scholarly journals 3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host‐To‐Implant Perfusion (Adv. Mater. 42/2021)

2021 ◽  
Vol 33 (42) ◽  
pp. 2170335
Author(s):  
Ariel A. Szklanny ◽  
Majd Machour ◽  
Idan Redenski ◽  
Václav Chochola ◽  
Idit Goldfracht ◽  
...  
Keyword(s):  
3D Bioprinting ◽  
Engineered Tissue ◽  
Vessel Networks ◽  
Tissue Flaps

scholarly journals 3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host‐To‐Implant Perfusion

2021 ◽  
pp. 2102661
Author(s):  
Ariel A. Szklanny ◽  
Majd Machour ◽  
Idan Redenski ◽  
Václav Chochola ◽  
Idit Goldfracht ◽  
...  
Keyword(s):  
3D Bioprinting ◽  
Engineered Tissue ◽  
Vessel Networks ◽  
Tissue Flaps

scholarly journals 3D bioprinting of anisotropic engineered tissue constructs with ultrasonically induced cell patterning

2020 ◽  
Vol 32 ◽  
pp. 101042 ◽  
Author(s):  
Parth Chansoria ◽  
Rohan Shirwaiker
Keyword(s):  
Cell Patterning ◽  
3D Bioprinting ◽  
Tissue Constructs ◽  
Engineered Tissue

Effects of Collagen Microstructure on the Transport Properties of Vascularized Engineered Tissues

2012 ◽  
Author(s):  
Seungman Park ◽  
Catherine Whittington ◽  
Mervin C. Yoder ◽  
Sherry Voytik-Harbin ◽  
Bumsoo Han
Keyword(s):  
The Body ◽  
Vascular Perfusion ◽  
Impaired Wound Healing ◽  
Disease States ◽  
Progression Of Disease ◽  
Transplant Failure ◽  
Organ Tissue ◽  
Engineered Tissue ◽  
Vessel Networks ◽  
And Function

When vascular perfusion is compromised within the body, a number of physiological phenomena can occur, including the progression of disease states (e.g. peripheral vascular disease), impaired wound healing, and organ/tissue transplant failure. Therefore, there is a need for an engineered tissue construct that promotes therapeutic vasculogenesis by enhancing the formation of functional, stabilized vessel networks that have the ability to integrate with the host vasculature. In order to restore tissue structure and function, a matrix-based delivery system, which combines a biopolymer and endothelial precursor cells, is necessary to ensure the localization and guidance of vessel formation and stabilization.

scholarly journals Mature vessel networks in engineered tissue promote graft–host anastomosis and prevent graft thrombosis

2019 ◽  
Vol 116 (8) ◽  
pp. 2955-2960 ◽  
Author(s):  
Shahar Ben-Shaul ◽  
Shira Landau ◽  
Uri Merdler ◽  
Shulamit Levenberg
Keyword(s):  
Thrombus Formation ◽  
Coagulation Factors ◽  
Graft Thrombosis ◽  
Host Interactions ◽  
Engineered Tissues ◽  
Elevated Expression ◽  
Vessel Maturation ◽  
Engineered Tissue ◽  
Vessel Networks

Graft vascularization remains one of the most critical challenges facing tissue-engineering experts in their attempt to create thick transplantable tissues and organs. In vitro prevascularization of engineered tissues has been suggested to promote rapid anastomosis between the graft and host vasculatures; however, thrombotic events have been reported upon graft implantation. Here, we aimed to determine whether in vitro vessel maturation in transplantable grafts can accelerate vascular integration and graft perfusion and prevent thrombotic events in the grafts. To this end, endothelial cells and fibroblasts were cocultured on 3D scaffolds for 1, 7, or 14 d to form vasculature with different maturation degrees. Monitoring graft–host interactions postimplantation demonstrated that the 14-d in vitro-cultured grafts, bearing more mature and complex vessel networks as indicated by elongated and branched vessel structures, had increased graft–host vessel anastomosis; host vessel penetration into the graft increased approximately eightfold, and graft perfusion increased sixfold. The presence of developed vessel networks prevented clot accumulation in the grafts. Conversely, short-term cultured constructs demonstrated poor vascularization and increased thrombus formation. Elevated expression levels of coagulation factors, von Willebrand factor (vWF), and tissue factor (TF), were demonstrated in constructs bearing less mature vasculature. To conclude, these findings demonstrate the importance of establishing mature and complex vessel networks in engineered tissues before implantation to promote anastomosis with the host and accelerate graft perfusion.

scholarly journals Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

2021 ◽  
Author(s):  
Mohsen Askari ◽  
Moqaddaseh Afzali Naniz ◽  
Monireh Kouhi ◽  
Azadeh Saberi ◽  
Ali Zolfagharian ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Recent Progress ◽  
3D Bioprinting ◽  
Comprehensive Review ◽  
Tissue Constructs ◽  
Research Communities ◽  
Engineered Tissue

Over the last decade, 3D bioprinting has received immense attention from research communities to bridge the divergence between artificially engineered tissue constructs and native tissues.

Evaluation of conduction through engineered tissue in implanted hearts by high resolution optical surface mapping

2004 ◽  
Vol 52 (S 1) ◽  
Author(s):  
YH Choi ◽  
PE Hammer ◽  
C Stamm ◽  
I Friehs ◽  
KF Kwaku ◽  
...  
Keyword(s):  
High Resolution ◽  
Optical Surface ◽  
Surface Mapping ◽  
Engineered Tissue

scholarly journals Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 971
Author(s):  
Young Soo Yu ◽  
Chi Bum Ahn ◽  
Kuk Hui Son ◽  
Jin Woo Lee
Keyword(s):  
Rotation Angle ◽  
Thick Layer ◽  
Cartilage Tissue ◽  
Soft Tissue Reconstruction ◽  
3D Bioprinting ◽  
Rotating Beam ◽  
Rotational Angle ◽  
Gelatin Hydrogel ◽  
Tissue Reconstruction ◽  
Neck Rotation

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.

scholarly journals A 3D Bioprinted Material That Recapitulates the Perivascular Bone Marrow Structure for Sustained Hematopoietic and Cancer Models

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 480
Author(s):  
Caitlyn A. Moore ◽  
Zain Siddiqui ◽  
Griffin J. Carney ◽  
Yahaira Naaldijk ◽  
Khadidiatou Guiro ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Translational Medicine ◽  
Drug Response ◽  
Drug Approval ◽  
3D Models ◽  
3D Bioprinting ◽  
Effective Evaluation ◽  
Experimental Systems ◽  
Cancer Models

Translational medicine requires facile experimental systems to replicate the dynamic biological systems of diseases. Drug approval continues to lag, partly due to incongruencies in the research pipeline that traditionally involve 2D models, which could be improved with 3D models. The bone marrow (BM) poses challenges to harvest as an intact organ, making it difficult to study disease processes such as breast cancer (BC) survival in BM, and to effective evaluation of drug response in BM. Furthermore, it is a challenge to develop 3D BM structures due to its weak physical properties, and complex hierarchical structure and cellular landscape. To address this, we leveraged 3D bioprinting to create a BM structure with varied methylcellulose (M): alginate (A) ratios. We selected hydrogels containing 4% (w/v) M and 2% (w/v) A, which recapitulates rheological and ultrastructural features of the BM while maintaining stability in culture. This hydrogel sustained the culture of two key primary BM microenvironmental cells found at the perivascular region, mesenchymal stem cells and endothelial cells. More importantly, the scaffold showed evidence of cell autonomous dedifferentiation of BC cells to cancer stem cell properties. This scaffold could be the platform to create BM models for various diseases and also for drug screening.

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