Importance: Consensus has not been reached regarding the prognostic value of various pathological factors, including extranodal extension (ENE), in patients with head and neck squamous cell carcinoma (HNSCC). Objective: To evaluate associations of pathological findings in neck dissection on survival rates of HNSCC Design: A retrospective study of patients with HNSCC treated during 2009-2017, either surgically (Surgery Group) or with radiotherapy or chemotherapy, followed by neck dissection (Organ Preservation Group). The respective mean follow-up periods were 50.1±44.1 and 43.3±27.4 months. Setting: A tertiary care university-affiliated medical Patients: 135 patients were selected consecutively. After excluding 36 with oropharyngeal cancer, nasopharyngeal carcinoma, or an unknown primary, the cohort comprised 72 patients in the Surgery Group and 27 in the Organ Preservation Exposure: Surgical treatment or the combination of radiotherapy/chemoradiotherapy and neck dissection. Main outcomes: Five-year overall survival and associations of demographic and nodal Characteristics with Results: The Surgery Group comprised 53/72 males, mean age 71.6±12.96 years. The Organ Preservation Group comprised 26/27 males, mean age 71.3±10.5 years. For the respective groups, the mean values of nodal yield were 30.54±13.09 and 18±9.33, and the mean numbers of pathological lymph nodes were 2.09±3.7 and 0.88 ± 1.8; ENE was detected in 39% and 22%, respectively. In the Surgery Group, the AJCC 8th edition guidelines upstaged 28/53 patients (53%) with nodal metastases. The 5-year overall survival rate was 44.7%: 31% vs 55% for patients with without ENE (p=0.037). In a multivariate Cox proportional-hazard model, age and the number of pathological nodes were associated with overall survival. In the Organ Preservation Group, the 5-year overall survival rate was 44%. In a multivariate Cox model, ENE and the number of pathological nodes were associated with overall survival. None of the patients with ENE in the last group survived at 5 years. Conclusion: Among patients with HNSCC, the number of positive nodes in neck dissection was associated with overall survival, both in those for whom surgery was first-line treatment and those who underwent surgery following chemoradiotherapy. However, ENE was associated with overall survival in the latter group only, with fatal outcome.