scholarly journals A nuclear phylogenomic study of the angiosperm order Myrtales, exploring the potential and limitations of the universal Angiosperms353 probe set

2021 ◽  
Author(s):  
Olivier Maurin ◽  
Artemis Anest ◽  
Sidonie Bellot ◽  
Edward Biffin ◽  
Grace Brewer ◽  
...  
Keyword(s):  
Probe Set ◽  
Phylogenomic Study

Hemorrhoidal Laser procedure (HeLP) and Hemorrrrhoidal Laser procedure + mucopexy (HeLPexx) and other Emerging Technologies

2020 ◽  
Vol 15 ◽  
Author(s):  
Paolo Giamundo
Keyword(s):  
Laser Treatment ◽  
Dentate Line ◽  
Mucosal Prolapse ◽  
Daily Activities ◽  
Common Goal ◽  
Superior Rectal Artery ◽  
Doppler Probe ◽  
The Common ◽  
Probe Set

Background: Minimally-invasive treatments for hemorrhoids should be encouraged as they cause low morbidity, reasonable discomfort and quicker return to work. According to the “vascular theory” hemorrhoidal disease is mainly caused by blood overflow into hemorrhoidal plexus deriving from the superior hemorrhoidal arteries. Introduction: Many different procedures have been described in the literature with the common goal of reducing the blood flow into the hemorrhoidal piles. ‘HeLP’ (Hemorrhoids Laser Procedure) is a novel form of dearterialization to treat patients suffering from symptomatic hemorrhoids. Methods: The procedure consists of the closure of the terminal branches of the superior rectal artery approximately 2-3 cm above the dentate line by means of laser shots originated by a diode laser platform. The arteries, at that level, have variable location and distribution. Therefore, a doppler probe set at the frequency of 20MHz helps identifying the arteries that would be missed otherwise. The laser beam is well tolerated by patients. For this reason, anesthesia is not required in most cases and the procedure allows a quick return to daily activities. In case of concomitant severe mucosal prolapse, the laser treatment can be combined with suture mucopexy. Three to six running sutures allow a complete lifting of hemorrhoidal piles, securing long-term resolution of symptoms. Results and Conclusions: ‘HeLP’ is indicated in patients with symptomatic hemorrhoids where conservative treatment failed and when mucosal prolapse is scarce or not symptomatic. The addition of mucopexy to laser treatment (HeLPexx) contributes to overall resolution of symptoms when mucosal prolapse is an issue, Emborrhoid is another novel, ‘hi-tech’ form of selective dearterialization used in selected case of hemorrhoids where main symptom is bleeding. It is generally used in cases where surgery is contraindicated due to severe concomitant diseases.

scholarly journals A target enrichment probe set for resolving the flagellate land plant tree of life

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Jesse W. Breinholt ◽  
Sarah B. Carey ◽  
George P. Tiley ◽  
E. Christine Davis ◽  
Lorena Endara ◽  
...  
Keyword(s):  
Land Plant ◽  
Tree Of Life ◽  
Target Enrichment ◽  
Probe Set

Weather Probe Set near Barbados

Science News ◽  
1969 ◽  
Vol 95 (17) ◽  
pp. 411 ◽  
Author(s):  
Ann Ewing
Keyword(s):  
Probe Set

Phylogenomic study of the subfamily Caprinae by cross-species chromosome painting with Chinese muntjac paints

2005 ◽  
Vol 13 (4) ◽  
pp. 389-399 ◽  
Author(s):  
Ling Huang ◽  
Wenhui Nie ◽  
Jinhuan Wang ◽  
Weiting Su ◽  
Fengtang Yang
Keyword(s):  
Chromosome Painting ◽  
Cross Species Chromosome Painting ◽  
Phylogenomic Study

Corrosion surveillance program for tank, fuel cladding and supporting structure of 30 MW Indonesian RSG GAS research reactor

Kerntechnik ◽  
2021 ◽  
Vol 86 (3) ◽  
pp. 236-243
Author(s):  
G. R. Sunaryo ◽  
R. Kusumastuti ◽  
Sriyono
Keyword(s):  
Water Quality ◽  
Power Plants ◽  
Research Reactor ◽  
Visual Analysis ◽  
Galvanic Corrosion ◽  
Steel Wire ◽  
Chloride Ions ◽  
Bottom Surface ◽  
Surveillance Program ◽  
Probe Set

Abstract The objective of this research is to understand the condition of the structural material of the 30 MW RSG-GAS research reactor as input for the aging management program. Furthermore, this should enable a prediction of the remaining life of the components. In the current experiment, corrosion surveillance was carried out at Interim Storage for Spent Fuel (ISSF), that has similar water quality as in reactor pool by using a corrosion probe which is made of aluminum alloy and stainless steel. The probe set is designed to understand the effect of water quality in the ISSF pond. The corrosion processes observed were pitting, crevice and galvanic corrosion. Two sets of corrosion probes were immersed into the ISSF pool in 2007, hanging by steel wire, 1-meter height from the bottom surface. One probe set consists of horizontal and vertical positions. The soaking time was 7 years. The observations made were water chemical content, corrosion rate and visual analysis, macro and micro. For macro visual observations an optical microscope was used, for micro-observations SEM-EDX. From the results of macro-observations, information on the presence of galvanic corrosion, crevice and pitting was obtained. SEM-EDX provides information on the influence of chloride ions on corrosion products. This experience will be very useful in dealing with the aging process of Indonesia’s nuclear power plants in the future.

