting the growth of Rhizoctonia solani on the growth of red chili seedlings, and (2) the influence of seed treatment using rhizobacteria as a trigger for plant growth. This study used a complete randomized design (RAL) non factorial. The factors studied were rizobacteria as a biocontrol agent against Rizoctonia solani pathogen consisting of 18 rizobacterial isolates: Pseudomonas capacia (A1), Bacillus megaterium (A2), Pseudomonas dimuta (A3), Bacillus bodius (A4), Bacillus laterophorus (A5) Bacillus larvae (A6), Bacillus alvei (A7), Bacillus coagulans (A8), Bacillus firmus (A9), Bacillus pilymixa (A10), Bacillus lichiniformis (A11), Bacillus stearothermophillus (A12), Actinobacillus suis (A13), Actinotorbacter sp . (A14), Azotobacter sp. INA8 (A15), Azotobacter sp. (A16), Necercia sp. (A17), Flavobacterium sp. (A18). As control (A0) also added seeds that were not given rizobacterial isolates. There were 6 rhizobacteria of 18 isolates tested which decreased the symptom value of pathogenic attack of Rhizoctonia solani ie Pseudomonas dimuta, Bacillus bodius, Bacillus laterophorus, Bacillus lichiniformis, Bacillus stearothermophillus, and Azotobacter sp. Treatment of seeds with rizobacteria Flavobacterium sp very significantly increases the vigor of seeds on the benchmark vigor index. The best seed treatment in improving plant growth on plant height parameters and stem diameter of 4 MSP was Bacillus bodius and leaf number parameters in the treatment of rhizobacteria Pseudomonas capacia.

Attachment of Actinobacillus suis H91-0380 and Its Isogenic Adhesin Mutants to Extracellular Matrix Components of the Tonsils of the Soft Palate of Swine

Tonsils conduct immune surveillance of antigens entering the upper respiratory tract. Despite their immunological function, they are also sites of persistence and invasion of bacterial pathogens.Actinobacillus suisis a common resident of the tonsils of the soft palate in pigs, but under certain circumstances it can invade, causing septicemia and related sequelae. Twenty-four putative adhesins are predicted in theA. suisgenome, but to date, little is known about how they might participate in colonization or invasion. To better understand these processes, swine tonsil lysates were characterized by mass spectrometry. Fifty-nine extracellular matrix (ECM) proteins were identified, including small leucine-rich proteoglycans, integrins, and other cell surface receptors. Additionally, attachment of the wild type and 3 adhesin mutants to 5 ECM components was evaluated. Exponential cultures of wild-typeA. suisadhered significantly more than stationary cultures to all ECM components studied except collagen I. During exponential growth, theA. suisΔflp1mutant attached less to collagen IV while the ΔompAmutant attached less to all ECMs. The ΔcomE1strain attached less to collagen IV, fibronectin, and vitronectin during exponential growth and exhibited differential attachment to collagen I over short adherence time points. These results suggest that Flp1, OmpA, and ComE1 are important during early stages of attachment to ECM components found in tonsils, which supports the notion that other adhesins have compensatory effects during later stages of attachment.

First Human Case of Meningitis and Sepsis in a Child Caused by Actinobacillus suis or Actinobacillus equuli

We report the first human case of meningitis and sepsis caused in a child byActinobacillus suisorA. equuli, a common opportunistic pathogen of swine or horses, respectively. Identification was performed by matrix-assisted laser desorption ionization–time of flight mass spectrometry and real-time PCR assay. A previous visit to a farm was suspected as the source of infection.