Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

2006 ◽  
Vol 100 (1) ◽  
pp. 495-499 ◽  
Author(s):  
Yuichi Tada ◽  
Kimio Sumaru ◽  
Mitsuyoshi Kameda ◽  
Katsuhide Ohi ◽  
Toshiyuki Takagi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Living Cell ◽  
Light Irradiation ◽  
Culture Surface

scholarly journals Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 171-177
Author(s):  
Nobuyuki Tanaka ◽  
Ryoji Sekine ◽  
Shun-ichi Funano ◽  
Asako Sato ◽  
Núria Taberner Carretero ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Polymer Film ◽  
Cell Patterning ◽  
Ethanol Solution ◽  
Rapid Fabrication ◽  
A Cell ◽  
Culture Surface

This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.

scholarly journals On-Demand Control of Living Cell Adhesion by Local Light Irradiation

2007 ◽  
Vol 35 (7) ◽  
pp. 436-440
Author(s):  
Kimio SUMARU ◽  
Toshiyuki KANAMORI
Keyword(s):  
Cell Adhesion ◽  
Living Cell ◽  
Light Irradiation ◽  
On Demand ◽  
Demand Control

Changing ligand number and type within nanocylindrical domains through kinetically constrained self-assembly – impacts of ligand ‘redundancy’ on human mesenchymal stem cell adhesion and morphology

2014 ◽  
Vol 2 (11) ◽  
pp. 1693-1705 ◽  
Author(s):  
Haiqing Li ◽  
Justin J. Cooper-White
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Block Copolymer ◽  
Mesenchymal Stem Cell ◽  
Click Chemistry ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cell ◽  
Culture Surface ◽  
Self Assembled

In this paper, we firstly describe a facile method by which sequential attachment of different adhesion peptides to a nanotopographical, self-assembled block copolymer cell culture surface is made possible through orthogonal click chemistry.

scholarly journals Focused surface acoustic wave locally removes cells from culture surface

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Takumi Inui ◽  
Jiyang Mei ◽  
Chikahiro Imashiro ◽  
Yuta Kurashina ◽  
James Friend ◽  
...  
Keyword(s):  
Cell Culture ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Water Droplet ◽  
C2c12 Cells ◽  
Area 6 ◽  
Culture Surface

After exposing a plated C2C12 cells culture to PBS, ultrasound from the SAW device transmitted into the cell culture via a coupling water droplet. We can remove cells from an area 6 × 10−3 mm2, equivalent to about 12 cells.

scholarly journals Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3042
Author(s):  
Eun Ju Lee ◽  
Khurshid Ahmad ◽  
Shiva Pathak ◽  
SunJu Lee ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Adhesion ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Primary Cells ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Promote Cell Adhesion

In recent years, a major rise in the demand for biotherapeutic drugs has centered on enhancing the quality and efficacy of cell culture and developing new cell culture techniques. Here, we report fibronectin (FN) derived, novel peptides fibronectin-based intergrin binding peptide (FNIN)2 (18-mer) and FNIN3 (20-mer) which promote cell adhesion proliferation, and the differentiation of primary cells and stem cells. FNIN2 and 3 were designed based on the in silico interaction studies between FN and its receptors (integrin α5β1, αvβ3, and αIIbβ3). Analysis of the proliferation of seventeen-cell types showed that the effects of FNINs depend on their concentration and the existence of expressed integrins. Significant rhodamine-labeled FNIN2 fluorescence on the membranes of HeLa, HepG2, A498, and Du145 cells confirmed physical binding. Double coating with FNIN2 or 3 after polymerized dopamine (pDa) or polymerized tannic acid (pTA) precoating increased HBEpIC cell proliferation by 30–40 percent, suggesting FNINs potently affect primary cells. Furthermore, the proliferation of C2C12 myoblasts and human mesenchymal stem cells (MSCs) treated with FNINs was significantly increased in 2D/3D culture. FNINs also promoted MSC differentiation into osteoblasts. The results of this study offer a new approach to the production of core materials (e.g., cell culture medium components, scaffolds) for cell culture.

scholarly journals Development of a Dynamic Stem Cell Culture Platform for Mesenchymal Stem Cell Adhesion and Evaluation

2014 ◽  
Vol 11 (7) ◽  
pp. 2172-2181 ◽  
Author(s):  
Kimberly M. Ferlin ◽  
Margaret E. Prendergast ◽  
Makenzie L. Miller ◽  
Bao-Ngoc B. Nguyen ◽  
David S. Kaplan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Culture

Cell Adhesion in Animal Cell Culture: Physiological and Fluid-Mechanical Implications

Cell Adhesion ◽  
2018 ◽  
pp. 61-110
Author(s):  
Manfred R. Koller ◽  
Eleftherios T. Papoutsakis
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Animal Cell ◽  
Animal Cell Culture
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