A novel exome probe set captures phototransduction genes across birds (Aves) enabling efficient analysis of vision evolution

2021 ◽  
Author(s):  
Noor D. White ◽  
Zachary A. Batz ◽  
Edward L. Braun ◽  
Michael J. Braun ◽  
Karen L. Carleton ◽  
...  
Keyword(s):  
Probe Set

scholarly journals A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering

2018 ◽  
Vol 68 (4) ◽  
pp. 594-606 ◽  
Author(s):  
Matthew G Johnson ◽  
Lisa Pokorny ◽  
Steven Dodsworth ◽  
Laura R Botigué ◽  
Robyn S Cowan ◽  
...  
Keyword(s):  
Nuclear Genes ◽  
Targeted Sequencing ◽  
Flowering Plant ◽  
Probe Set

scholarly journals Tetraconatan phylogeny with special focus on Malacostraca and Branchiopoda: highlighting the strength of taxon-specific matrices in phylogenomics

2018 ◽  
Vol 285 (1885) ◽  
pp. 20181524 ◽  
Author(s):  
Martin Schwentner ◽  
Stefan Richter ◽  
D. Christopher Rogers ◽  
Gonzalo Giribet
Keyword(s):  
Amino Acid ◽  
Analytical Methods ◽  
Special Focus ◽  
Evolutionary Models ◽  
Phylogenomic Analyses ◽  
New Generation ◽  
Analytical Approaches ◽  
Internal Relationships ◽  
Complete Matrix ◽  
Phylogenomic Study

Understanding the evolution of Tetraconata or Pancrustacea—the clade that includes crustaceans and insects—requires a well-resolved hypothesis regarding the relationships within and among its constituent taxa. Here, we assembled a taxon-rich phylogenomic dataset focusing on crustacean lineages based solely on genomes and new-generation Illumina-generated transcriptomes, including 89 representatives of Tetraconata. This constitutes, to our knowledge, the first phylogenomic study specifically addressing internal relationships of Malacostraca (with 26 species included) and Branchiopoda (36 species). Seven matrices comprising 81–684 orthogroups and 17 690–242 530 amino acid positions were assembled and analysed under five different analytical approaches. To maximize gene occupancy and to improve resolution, taxon-specific matrices were designed for Malacostraca and Branchiopoda. Key tetraconatan taxa (i.e. Oligostraca, Multicrustacea, Branchiopoda, Malacostraca, Thecostraca, Copepoda and Hexapoda) were monophyletic and well supported. Within Branchiopoda, Phyllopoda, Diplostraca, Cladoceromorpha and Cladocera were monophyletic. Within Malacostraca, the clades Eumalacostraca, Decapoda and Reptantia were well supported. Recovery of Caridoida or Peracarida was highly dependent on the analysis for the complete matrix, but it was consistently monophyletic in the malacostracan-specific matrices. From such examples, we demonstrate that taxon-specific matrices and particular evolutionary models and analytical methods, namely CAT-GTR and Dayhoff recoding, outperform other approaches in resolving certain recalcitrant nodes in phylogenomic analyses.

scholarly journals Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals

2020 ◽  
Author(s):  
Harsha Doddapaneni ◽  
Sara Javornik Cregeen ◽  
Richard Sucgang ◽  
Qingchang Meng ◽  
Xiang Qin ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Oligonucleotide Probe ◽  
Threshold Value ◽  
Open Reading Frames ◽  
Clinical Samples ◽  
Viral Loads ◽  
Full Length Genome ◽  
Probe Set ◽  
Reading Frames ◽  
Capture Sequencing

AbstractThe newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open reading frames [ORFs]) sequences from 45 SARS-CoV-2 clinical samples with varying viral titers. For samples with higher viral loads (cycle threshold value under 33, based on the CDC qPCR assay) complete genomes were generated. Analysis of junction reads revealed regions of differential transcriptional activity and provided evidence of expression of ORF10. Heterogeneous allelic frequencies along the 20kb ORF1ab gene suggested the presence of a defective interfering viral RNA species subpopulation in one sample. The associated workflow is straightforward, and hybridization-based capture offers an effective and scalable approach for sequencing SARS-CoV-2 from patient samples.

scholarly journals An optimized ribodepletion approach for C. elegans RNA-sequencing libraries

2021 ◽  
Author(s):  
Alec Barrett ◽  
Rebecca McWhirter ◽  
Seth R Taylor ◽  
Alexis Weinreb ◽  
David M Miller ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Noncoding Rnas ◽  
Rrna Gene ◽  
Library Preparation ◽  
C Elegans ◽  
Pcr Duplicates ◽  
Rrna Depletion ◽  
Dissociated Cells ◽  
Probe Set ◽  
Long Genes

ABSTRACTA recent and powerful technique is to obtain transcriptomes from rare cell populations, such as single neurons in C. elegans, by enriching dissociated cells using fluorescent sorting. However, these cell samples often have low yields of RNA that present challenges in library preparation. This can lead to PCR duplicates, noisy gene expression for lowly expressed genes, and other issues that limit endpoint analysis. Further, some common resources, such as sequence specific kits for removing ribosomal RNA, are not optimized for non-mammalian samples. To optimize library construction for such challenging samples, we compared two approaches for building RNAseq libraries from less than 10 nanograms of C. elegans RNA: SMARTSeq V4 (Takara), a widely used kit for selecting poly-adenylated transcripts; and SoLo Ovation (Tecan Genomics), a newly developed ribodepletion-based approach. For ribodepletion, we used a custom kit of 200 probes designed to match C. elegans rRNA gene sequences. We found that SoLo Ovation, in combination with our custom C. elegans probe set for rRNA depletion, detects an expanded set of noncoding RNAs, shows reduced noise in lowly expressed genes, and more accurately counts expression of long genes. The approach described here should be broadly useful for similar efforts to analyze transcriptomics when RNA is limiting.

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