Label-Free Electrical Detection of DNA Hybridization on Graphene using Hall Effect Measurements: Revisiting the Sensing Mechanism

2013 ◽  
Vol 23 (18) ◽  
pp. 2301-2307 ◽  
Author(s):  
Cheng-Te Lin ◽  
Phan Thi Kim Loan ◽  
Tzu-Yin Chen ◽  
Keng-Ku Liu ◽  
Chang-Hsiao Chen ◽  
...  
Keyword(s):  
Hall Effect ◽  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection ◽  
Sensing Mechanism ◽  
Hall Effect Measurements

Study of the Sensing Mechanism of SnO2 Thin-Film Gas Sensors Using Hall Effect Measurements

2002 ◽  
Vol 230-232 ◽  
pp. 357-360 ◽  
Author(s):  
A. Lopes ◽  
Patrícia Nunes ◽  
Paula M. Vilarinho ◽  
Regina da Conceição Corredeira Monteiro ◽  
Elvira Fortunato ◽  
...  
Keyword(s):  
Thin Film ◽  
Hall Effect ◽  
Gas Sensors ◽  
Sensing Mechanism ◽  
Hall Effect Measurements

Graphene-Based Hall Effect Biosensor for Improved Specificity and Sensitivity of Label-Free DNA Detection

NANO ◽  
2020 ◽  
Vol 15 (07) ◽  
pp. 2050088
Author(s):  
Naiyuan Cui ◽  
Fei Wang ◽  
Hanyuan Ding
Keyword(s):  
Hall Effect ◽  
Dna Hybridization ◽  
Chemical Vapor ◽  
High Specificity ◽  
Label Free ◽  
Complementary Dna ◽  
Mismatched Dna ◽  
Specificity And Sensitivity ◽  
Label Free Detection ◽  
Detection Of Biomolecules

There is a broad interest in using graphene or graphene oxide (GO) sheets as a transducer for selective and label-free detection of biomolecules such as DNA, tumor marker, biological ions, etc. Here, a chemical vapor deposition (CVD) graphene-based Hall effect biosensor used for ultrasensitive label-free detection of DNA via DNA hybridization is reported. Hall effect measurements based on the Van der Pauw method are used to perform single-base sequence selective detection of DNA on graphene sheets, which are prepared by CVD. The mobility decreases and the sheet resistance increases with the adding of either complementary or one-base mismatched DNA to the graphene device. The hole carrier concentration of the graphene devices increases apparently with the addition of complementary DNA while it is hardly affected by the one-base mismatched DNA. The detection limit as low as 1[Formula: see text]pM was realized with a linear range from 1[Formula: see text]pM to 100[Formula: see text]nM. Moreover, the Hall effect biosensor was able to distinguish the complementary DNA from one-base mismatched DNA with a high specificity of [Formula: see text]6.2 which is almost two orders of magnitude higher than that of the previously reported graphene biosensors based on DNA–DNA hybridization.

Amplified label-free electrical detection of DNA hybridization

The Analyst ◽  
2002 ◽  
Vol 127 (3) ◽  
pp. 383-386 ◽  
Author(s):  
Joseph Wang ◽  
Abdel-Nasser Kawde
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection

scholarly journals Label-free electrical detection of DNA hybridization for the example of influenza virus gene sequences

2008 ◽  
Vol 374 (1) ◽  
pp. 143-153 ◽  
Author(s):  
Andreas Kukol ◽  
Peng Li ◽  
Pedro Estrela ◽  
Paul Ko-Ferrigno ◽  
Piero Migliorato
Keyword(s):  
Influenza Virus ◽  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection ◽  
Gene Sequences ◽  
Virus Gene

Semiconductor oxide based electrodes for the label-free electrical detection of DNA hybridization: Comparison between Sb doped SnO2 and CdIn2O4

2006 ◽  
Vol 51 (24) ◽  
pp. 5206-5214 ◽  
Author(s):  
V. Stambouli ◽  
A. Zebda ◽  
E. Appert ◽  
C. Guiducci ◽  
M. Labeau ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection

scholarly journals Label-free electrical detection of DNA hybridization using carbon nanotubes and graphene

Nano Reviews ◽  
2010 ◽  
Vol 1 (1) ◽  
pp. 5354 ◽  
Author(s):  
Dongliang Fu ◽  
Lain-Jong Li
Keyword(s):  
Carbon Nanotubes ◽  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection

Field-effect transistor with a chemically synthesized MoS2 sensing channel for label-free and highly sensitive electrical detection of DNA hybridization

