Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures

Microalgal biotechnology shows considerable promise as a sustainable contributor to a broad range of industrial avenues. The field is however limited by processing methods that have commonly hindered the progress of high throughput screening, and consequently development of improved microalgal strains. We tested various microplate reader and flow cytometer methods for monitoring the commercially relevant pigment fucoxanthin in the marine diatom Phaeodactylum tricornutum. Based on accuracy and flexibility, we chose one described previously to adapt to live culture samples using a microplate reader and achieved a high correlation to HPLC (R2 = 0.849), effectively removing the need for solvent extraction. This was achieved by using new absorbance spectra inputs, reducing the detectable pigment library and changing pathlength values for the spectral deconvolution method in microplate reader format. Adaptation to 384-well microplates and removal of the need to equalize cultures by density further increased the screening rate. This work is of primary interest to projects requiring detection of biological pigments, and could theoretically be extended to other organisms and pigments of interest, improving the viability of microalgae biotechnology as a contributor to sustainable industry.

The experience of using Arthrospira platensis as a feed additive in the cultivation of juveniles of the Far Eastern sea cucumber (Apostichopus japonicus)

The experience of using the culture of Arthrospira (Spirulina) platensis as an additive to feed when growing juveniles of the Far Eastern sea cucumber in aquarium conditions is described. The experimental food ration included live culture of spirulina, macroalgae, protein and stimulant supplements. There were no significant differences in the survival rates of individuals (experiment – 69.5%, control – 65.7%). The greatest increase in the mass of individuals was observed in the experimental container - by an average of 0.047 g. In the control group - an average of 0.009 g.

Reconstructing the everyday culture of medieval society in the museum exposition

The issue of representing the everyday culture of archaic societies in the museum exposition is quite relevant for modern museology. The culture of everyday life itself plays an important role in studying history of culture as well as the existence of society in different historical periods; therefore its museum interpretation requires particular emphasis. The author reflects on things, which distinguishes historical, long-defunct everyday live culture, artificially created in the exposition of the open-air museum, from the modern, original one, which is being saved by various ecomuseums. In addition, the article identifies three forms of expression of everyday culture, examines the possibilities of its reconstruction, relationship with each other and with the museum itself in the process of creating a general picture of medieval society’s life. Thus, the reconstruction of everyday culture appears to be a strictly historical phenomenon, which is impossible without theatricalization and other creative elements

Novel Spiroplasma sp. Isolated From CWD Is an Extreme Bacterial Thermoacidophile That Survives Autoclaving, Boiling, Formalin Treatment, and Significant Gamma Irradiation

Rapid spreading of chronic wasting disease (CWD) in wildlife and captive cervid populations has exposed lack of progress in dealing with the transmissible spongiform encephalopathies (TSE) of man and animals. Since the TSE transmissible agent was resistant to extremes in environmental and chemical treatments, focus was on an unconventional agent including the prion theory. Recent breakthrough research has revealed consistent isolation of a novel Spiroplasma sp. from TSE-affected tissues that propagates in cell-free media and on agar. Here, we developed a live culture assay to test whether the CWD spiroplasma isolate possessed unconventional biologic properties akin to those of the transmissible agent of TSE. The CWD spiroplasma isolate survived boiling for 1 hour, standard liquid autoclaving, 10% formalin treatment overnight, and gamma irradiation of 20 kGy. The CWD spiroplasma isolate is an acidophile, growing best at pH 2. The biologic resistance of the CWD spiroplasma isolate may be due to unusual phage-like viruses found in the bacterial pellet or to DNA-protein binding. Because the CWD spiroplasma isolate has biologic properties consistent with the causal agent of the TSEs, TSE research focus should be redirected to development of diagnostic tests and preventive vaccines for control of CWD based upon the bacterium.

Penguatan Pendidikan Karakter Siswa Melalui Manajemen Bimbingan dan Konseling Islam

The importance of character education is a wisdom of value diversity and social live culture. To achive the goal, the education system in school has developed three sub systems,namely, administration, instruction, and pupil/ student personal service. Guidance andcounseling program is from pupil/ student personal service. Strengthening charactereducation can be realized through the management of Islamic guidance and counseling.According to management concept, implementation of guidance and counselingmanagement isone of the manivestation of systematic program on how to plan guidanceand counseling activity, how to lead human resource in the organization in order toachieve the goal, how to control the activity of guidance and counseling, andhow toevaluate it. Therefore, the management of Islamic guidance and counselingcan achievethe education goal exspecially in strengthening education character.

The effect of Saccharomyces cerevisiae on in vitro growth and fermentation of Selenomonas ruminantium and Megasphaera elsdenii

Stimulation of lactate utilization by Selenomonas ruminantium and Megasphaera elsdenii may help in reducing problems associated with rumen acidosis. The objective of this study was to determine the effect of a Saccharomyces cerevisiae live culture and Saccharomyces cerevisiae fermentation products on in vitro growth and fermentation of lactate-utilizing ruminal bacteria, S. ruminantium (ATCC 19205) and M. elsdenii (ATCC 25940). The cultures were run for 0, 6, 12, 24 and 48 h under anaerobic conditions on a growth medium supplemented with a yeast live culture (SC) or with yeast fermentation products (SCFP) and, as reference, on the same medium without supplementation (CON). Neither SC nor SCFP had a significant effect on the growth of S. ruminantium after 6, 12 and 24 h of incubation, but the live yeast culture significantly (P≤0.05) improved the growth of these bacteria after 48 h of incubation. The yeast fermentation products significantly (P≤0.05) decreased pH and increased lactate synthesis by S. ruminantium. The Saccharomyces cerevisiae live culture significantly improved the growth of M. elsdenii after 12 and 24 h of incubation, and the S. cerevisiae fermentation products increased its growth after 48 h. The After 24 and 48 h of incubation the Saccharomyces cerevisiae live culture reduced the concentration of total volatile fatty acids (VFA), while caproate was the main product of in vitro fermentation of M. elsdenii (P≤0.05). Saccharomyces cerevisiae live cultures may improve microbial fibre fermentation in the rumen by maintaining optimal pH conditions.