Oxidative Modification of LHC II Associated with Photosystem II and PS I-LHC I-LHC II Membranes

Under aerobic conditions the production of Reactive Oxygen Species (ROS) by electron transport chains is unavoidable, and occurs in both autotrophic and heterotrophic organisms. In photosynthetic organisms both Photosystem II (PS II) and Photosystem I (PS I), in addition to the cytochrome b6/f complex, are demonstrated sources of ROS. All of these membrane protein complexes exhibit oxidative damage when isolated from field-grown plant material. An additional possible source of ROS in PS I and PS II is the distal, chlorophyll-containing light-harvesting array LHC II, which is present in both photosystems. These serve as possible sources of 1O2 produced by the interaction of 3O2 with 3chl* produced by intersystem crossing. We have hypothesized that amino acid residues close to the sites of ROS generation will be more susceptible to oxidative modification than distant residues. In this study, we have identified oxidized amino acid residues in a subset of the spinach LHC II proteins (Lhcb1 and Lhcb2) that were associated with either PS II membranes (i.e. BBYs) or PS I-LHC I-LHC II membranes, both of which were isolated from field-grown spinach. We identified oxidatively modified residues by high-resolution tandem mass spectrometry. Interestingly, two different patterns of oxidative modification were evident for the Lhcb1 and Lhcb2 proteins from these different sources. In the LHC II associated with PS II membranes, oxidized residues were identified to be located on the stromal surface of Lhcb1 and, to a much lesser extent, Lhcb2. Relatively few oxidized residues were identified as buried in the hydrophobic core of these proteins. The LHC II associated with PS I-LHC I-LHC II membranes, however, exhibited fewer surface-oxidized residues but, rather a large number of oxidative modifications buried in the hydrophobic core regions of both Lhcb1 and Lhcb2, adjacent to the chlorophyll prosthetic groups. These results appear to indicate that ROS, specifically 1O2, can modify the Lhcb proteins associated with both photosystems and that the LHC II associated with PS II membranes represent a different population from the LHC II associated with PS I-LHC I-LHC II membranes.

Particle physics today, tomorrow and beyond

The most important discovery in particle physics in recent years was that of the Higgs boson, and much effort is continuing to measure its properties, which agree obstinately with the Standard Model, so far. However, there are many reasons to expect physics beyond the Standard Model, motivated by the stability of the electroweak vacuum, the existence of dark matter and the origin of the visible matter in the Universe, neutrino physics, the hierarchy of mass scales in physics, cosmological inflation and the need for a quantum theory for gravity. Most of these issues are being addressed by the experiments during Run 2 of the LHC, and supersymmetry could help resolve many of them. In addition to the prospects for the LHC, I also review briefly those for direct searches for dark matter and possible future colliders.

Searching for radiative neutrino mass generation at the LHC

In this talk (talk given at the International Conference on Massive Neutrinos, Singapore, 9-13 February 2015), I describe the general characteristics of radiative neutrino mass models that can be probed at the LHC. I then cover the specific constraints on a new, explicit model of this type.