Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda

This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center. Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.

Nonsense mutation in CFAP43 causes normal-pressure hydrocephalus with ciliary abnormalities

ObjectiveTo identify genes related to normal-pressure hydrocephalus (NPH) in one Japanese family with several members with NPH.MethodsWe performed whole-exome sequencing (WES) on a Japanese family with multiple individuals with NPH and identified a candidate gene. Then we generated knockout mouse using CRISPR/Cas9 to confirm the effect of the candidate gene on the pathogenesis of hydrocephalus.ResultsIn WES, we identified a loss-of-function variant in CFAP43 that segregated with the disease. CFAP43 encoding cilia- and flagella-associated protein is preferentially expressed in the testis. Recent studies have revealed that mutations in this gene cause male infertility owing to morphologic abnormalities of sperm flagella. We knocked out mouse ortholog Cfap43 using CRISPR/Cas9 technology, resulting in Cfap43-deficient mice that exhibited a hydrocephalus phenotype with morphologic abnormality of motile cilia.ConclusionOur results strongly suggest that CFAP43 is responsible for morphologic or movement abnormalities of cilia in the brain that result in NPH.

Recurrent supraventricular tachycardia and necrotizing enterocolitis: A causative role or a simple association? A case report and literature review

Insufficiency in mesenteric flow is a risk factor for the development of necrotizing enterocolitis (NEC). Recurrent episodes of supraventricular tachycardia (SVT) can lead to gut ischemia, with subsequent reperfusion injury. We present a term infant who developed NEC at 37 days of life after refractory SVT and reverted to sinus rhythm on day 23 and 25 of life. Resected stenotic ileum and transverse colon demonstrated inflammation with ischemia. This is the first case of NEC following SVT in a term infant without a congenital morphologic abnormality. In view of the temporal sequence of events, and in the absence of other risk factors for NEC, the SVT and NEC were likely causally related.

The white IRIS leukocyte differential analyzer for rapid high-precision differentials based on images of cytoprobe-reacted cells

Combining unique cytoprobe, rapid hemacyte fractionation, and novel color image analysis, The White IRIS (TWI) extends automated intelligent microscopy to leukocyte differentiation. TWI provides flow cytometry precision and microscopical resolution to review specimens flagged by hematology analyzers with differential capabilities or to complement other analyzers without these capabilities. The system includes compartments for closed sampling, rapid leukocyte-rich plasma preparation, cytoprobe-induced metachromasia, and collection and color analysis of leukocyte images, and presents the results as a single-view 500-cell differential on a 20-in. (50-cm) touch-screen monitor. Method correlations for the five mature cell types averaging r &gt; 0.90 were obtained with a prototype system. Classification of normal and abnormal specimens showed 95% agreement with a reference method without any undetected significant morphologic abnormality. False-positive and false-negative rates of 7.27% and 3.53%, respectively, exceeded performance of current commercial systems. Case studies demonstrate the ease and speed with which unusual pathologies and leukemias can be observed and interpreted.

Atypical Familial Hemolytic Anemia

1. The genetic, clinical and hematologic features of an atypical chronic hemolytic anemia in two siblings of a French Canadian family have been described. 2. The anemia is normocytic, normochromic and not associated with any characteristic morphologic abnormality of the red cells. 3. Slight increases in osmotic and incubated mechanical fragility, as well as a more definite increase in aumtohemolysis were found which could not be demonstrated after splenectomy. 4. The survival time of normal red cells was shortened before splenectomy in one patient. Normal red cell survival was demonstrated in both patients after splenectomy. 5. The features which differentiate this hemolytic anemia from hereditary spherocytosis are discussed. 6. French or French Canadian ancestry has been noted in some of the reported patients most similar to our own. 7. The association of this type of hemolytic anemia with blood group A has been confirmed in our patients. 8. Splenectomy decreased the severity of the hemolytic process in both patients. This benefit may have resulted from removal of an extracorpuscuar hemolytic mechanism.