Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential

2020 ◽  
Vol 4 (3) ◽  
pp. 1900254 ◽  
Author(s):  
Chih‐Ping Yu ◽  
Jyuhn‐Huarng Juang ◽  
Yu‐Jie Lin ◽  
Ching‐Wen Kuo ◽  
Li‐Hung Hsieh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Survival ◽  
Β Cell ◽  
Cell Spheroids

scholarly journals Islet Transplantation: Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential (Adv. Biosys. 3/2020)

2020 ◽  
Vol 4 (3) ◽  
pp. 2070032
Author(s):  
Chih‐Ping Yu ◽  
Jyuhn‐Huarng Juang ◽  
Yu‐Jie Lin ◽  
Ching‐Wen Kuo ◽  
Li‐Hung Hsieh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Survival ◽  
Islet Transplantation ◽  
Β Cell ◽  
Cell Spheroids

scholarly journals Vascularized Stem Cell Derived β-Cell Spheroids

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Gregory S. Korbutt ◽  
Andrew R. Pepper
Keyword(s):  
Stem Cell ◽  
Β Cell ◽  
Cell Spheroids

The Response of Testes Cells to Low Level, Single dose Helium Particle Irradiation

1982 ◽  
Vol 40 ◽  
pp. 252-253
Author(s):  
D.E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
J. Stevenson ◽  
S. Black ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Dose ◽  
B Cell ◽  
Cell Survival ◽  
Spermatogonial Stem Cell ◽  
Low Doses ◽  
Particle Irradiation ◽  
Stem Cell Survival ◽  
Irradiation Injury ◽  
Radio Sensitivity

Spermatogonial stem-cell survival after irradiation injury has been studied in rodents by histological counts of surviving cells. Many studies, including previous work from our laboratory, show that the spermatogonial population demonstrates a heterogeneous response to irradiation. The spermatogonia increase in radio-sensitivity as differentiation proceeds through the sequence As - Apr - A1 - A2 - A3 - A4 - In - B. The stem (As) cell is the most resistant and the B cell is the most sensitive. The purpose of this work is to investigate the response of spermatogonial cell to low doses (less than 10 0 rads) of helium particle irradiation.

scholarly journals Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pouria Fattahi ◽  
Ali Rahimian ◽  
Michael Q. Slama ◽  
Kihak Gwon ◽  
Alan M. Gonzalez-Suarez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Scale Up ◽  
Bioreactor Culture ◽  
Stirred Bioreactor ◽  
Cell Spheroids ◽  
Poly Ethylene ◽  
Aqueous Core ◽  
End Stage ◽  
Stirred Bioreactors

AbstractCellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.

scholarly journals Rapid Formation of Stem Cell Spheroids Using Two-Dimensional MXene Particles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 957
Author(s):  
JunHwee Jang ◽  
Eun-Jung Lee
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Processing Time ◽  
Activity Levels ◽  
Two Dimensional ◽  
Spheroid Formation ◽  
Cell Spheroids ◽  
Tissue Healing ◽  
Rapid Formation ◽  
Cell Sources

Cell spheroids have been studied as a biomimic medicine for tissue healing using cell sources. Rapid cell spheroid production increases cell survival and activity as well as the efficiency of mass production by reducing processing time. In this study, two-dimensional MXene (Ti3C2) particles were used to form mesenchymal stem cell spheroids, and the optimal MXene concentration, spheroid-production times, and bioactivity levels of spheroid cells during this process were assessed. A MXene concentration range of 1 to 10 μg/mL induced spheroid formation within 6 h. The MXene-induced spheroids exhibited osteogenic-differentiation behavior, with the highest activity levels at a concentration of 5 μg/mL. We report a novel and effective method for the rapid formation of stem cell spheroids using MXene.

Sign in / Sign up

Export Citation Format

Share Document