In Vivo Protein Profiling & Estimation of Plant Secondary Metabolites, Phylogenetic Analysis of Plant Leaves for Antifungal Activity

The present research work involved to evaluated the phytochemical screening on different plant leaf extracts, protein profiling and phylogenetic analysis of leaf extracts which is Psidium guajava, Syzygium cumini, Aegle marmelos, Nyctanthes arbor-tristis, Callistemon lanceolatus, Citrus limonum, Combretum indicum, Calliandra haematocephala, Polyalthia longifolia were used fresh leaves of the plants and analysed the phylogenetic analysis between all plant leaf which having antifungal activity against plant pathogens .According to study it was found that several plant leaf contain different bands of different molecular weight which is Nyctanthes arbortristis 80KDa, 72KDa ,70KDa, 63KDa, 61KDa, 53KDa, 50KDa, 41KDa, 38KDa, 37KDa. Aegle marmelos 72KDa, 70KDa, 64KDa, 54KDa, 54KDa, 40KDa, 36KDa, 35KDa. Polyalthia longifolia 75KDa, 70KDa, 44KDa, 41KDa, 35KDa. Psidium guajava 75KDa, 70KDa, 54KDa, 53KDa, 45KDa, 40KDa, 35KDa.The protein profiling of the different leaf extracts Psidium guajava, Syzygium cumini, Aegle marmelos, Nyctanthes arbor-tristis, Callistemon lanceolatus, Citrus limonum, Combretum indicum, and Calliandra haematocephala, Polyalthia longifolia gave an idea that proteins and peptides present in the leaves. The proteins also may act as antifungal agents. Thus, after the protein profiling was done, based on this the UPGMA (Un-weighted paired group method with arithmetic average) software was used to identify the evolutionary presence of plant. The evolutionary relationship has thus been depicted between the different trees.

Evaluation of anti-dermatophytic activity of medicinal plants derived products

<p class="abstract"><strong>Background: </strong>Dermatophytes are a group of closely related fungi which are able to invade the keratinized tissue skin, hair and nail. In this study different medicinal plants like <em>Melaleuca alternifolia, Zingiber officinale, Allium sativum, Azadirachta indica, Citrus limonum, Curcuma longa, Cocos nucifera</em> were used as antifungal agent against different dermatophyte species.</p><p class="abstract"><strong>Methods: </strong>A hospital-based study consisting of 320 patients clinically diagnosed having dermatophytic infection who reported to the Dermatology outpatient department (OPD), Adesh medical college, Bathinda was conducted for the period of 2 years. Socio-demographic and clinical information was collected and sample was taken from the edge of infected area which was then collected in 2 ml of Eppendorf. Extracts of medicinal plants were then explored against dermatophyte. The data was evaluated using appropriate statistical method.</p><p class="abstract"><strong>Results: </strong>All dermatophyte species were found sensitive for <em>Melaleuca alternifolia, Zingiber officinale and Allium sativum</em>. These medicinal plants showed very good results as antifungal against dermatophytes while <em>Azadirachta indica </em>and <em>Citrus limonum </em>were moderate sensitive and <em>Curcuma longa </em>and <em>Cocos nucifera </em>did not show any zone of inhibition around the well.</p><p class="abstract"><strong>Conclusions: </strong>This research provides a scientific validation for the use of these medicinal plants in the treatment of dermatophytic infection and could be used in future for dermatophytic infection and other skin infection.</p>

A Systematic Overview on Some of the Traditionally Used Plants of Assam

Assam is a state of the North Eastern part of India. There are different types of plants well distributed in various parts of the state. These plants have various medicinal properties and are used traditionally to cure diseases because of its fewer side effects. A large number of surveys have been documented about different kinds of traditionally used plants of Assam. The purpose of this Study is to do a Systematic overview on various aspects like its taxonomical characters, geographical habitat, Cultivation along with its physicochemical constituents, Pharmacological and medicinal properties so that it can be useful to treat the various diseases efficiently. Various traditional plants such as Carica papaya, Cocos nucifera, Murraya koenigii, Ocimum sanctum, Musa paradisiaca, Averrhoa carambola, Aegle marmelos, Azadirachta indica, Citrus limonum, Psidium guajav, Hibiscus rosa sinensis, Catharanthus roseus, Trichosanthes dioica, Cynodon dactylon, Brassica rapa were reviewd. Keywords: Assam, plants, Physicochemical constituents, Pharmacological and Medicinal uses.

Uso de fitoterápicos na cura de enfermidades em animais no Semiárido Paraibano

O uso de plantas medicinais mostra-se como uma alternativa, para o tratamento e prevenção de doenças que acometem os animais, visto que, é um método de baixo custo e de fácil aquisição ao produtor. Desta forma, objetivou-se avaliar o conhecimento populacional no semiárido paraibano, a respeito do emprego de plantas com propriedades curativas para animais. A pesquisa foi realizada na 14ª Região Administrativa da Empresa de Assistência Técnica e Extensão Rural da Paraíba, sediada em Pombal-PB, promovendo-se uma pesquisa de campo, de caráter descritivo com uma abordagem qualitativa. Como instrumento para coleta de dados utilizou-se um questionário previamente estruturado, contendo questões objetivas e subjetivas. Sendo observado entre os entrevistados que a planta medicinal mais utilizada para a cura de enfermidades em animais foi o limoeiro (Citrus limonum ) com 12,14%, seguido pelo Fedegoso ( Senna ocidental ) com 10,57%, feijão bravo ( Capparis flexuosa ) equivalente a 9,57% e por baba ( Aloe vera ) com 8,14%. Entre as famílias que mais se destacaram, estão Anacardiaceae, Fabaceae e Euphorbiaceae, com espécies endêmicas da Caatinga.