Zero-Overlap Fluorophores for Fluorescent Studies at Any Concentration

2020 ◽  
Vol 142 (28) ◽  
pp. 12167-12180 ◽  
Author(s):  
Ayan Dhara ◽  
Tumpa Sadhukhan ◽  
Edward G. Sheetz ◽  
Andrew H. Olsson ◽  
Krishnan Raghavachari ◽  
...  
Keyword(s):  
Fluorescent Studies

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

scholarly journals Isolation of RNA from a mixture and its detection by utilizing a microgel-based optical device

2018 ◽  
Vol 96 (12) ◽  
pp. 1079-1086 ◽  
Author(s):  
Molla R. Islam ◽  
Shakiba Azimi ◽  
Faranak Teimoory ◽  
Glen Loppnow ◽  
Michael J. Serpe
Keyword(s):  
Melting Temperature ◽  
Magnetic Beads ◽  
Colorimetric Detection ◽  
Optical Devices ◽  
High Melting ◽  
Dna Duplex ◽  
Dna And Rna ◽  
High Melting Temperature ◽  
Hybrid Duplex ◽  
Fluorescent Studies

In this investigation, we show that RNA can be separated from a solution containing DNA and RNA and the isolated RNA can be detected using poly (N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) microgel-based optical devices (etalons). The isolation of RNA was accomplished by using hairpin-functionalized magnetic beads (MMPDNA) and differential melting, based on the fact that the DNA–RNA hybrid duplex is stronger (i.e., high melting temperature) than the DNA–DNA duplex (i.e., low melting temperature). By performing concurrent etalon sensing and fluorescent studies, we found that the MMPDNA combined with differential melting was capable of selectively separating RNA from DNA. This selective separation and simple colorimetric detection of RNA from a mixture will help lead to future RNA-based disease diagnostic devices.

Fluorescent studies on the interaction between a novel Ca2+ antagonist, SR 33557, and membrane lipids

1992 ◽  
Vol 43 (7) ◽  
pp. 1513-1520 ◽  
Author(s):  
P. Chatelain ◽  
J.R. Matteazzi ◽  
R. Laruel
Keyword(s):  
Membrane Lipids ◽  
Fluorescent Studies

Fluorescent studies on the interaction of DNA and ternary lanthanide complexes with cinnamic acid-phenanthroline and antibacterial activities testing

Luminescence ◽  
2014 ◽  
Vol 30 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Hui-Juan Sun ◽  
Ai-Ling Wang ◽  
Hai-Bin Chu ◽  
Yong-Liang Zhao
Keyword(s):  
Cinnamic Acid ◽  
Lanthanide Complexes ◽  
Antibacterial Activities ◽  
Fluorescent Studies

Phosphotungstic Acid: An Efficient Catalyst for Synthesis of 2-Substituted Tetrahydroquinoline via Imino Diels–Alder Reaction and Fluorescent Studies

2010 ◽  
Vol 41 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Kavitha C. S. Achar ◽  
Kallappa M. Hosamani ◽  
Harisha R. Seetharamareddy
Keyword(s):  
Phosphotungstic Acid ◽  
Alder Reaction ◽  
Diels Alder ◽  
Efficient Catalyst ◽  
Diels Alder Reaction ◽  
Fluorescent Studies

scholarly journals INCLUSION STUDIES ON α-CYCLODEXTRIN COMPLEXES OF GLIPIZIDE AND GLICLAZIDE WITH EFFECT OF PH

2016 ◽  
Vol 10 (1) ◽  
pp. 273 ◽  
Author(s):  
Lizy Roselet Simon ◽  
Prema Kumari J
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Inclusion Complexes ◽  
Oral Bioavailability ◽  
Spectroscopic Studies ◽  
Liquid Inclusion ◽  
Oral Antidiabetic Drugs ◽  
Cyclodextrin Complexes ◽  
Oral Antidiabetic ◽  
Vis Spectroscopy ◽  
Fluorescent Studies

ABSTRACTObjective: To synthesize inclusion complexes of oral antidiabetic drugs, i.e., Glipizide (GP) and Gliclazide (Glc) with a-cyclodextrin (α-CD) and varyingthe pH which will enhance the solubility and thereby oral bioavailability of the drugs.Methods: Liquid inclusion complexes were prepared and characterized by ultraviolet-visible (UV-VIS) spectroscopic studies, and fluorescent studiessolid inclusion complexes of GP and Glc with α-CD were prepared by co-evaporation method and characterized by differential scanning calorimetricstudies (DSC) and1H nuclear magnetic resonance spectroscopic studies (1H NMR).Results: The inclusion complexes of both GP and Glc with α-CD showed high binding constant values for pH 2 from the UV-VIS spectroscopy studiesand fluorescent studies. DSC and1H NMR spectroscopic studies confirmed the inclusion of GP and Glc inside the α-CD cavity.Conclusion: Formed with more stable inclusion complexes, with α-CD are GP and Glc at pH 2, along with pH modulation could be a suitable strategyto enhance the solubility and oral bioavailability of both the drugs.Keywords: Solubility, Bioavailability, Glipizide, Gliclazide, Inclusion complexes, α-Cyclodextrin.

scholarly journals From gold nanoparticles to luminescent nano-objects: experimental aspects for better gold-chromophore interactions

Nanophotonics ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 71-92 ◽  
Author(s):  
Julien R.G. Navarro ◽  
Frederic Lerouge
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Quenching ◽  
Radiative Decay ◽  
Decay Rates ◽  
Gold Nanostructures ◽  
Hybrid Particles ◽  
Enhanced Fluorescence ◽  
Anisotropic Nanoparticles ◽  
Complex Shapes ◽  
Fluorescent Studies

AbstractGold nanoparticles have been the center of interest for scientists since many decades. Within the last 20 years, the research in that field has soared with the possibility to design and study nanoparticles with controlled shapes. From spheres to more complex shapes such as stars, or anisotropic architectures like rods or bipyramids, these new systems feature plasmonic properties making them the tools of choice for studies on light-matter interactions. In that context, fluorescence quenching and enhancement by gold nanostructures is a growing field of research. In this review, we report a non-exhaustive summary of the synthetic modes for various shapes and sizes of isotropic and anisotropic nanoparticles. We then focus on fluorescent studies of these gold nano-objects, either considering “bare” particles (without modifications) or hybrid particles (surface interaction with a chromophore). In the latter case, the well-known metal-enhanced fluorescence (MEF) is more particularly developed; the mechanisms of MEF are discussed in terms of the additional radiative and non-radiative decay rates caused by several parameters such as the vicinity of the chromophore to the metal or the size and shape of the nanostructures.

Immuno-fluorescent studies with hookworms I. Antigenic relationships of ova

1965 ◽  
Vol 59 (6) ◽  
pp. 690-693 ◽  
Author(s):  
V. Zaman ◽  
Mulkit Singh
Keyword(s):  
Antigenic Relationships ◽  
Fluorescent Studies
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