Influence of the mycelium growth conditions on the production of amylolytic, proteolytic and pectinolytic enzymes by Aspergillus niger C

1989 ◽  
Vol 9 (4) ◽  
pp. 355-361 ◽  
Author(s):  
J. Fiedurek ◽  
Z. Ilczuk ◽  
J. ?obarzewski
Keyword(s):  
Aspergillus Niger ◽  
Growth Conditions ◽  
Mycelium Growth ◽  
Pectinolytic Enzymes

scholarly journals Modeling of fungal mycelium growth by fourth-class continuous stochastic cellular automaton with continuously defined growth conditions

2017 ◽  
Vol 6 (4) ◽  
pp. 98-102
Author(s):  
Anatoliy Sergeevich Shumilov ◽  
Sergey Alexandrovich Blagodatsky
Keyword(s):  
Spatial Structure ◽  
Cellular Automaton ◽  
Colony Growth ◽  
Growth Conditions ◽  
Growth Patterns ◽  
Fungal Mycelium ◽  
Mycelium Growth ◽  
Proposed Model ◽  
Area Unit ◽  
Fungal Colony

The aim of this work was to simulate the growth and spatial structure of the fungal mycelium using a cellular automaton based on the synthesis of various model approaches. The spatial structure of the mycelium is described in the structural submodel of the cellular automaton, which determines the growth rate in the direction of larger resource amount and the number of branches of the mycelium per area unit. The amount of available substrate determines the probability of unidirectional apical growth. Another, biochemical part of the model allows us to describe the rate of transport of resources into the cell, their transport within the mycelium, and also their excretion, and is intended to describe the vertical and horizontal migration in the soil of two nutrients. The proposed model makes it possible to quantitatively describe such a feature of fungal colony growth as more active absorption of resources by external cells, compared to central ones due to separation of transport resources into active and passive resources. The active transport was described using the Michaelis-Menten kinetics. We were able to simulate the stockpiling of surplus resources and their redistribution over the mycelium after the exhaustion of reserves in the external environment, and also to simulate typical growth patterns of mycelial colonies that were observed in experiments published in the literature.

Growth of morel mushroom mycelium in cheese whey

1981 ◽  
Vol 48 (1) ◽  
pp. 149-162 ◽  
Author(s):  
Naim Kosaric ◽  
Nabuo Miyata
Keyword(s):  
Unsaturated Fatty Acids ◽  
Cheese Whey ◽  
Amino Acid Content ◽  
Dry Weight ◽  
Growth Conditions ◽  
Inoculum Size ◽  
Submerged Cultivation ◽  
Mycelium Growth ◽  
Reported Data ◽  
Morel Mushroom

SummaryCheese whey was used as substrate for submerged cultivation of 8 strains of 6 species of edible mushrooms (morel mushroom):Morchella crassipes(3 strains),M. angusticeps, M. rotunda, M. deliciosa, M. esculentaand an unidentifiedMorchellasp. Best growth of morel mushroom mycelium was obtained with one of theM. crassipesstrains. The optimum growth conditions for the selected mycelium were as follows: initial pH, ~ 5·0–5·5; temperature, 25–28°C; inoculum size, 150–250 mg mycelium/100 ml whey; N sources: peptone and yeast extract; trace elements: K and Fe. More than 20 g/l mycelium was harvested in the form of pellets. Some growth kinetics studies were also performed. The initial carbohydrate (lactose) content was reduced from 5 to 0·4% at the end of the cultivation period. The specific growth rate ofM. crassipesATCC 13227 was from 1.0 to 6·4 x 10–2, depending on the growth phase. The harvested biomass contained about 45% protein, 5% fat and 8·5% ash (on a dry-weight basis). Essential amino acid content was comparable to the FAO standard, except for methionine, and unsaturated fatty acids predominated in the fat. The results with whey are compared with previously reported data on morel mushroom mycelium growth on waste sulphite liquors.

scholarly journals The AngFus3 Mitogen-Activated Protein Kinase Controls Hyphal Differentiation and Secondary Metabolism in Aspergillus niger

2015 ◽  
Vol 14 (6) ◽  
pp. 602-615 ◽  
Author(s):  
Bert-Ewald Priegnitz ◽  
Ulrike Brandt ◽  
Khomaizon A. K. Pahirulzaman ◽  
Jeroen S. Dickschat ◽  
André Fleißner
Keyword(s):  
Aspergillus Niger ◽  
Map Kinase ◽  
Secondary Metabolism ◽  
Filamentous Fungi ◽  
Cell Types ◽  
Growth Conditions ◽  
Mitogen Activated Protein Kinase ◽  
Content Type ◽  
Mitogen Activated Protein ◽  
Upstream Kinase

