scholarly journals Sirtuin 6 contributes to migration and invasion of osteosarcoma cells via the ERK1/2/MMP9 pathway

FEBS Open Bio ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 1291-1301 ◽  
Author(s):  
Hang Lin ◽  
Yi Hao ◽  
Zhengxu Zhao ◽  
Yongjun Tong
Keyword(s):  
Migration And Invasion ◽  
Osteosarcoma Cells

scholarly journals Potential Antimetastatic Effect of Timosaponin AIII against Human Osteosarcoma Cells through Regulating the Integrin/FAK/Cofilin Axis

2021 ◽  
Vol 14 (3) ◽  
pp. 260
Author(s):  
Yi-Hsien Hsieh ◽  
Wen-Hung Hsu ◽  
Shun-Fa Yang ◽  
Chung-Jung Liu ◽  
Ko-Hsiu Lu ◽  
...  
Keyword(s):  
Integrin Αvβ3 ◽  
Migration And Invasion ◽  
Steroidal Saponin ◽  
Αvβ3 Integrin ◽  
Osteosarcoma Cells ◽  
Inhibitory Effects ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Timosaponin Aiii

Timosaponin AIII (TSAIII) is a steroidal saponin which demonstrates anti-tumour activities. However, the effect of TSAIII on human osteosarcoma cells remains largely unknown. In this study, we demonstrated that TSAIII exerted a significant inhibitory effect on the distribution of cytoskeletal F-actin and cytoskeletal-related proteins, which contributed to the suppression of cell migration and invasion, without inhibiting cell growth or apoptosis. In the synergistic inhibitory analysis, cotreatment of TSAIII with αVβ3 integrin inhibitor [Cyclo(RGDyK)] or focal adhesion kinase (FAK) inhibitor (PF-573228) exerted greater synergistic inhibitory effects on the expression of Intergin αVβ3/FAK/cofilin axis, thus inhibiting the migration and invasion capacities of human osteosarcoma cells. TSAIII was demonstrated to significantly inhibit the pulmonary metastasis formation of human osteosarcoma cells in vivo in metastasis animal models. These findings reveal the inhibitory effects of TSAIII on the metastasis progression of human osteosarcoma cells and the regulation of integrin-αVβ3-FAK-Src and TESK1/p-cofilin mediated cytoskeletal F-actin pathway. Therefore, TSAIII might represent a novel strategy for the auxiliary treatment of human osteosarcoma cells.

scholarly journals Circular RNA circ_0032462 Enhances Osteosarcoma Cell Progression by Promoting KIF3B Expression

2020 ◽  
Vol 19 ◽  
pp. 153303382094321
Author(s):  
Rui Gu ◽  
Xiaodong Li ◽  
Xiaowei Yan ◽  
Zhen Feng ◽  
Aixin Hu
Keyword(s):  
Family Member ◽  
Messenger Rna ◽  
Circular Rna ◽  
Inhibitory Effect ◽  
Migration And Invasion ◽  
Circular Rnas ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Cell Progression ◽  
Kinesin Family

Circular RNAs are a recently discovered subclass of endogenous noncoding RNAs that have been confirmed to play an important role in various pathophysiological processes. However, the underlying function of circular RNAs in osteosarcoma still remains unclear. We aimed to comprehend the function of circ_0032462 in osteosarcoma, as it has been predicted to be highly expressed in osteosarcoma cells. Using real-time polymerase chain reaction, we verified the elevated expression of circ_0032462 in osteosarcoma cells than normal cells. Functional validation experiments revealed that circ_0032462 overexpression promoted proliferation, migration, and invasion in osteosarcoma cells, whereas circ_0032462 silencing was observed to inhibit cancer cell progression (proliferation, migration, and invasion). Furthermore, we found that circ_0032462 upregulated the messenger RNA and protein expression level of kinesin family member 3B. In addition, kinesin family member 3B inhibition was found to inhibit circ_0032462-induced enhanced osteosarcoma cell progression. circ_0032462 overexpression was observed to reverse circ_0032462 silencing-induced inhibitory effect on osteosarcoma cell progression. Overall, our research revealed the function of circ_0032462 in osteosarcoma progression, which might serve as a novel chemotherapeutic target for osteosarcoma.

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