scholarly journals Thick Methacrylate Sections Devoid of Lost Caps Simplify Stereological Quantifications Based on the Optical Fractionator Design

2015 ◽  
Vol 298 (12) ◽  
pp. 2141-2150 ◽  
Author(s):  
Stine Hasselholt ◽  
Jens Lykkesfeldt ◽  
Jytte Overgaard Larsen
Keyword(s):  
Optical Fractionator

Environmental enrichment increases the number of telencephalic but not tectal cells of angelfish (Pterophyllum scalare): an exploratory investigation using optical fractionator

2020 ◽  
Vol 103 (7) ◽  
pp. 847-857
Author(s):  
Daniel Guerreiro Diniz ◽  
Lucas Silva de Siqueira ◽  
Ediely Pereira Henrique ◽  
Patrick Douglas Correa Pereira ◽  
Cristovam Guerreiro Diniz ◽  
...  
Keyword(s):  
Environmental Enrichment ◽  
Exploratory Investigation ◽  
Optical Fractionator ◽  
Pterophyllum Scalare

Use of frozen sections to determine neuronal number in the murine hippocampus and neocortex using the optical disector and optical fractionator

2004 ◽  
Vol 14 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Daniel J. Bonthius ◽  
Ross McKim ◽  
Lindsey Koele ◽  
Harb Harb ◽  
Bahri Karacay ◽  
...  
Keyword(s):  
Frozen Sections ◽  
Optical Disector ◽  
Neuronal Number ◽  
Optical Fractionator

scholarly journals Estimation of the C-cell numbers in rat thyroid glands using the optical fractionator.

1996 ◽  
Vol 44 (9) ◽  
pp. 997-1003 ◽  
Author(s):  
R E Feinstein ◽  
E Westergren ◽  
E Bucht ◽  
H E Sjöberg ◽  
L Grimelius
Keyword(s):  
Serum Levels ◽  
C Cells ◽  
Stereological Method ◽  
Cell Numbers ◽  
C Cell ◽  
Glass Slides ◽  
Thyroid Glands ◽  
Optical Fractionator ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

We estimated the total number of calcitonin-immunoreactive C-cells in rat thyroid gland using the optical fractionator, the unbiased stereological method for estimation of numbers. It was necessary first to use a fixative composed of formalin, acetic acid, and ethanol to distinctly visualize the C-cells. The 40-microm-thick sections had to adhere to chromalum-gelatin-coated Superfrost Plus glass slides, and the immunostaining technique had to stain the C-cells evenly throughout the whole sections. Because the C-cells were irregularly distributed in the thyroid tissues, their counting required screening of about 500 fields per lobe, but the number of C-cells counted need not be high, about 90 per lobe. We estimated that rats have 185,000 +/- 42,000 C-cells (mean +/- SD; n - 7). The C-cell population did not differ significantly between the two lobes of a given rat, but it varied markedly among rats. The biological differences among the animals contributed 83% to the observed variability, whereas the methodological uncertainty contributed 17%. The serum levels of calcitonin and calcium were not closely correlated to the C-cell numbers. Our results indicate that variability in C-cell experiments can be reduced most effectively by increasing the number of animals used. However, the similar C-cell frequency found in the two thyroid lobes of each rat allows the use of one uniformly sampled lobe for quantification and the other lobe for further analysis.

A stereological analysis of the lateral geniculate nucleus in adult Macaca nemestrina monkeys

1999 ◽  
Vol 16 (5) ◽  
pp. 933-941 ◽  
Author(s):  
BEATRIZ BLASCO ◽  
CARLOS AVENDAÑO ◽  
CARMEN CAVADA
Keyword(s):  
Lateral Geniculate Nucleus ◽  
Macaca Nemestrina ◽  
Dorsal Lateral Geniculate Nucleus ◽  
Published Data ◽  
Quantitative Studies ◽  
Lateral Geniculate ◽  
Unbiased Estimates ◽  
Optical Fractionator ◽  
Dorsal Lateral Geniculate ◽  
Volume Estimates

The Cavalieri method and the optical fractionator were employed to estimate the volume and neuron numbers, respectively, of the dorsal lateral geniculate nucleus (dLGN) in seven adult male pigtail monkeys (Macaca nemestrina). Unbiased estimates were selectively obtained for the parvocellular (P), magnocellular (M), and interlaminar plus superficial (I + S) layers of the nucleus. The dLGN had a mean volume of 56.5 mm3, and contained on average 1.79 million neurons. The P layers contributed 64% of the volume and 83% of the neurons in the dLGN; the corresponding proportions for the other layers were 13% and 9% (M), and 23% and 8% (I + S). Interindividual variability was large for neuron counts, which varied within a two-fold range, and lower for volume estimates. Since no published data are available for the pigtail dLGN, the present results are compared with quantitative studies of the dLGN in other macaque species, placing special emphasis in the discussion of the methodologies used.

scholarly journals Unbiased estimation of cell number using the automatic optical fractionator

2017 ◽  
Vol 80 ◽  
pp. A1-A8 ◽  
Author(s):  
Peter R. Mouton ◽  
Hady Ahmady Phoulady ◽  
Dmitry Goldgof ◽  
Lawrence O. Hall ◽  
Marcia Gordon ◽  
...  
Keyword(s):  
Cell Number ◽  
Unbiased Estimation ◽  
Optical Fractionator

scholarly journals Effect of atorvastatin on spermatogenesis in rats: A stereological study

2021 ◽  
Vol 19 (12) ◽  
pp. 2609-2614
Author(s):  
Ekrem Akdeniz ◽  
Mehmet Emin Onger ◽  
Mustafa Suat Bolat ◽  
Fatih Firat ◽  
Metin Gur ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Study Groups ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Total Testosterone ◽  
Cholesterol Levels ◽  
Significant Difference ◽  
Optical Fractionator ◽  
Rat Spermatogenesis

Purpose: To investigate the effects of oral atorvastatin on spermatogenesis in a rat model.Methods: Rats were equally assigned into control and study groups, the latter receiving atorvastatin (20 mg/kg/day). At the end of 12 weeks, spermatogenetic activity was evaluated using stereological and optical fractionator methods. Serum follicle-stimulating hormone (FSH), total testosterone (TT), and luteinizing hormone (LH) levels were measured using micro–ELISA kits. Total cholesterol, triglyceride (TG), low-density lipoprotein cholesterol (LDL - C), and high-density lipoprotein cholesterol levels were also measured by enzymatic colorimetric assays.Results: Testicular stereological analysis revealed that atorvastatin reduced Sertoli cell numbers (p < 0.001), spermatogonia (p < 0.001), spermatocytes (p < 0.001), and seminiferous tubule diameters (p < 0.001). LDL – C (p = 0.01) and TG (p = 0.01) values were significantly lower in the study group compared with the control group. There was no significant difference in FSH (p = 0.44), LH (p = 0.48),and TT (p = 0.06) levels between the groups.Conclusion: The findings show that atorvastatin causes deleterious effects on rat spermatogenesis. It should therefore be used with caution in clinical practice owing to its potential adverse effects, especially on male fertility. Keywords: Statin, Atorvastatin, Spermatogenesis, Stereology, Testis

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