Development of “NEC-SD-LAMP,” a New Non-Electrified Infectious Diseases Testing Method

Here, non-electric-control SalivaDirect loop-mediated isothermal amplification (NEC-SD-LAMP), which can detect infections by analyzing viral gene expression in saliva without electrical control systems, was developed. In this method, viral genes are purified from saliva using SalivaDirect, and gene expression is analyzed by loop-mediated isothermal amplification (LAMP) by adding water to the device, and the results can be visually confirmed. Melting palmitic acid maintains the optimal temperature for the LAMP reaction, as the temperature of palmitic acid is maintained at 62.9°C, its melting point. By taking advantage of the proximity of this melting point to the optimal temperature for LAMP, it is possible to perform LAMP without electricity. Using this method, adenovirus DNA was detected in saliva. Furthermore, the detection limit was 2 copies per µL, indicating that it is possible to detect viral infections in saliva even before the onset of SARS-CoV-2 infection.

A Comprehensive Study on Safety of New Energy Vehicles

New energy vehicles (NEVs) have become a fundamental part of transportation system. Performance of an NEV is hugely determined by batteries, motors, and embedded electric control units. In this paper, a comprehensive study that covers all these key components is presented. Mechanisms and characterizations of failures are given in detail. On top of these, algorithms for fault diagnosis are established based on big data of real-world NEVs with joint considerations of design flaws, usage behaviors, and environmental conditions. In this way, multiple types of faults can be detected ahead of time to avoid accident. Proposed methods have been verified by real-world operational data, indicating effectiveness while providing insights for NEV design optimization.

Design and research on intelligent ventilation system of ship cargo hold

Ventilation in the cargo hold of ships, which can adjust the temperature and humidity in the cargo hold, is a necessary measure to ensure the safety of maritime cargo. Traditional ship cargo hold ventilation includes natural ventilation and mechanical ventilation. Its ventilation effect is limited, which often leads to cargo damage accidents such as mildew of the cargo in the cabin. Based on the research of intelligent cargo hold ventilation technology, this paper proposes a design scheme of ship cargo hold ventilation system. The ventilation system includes a system platform and electrical connection elements supported by the system platform. Among them, the system platform is electrically connected to the concentrator, the concentrator is electrically connected to several collectors and electric control devices, the collector is electrically connected to several collection nodes, and the electric control device is electrically connected to the ventilation device. The collection nodes of the ventilation system, including temperature and humidity sensors, air pressure sensors, and wind speed and direction sensors, are installed in the cargo hold of the ship. The collection nodes are evenly distributed in multiple locations in the cargo hold of the ship, effectively monitoring the temperature, humidity, air pressure, wind speed and direction of the cargo hold of the ship, and transmitting it to the system platform through collectors and concentrators to realize real-time monitoring. According to the setting of different environmental standard values, it can automatically complete the startup, shutdown and reverse operation of the wind turbines in different positions, making the system more accurate in monitoring the environment of the ship’s cargo hold, effectively reducing cargo damage, ensuring the safety and economy of maritime freight, and It can more effectively save energy, reduce carbon emissions, protect the environment, and make the system more practical.