ABSTRACTAdaptation to a changing environment is essential for the survival and propagation of sessile organisms, such as plants or fungi. Filamentous fungi commonly respond to a worsening of their growth conditions by differentiation of asexually or sexually produced spores. The formation of these specialized cell types is, however, also triggered as part of the general life cycle by hyphal age or density. Spores typically serve for dispersal and, therefore, translocation but can also act as resting states to endure times of scarcity. Eukaryotic differentiation in response to environmental and self-derived signals is commonly mediated by three-tiered mitogen-activated protein (MAP) kinase signaling cascades. Here, we report that the MAP kinase Fus3 of the black moldAspergillus niger(AngFus3) and its upstream kinase AngSte7 control vegetative spore formation and secondary metabolism. Mutants lacking these kinases are defective in conidium induction in response to hyphal density but are fully competent in starvation-induced sporulation, indicating that conidiation inA. nigeris triggered by various independent signals. In addition, the mutants exhibit an altered profile of volatile metabolites and secrete dark pigments into the growth medium, suggesting a dysregulation of the secondary metabolism. By assigning the AngFus3 MAP kinase pathway to the transduction of a potentially self-derived trigger, this work contributes to the unraveling of the intricate signaling networks controlling fungal differentiation. Moreover, our data further support earlier observations that differentiation and secondary metabolism are tightly linked in filamentous fungi.

scholarly journals Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Sri Sugiwati ◽  
Muhammad Hanafi ◽  
Hanifah Lioe ◽  
Maggy Suhartono
Keyword(s):  
Aspergillus Niger ◽  
Rice Bran ◽  
Incubation Time ◽  
Incubation Temperature ◽  
Glycosyl Hydrolase ◽  
Fermentation Medium ◽  
Growth Conditions ◽  
Mineral Salts ◽  
Local Isolate ◽  
Substrate Ratio

Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020.  Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. β-Glucosidase is the family of glycosyl hydrolase that have potential role in various food industry, such as in tea, wine and vanilla industries to increase the aroma and production of isoflavone aglycons in soybean flour. The present work produced β-glucosidase from local isolate of Aspergillus niger InaCC F57 under solid-state fermentation (SSF) using rice bran substrate. Fermentation process was made in various conditions with respect to carbon source as substrate, initial pH of fermentation medium, incubation time, water to substrate ratio, fermentation temperature, and addition of Mandels mineral salts solution. The results showed that activity of β-glucosidase was best at, i.e., 2.45 U/mL, with the use of rice bran as substrate. Furthermore, optimum condition for the highest production of β-glucosidase occurred at pH 2.0, incubation time of 5 days, water to substrate ratio of 1.5: 1, and incubation temperature of 32°C. Additionally, in optimum fermentation conditions, production of β-glucosidase could be enhanced up to 26.22% with the presence of Mandels mineral salts solution as compared to the control.

scholarly journals The influence of the culture media composition on the white phosphorus biodegradation by Aspergillus niger

2019 ◽  
Vol 58 (5) ◽  
pp. 1-23
Author(s):  
Anton Z. Mindubaev ◽  
◽  
Elena K. Badeeva ◽  
Salima T. Minzanova ◽  
Lubov G. Mironova ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Culture Medium ◽  
Culture Media ◽  
Sole Source ◽  
Growth Conditions ◽  
White Phosphorus ◽  
The Media ◽  
Potential Basis ◽  
Living Organisms ◽  
First Time

The biodegradation of white phosphorus is undoubtedly an amazing illustration of the adaptability of living organisms to adverse environmental factors. In addition, it is a potential basis for the creation of new, breakthrough methods for detoxifying substances of the first class danger. However, establishing the fact of biological destruction is only half the battle. It is essential to optimize the growth conditions of microbial cultures and P4 biodegradation for industrial cultivation. The presented study compared the growth of Aspergillus niger strain AM1 in culture media varying in composition but containing P4 as the sole source of phosphorus. Of the ten media, two in which Aspergillus grew the fastest were selected. These media were concluded to be optimal for growth. Comparing the compositions of the media and the growth rate of Aspergillus in them, we found a key component that is a favorable factor for the growth of AM1 and the biodegradation of white phosphorus. This component was sodium nitrate (NaNO3). It has also been shown that copper sulphate (CuSO4) has no effect on the growth of Aspergillus in media with white phosphorus, regardless of the composition of these media. This result is in harmony with our previous findings. Furthermore, in the present work, attempts to increase the concentration of white phosphorus in the culture medium to values above 1% are described for the first time. For this purpose, we added the following solvents to the culture media: dimethyl sulfoxide (DMSO) and diesel, in which white phosphorus dissolves relatively well. Apparently, the presence of these substances adversely affects the growth of Aspergill. Therefore, the problem of further increasing the concentration of P4 remains an unanswered.

Effect of Media Composition and Growth Conditions on Production of β-Glucosidase by Aspergillus niger C-6

2005 ◽  
Vol 121 (1-3) ◽  
pp. 0347-0360 ◽  
Author(s):  
O. García-Kirchner ◽  
M. Segura-Granados ◽  
P. Rodríguez-Pascual
Keyword(s):  
Aspergillus Niger ◽  
Growth Conditions ◽  
Media Composition
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