A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole-Cell Bacteria by MALDI-TOF MS

Purpose:We report a case of a 72-year-old man with bleb-related endophthalmitis (BRE) whose vitreous samples were directly analyzed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly identify the causative organism, whereas the results from conventional microbiological techniques were negative.Methods:We analyzed BRE vitreous samples with MALDI-TOF MS (Vitek MS, bioMérieux) for rapid pathogen identification without prior culture. Samples were also analyzed with standard microbiological methods.Results:Within 1 hour of sample acquisition, MALDI-TOF MS identified Gemella sanguinis from the undiluted vitreous sample from vitrectomy without prior culture with a confidence value of 99.7%. Gram stain and cultures from aqueous and vitreous samples were negative for 28 days after acquisition. The patient’s right-eye vision improved from hand motion to 20/50 2 months later.Conclusions:Our findings suggest that the direct analysis of intraocular samples with MALDI-TOF MS could be a novel, promising adjuvant method of rapid endophthalmitis diagnosis.

Download Full-text

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot4618 ◽

2007 ◽

Vol 2007 (8) ◽

pp. pdb.prot4618-pdb.prot4618

Author(s):

L. J. Deterding ◽

J. M. Cutalo ◽

K. B. Tomer

Keyword(s):

Metal Ion ◽

Direct Analysis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

Download Full-text

Principal component analysis of MALDI-TOF MS of whole-cell foodborne pathogenic bacteria

Analytical Biochemistry ◽

10.1016/j.ab.2020.113582 ◽

2020 ◽

Vol 592 ◽

pp. 113582 ◽

Cited By ~ 2

Author(s):

Wenjing Yan ◽

Jing Qian ◽

Yongjie Ge ◽

Keping Ye ◽

Cunshan Zhou ◽

...

Keyword(s):

Principal Component Analysis ◽

Pathogenic Bacteria ◽

Principal Component ◽

Component Analysis ◽

Whole Cell ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Foodborne Pathogenic Bacteria ◽

Tof Ms

Download Full-text

Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis

Journal of Biotechnology ◽

10.1016/j.jbiotec.2013.04.001 ◽

2013 ◽

Vol 165 (3-4) ◽

pp. 167-174 ◽

Cited By ~ 25

Author(s):

Maria Isabel Stets ◽

Artur Soares Pinto ◽

Luciano Fernandes Huergo ◽

Emanuel Maltempi de Souza ◽

Vandeir Francisco Guimarães ◽

...

Keyword(s):

High Resolution ◽

Rapid Identification ◽

Bacterial Isolates ◽

Wheat Roots ◽

Whole Cell ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Ms Analysis ◽

Tof Ms

Download Full-text

Quantitative whole-cell MALDI-TOF MS fingerprints distinguishes human monocyte sub-populations activated by distinct microbial ligands

BMC Biotechnology ◽

10.1186/s12896-015-0140-1 ◽

2015 ◽

Vol 15 (1) ◽

Cited By ~ 16

Author(s):

Damien Portevin ◽

Valentin Pflüger ◽

Patricia Otieno ◽

René Brunisholz ◽

Guido Vogel ◽

...

Keyword(s):

Human Monocyte ◽

Whole Cell ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

Download Full-text

Direct analysis of positive blood cultures using MALDI-TOF MS profiling and a fundamentally improved sample preparation technique

Pathology ◽

10.1016/s0031-3025(16)33250-0 ◽

2011 ◽

Vol 43 ◽

pp. S73-S74

Author(s):

Guido Mix ◽

Leith Fremlin ◽

Thomas Maier ◽

Beatrix Wegemann ◽

Markus Kostrzewa

Keyword(s):

Sample Preparation ◽

Direct Analysis ◽

Blood Cultures ◽

Preparation Technique ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Sample Preparation Technique ◽

Tof Ms

Download Full-text

Identification of the Corn Pathogen Pantoea stewartii by Mass Spectrometry of Whole-Cell Extracts and Its Detection with Novel PCR Primers

Applied and Environmental Microbiology ◽

10.1128/aem.01032-10 ◽

2010 ◽

Vol 76 (18) ◽

pp. 6248-6256 ◽

Cited By ~ 28

Author(s):

Annette Wensing ◽

Stefan Zimmermann ◽

Klaus Geider

Keyword(s):

Mass Spectrometry ◽

Pcr Primers ◽

Enteric Bacteria ◽

Cell Protein ◽

Whole Cell ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Pantoea Stewartii ◽

Ms Analysis ◽

Tof Ms

ABSTRACT Pantoea stewartii subsp. stewartii is the causative agent of Stewart's wilt, a bacterial disease transmitted by the corn flea beetle mainly to sweet corn (Zea mays). In many countries, it is classified as a quarantine organism and must be differentiated from other yellow enteric bacteria frequently occurring with corn. We have created novel primers from the pstS-glmS region of P. stewartii for use in conventional PCR (cPCR) and quantitative PCR (qPCR). To facilitate rapid diagnosis, we applied matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Using whole-cell protein extracts, profiles were generated with a Bruker microflex machine, and the bacteria classified. P. stewartii strains were clearly distinguished from strains of Pantoea agglomerans, Pantoea dispersa, and Pantoea ananatis. Dendrogram analysis of the protein profiles confirmed the score values and showed the formation of separate clades for each species. The identification achieved by MALDI-TOF MS analysis agrees with the diagnosis by specific PCR primers. The combination of both methods allows a rapid and simple identification of the corn pathogen. P. stewartii subsp. stewartii and P. stewartii subsp. indologenes are highly related and can be distinguished not only by virulence assays and indole tests but also by a characteristic pattern in the nucleotide sequence of recA.

Download Full-text

Rapid Identification of Vibrio parahaemolyticus by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Applied and Environmental Microbiology ◽

10.1128/aem.01171-09 ◽

2009 ◽

Vol 75 (21) ◽

pp. 6745-6756 ◽

Cited By ~ 51

Author(s):

Tracy H. Hazen ◽

Robert J. Martinez ◽

Yanfeng Chen ◽

Patricia C. Lafon ◽

Nancy M. Garrett ◽

...

Keyword(s):

Vibrio Parahaemolyticus ◽

Laser Desorption ◽

Rapid Identification ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Whole Cell ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Tof Ms

ABSTRACT Vibrio parahaemolyticus is a pathogenic marine bacterium that is the main causative agent of bacterial seafood-borne gastroenteritis in the United States. An increase in the frequency of V. parahaemolyticus-related infections during the last decade has been attributed to the emergence of an O3:K6 pandemic clone in 1995. The diversity of the O3:K6 pandemic clone and its serovariants has been examined using multiple molecular techniques including multilocus sequence analysis, pulsed-field gel electrophoresis, and group-specific PCR analysis. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a powerful tool for rapidly distinguishing between related bacterial species. In the current study, we demonstrate the development of a whole-cell MALDI-TOF MS method for the distinction of V. parahaemolyticus from other Vibrio spp. We identified 30 peaks that were present only in the spectra of the V. parahaemolyticus strains examined in this study that may be developed as MALDI-TOF MS biomarkers for identification of V. parahaemolyticus. We detected variation in the MALDI-TOF spectra of V. parahaemolyticus strains isolated from different geographical locations and at different times. The MALDI-TOF MS spectra of the V. parahaemolyticus strains examined were distinct from those of the other Vibrio species examined including the closely related V. alginolyticus, V. harveyi, and V. campbellii. The results of this study demonstrate the first use of whole-cell MALDI-TOF MS analysis for the rapid identification of V. parahaemolyticus.

Download Full-text