Nano Research ◽  
2015 ◽  
Vol 8 (7) ◽  
pp. 2340-2350 ◽  
Author(s):  
Doo-Won Lee ◽  
Jinhwan Lee ◽  
Il Yung Sohn ◽  
Bo-Yeong Kim ◽  
Young Min Son ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Label Free ◽  
Electrical Detection ◽  
Highly Sensitive ◽  
Effect Transistor

Effect of Cu doping on the electrical Properties of ZnTe by Vacuum Thermal Evaporation

2018 ◽  
Vol 31 (3) ◽  
pp. 20
Author(s):  
Sarmad M. M. Ali ◽  
Alia A.A. Shehab ◽  
Samir A. Maki
Keyword(s):  
Activation Energy ◽  
Electrical Conductivity ◽  
Hall Effect ◽  
Carrier Concentration ◽  
Hall Mobility ◽  
Cu Doping ◽  
Before And After ◽  
Hall Effect Measurements ◽  
Evaporation Technique ◽  
P Type

In this study, the ZnTe thin films were deposited on a glass substrate at a thickness of 400nm using vacuum evaporation technique (2×10-5mbar) at RT. Electrical conductivity and Hall effect measurements have been investigated as a function of variation of the doping ratios (3,5,7%) of the Cu element on the thin ZnTe films. The temperature range of (25-200°C) is to record the electrical conductivity values. The results of the films have two types of transport mechanisms of free carriers with two values of activation energy (Ea1, Ea2), expect 3% Cu. The activation energy (Ea1) increased from 29meV to 157meV before and after doping (Cu at 5%) respectively. The results of Hall effect measurements of ZnTe , ZnTe:Cu films show that all films were (p-type), the carrier concentration (1.1×1020 m-3) , Hall mobility (0.464m2/V.s) for pure ZnTe film, increases the carrier concentration (6.3×1021m-3) Hall mobility (2m2/V.s) for doping (Cu at 3%) film, but  decreases by increasing Cu concentration.

Label-Free Electrochemical Detection of DNA Hybridization Related to Anthrax Lethal Factor by using Carbon Nanotube Modified Sensors

2019 ◽  
Vol 15 (4) ◽  
pp. 502-510 ◽  
Author(s):  
Hakan Karadeniz ◽  
Arzum Erdem
Keyword(s):  
Detection Limit ◽  
Dna Hybridization ◽  
Lethal Factor ◽  
Modified Electrodes ◽  
Label Free ◽  
Carbon Paste ◽  
Chip Technology ◽  
Anthrax Lethal Factor ◽  
Hybridization Time ◽  
Electrochemical Monitoring

Background: Anthrax Lethal Factor (ANT) is the dominant virulence factor produced by B. anthracis and is the major cause of death of infected animals. In this paper, pencil graphite electrodes GE were modified with single-walled and multi-walled carbon nanotubes (CNTs) for the detection of hybridization related to the ANT DNA for the first time in the literature. Methods: The electrochemical monitoring of label-free DNA hybridization related to ANT DNA was explored using both SCNT and MCNT modified PGEs with differential pulse voltammetry (DPV). The performance characteristics of ANT-DNA hybridization on disposable GEs were explored by measuring the guanine signal in terms of optimum analytical conditions; the concentration of SCNT and MCNT, the concentrations of probe and target, and also the hybridization time. Under the optimum conditions, the selectivity of probe modified electrodes was tested and the detection limit was calculated. Results: The selectivity of ANT probes immobilized onto MCNT-GEs was tested in the presence of hybridization of probe with NC no response was observed and with MM, smaller responses were observed in comparison to full-match DNA hybridization case. Even though there are unwanted substituents in the mixture samples containing both the target and NC in the ratio 1:1 and both the target and MM in the ratio 1:1, it has been found that ANT probe immobilized CNT modified graphite sensor can also select its target by resulting with 20.9% decreased response in comparison to the one measured in the case of full-match DNA hybridization case Therefore, it was concluded that the detection of direct DNA hybridization was performed by using MCNT-GEs with an acceptable selectivity. Conclusion: Disposable SCNT/MCNT modified GEs bring some important advantages to our assay including easy use, cost-effectiveness and giving a response in a shorter time compared to unmodified PGE, carbon paste electrode and glassy carbon electrode developed for electrochemical monitoring of DNA hybridization. Consequently, the detection of DNA hybridization related to the ANT DNA by MCNT modified sensors was performed by using lower CNT, probe and target concentrations, in a shorter hybridization time and resulting in a lower detection limit according to the SCNT modified sensors. In conclusion, MCNT modified sensors can yield the possibilities leading to the development of nucleic acid sensors platforms for the improvement of fast and cost-effective detection systems with respect to DNA chip technology.

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