scholarly journals Maximizing Explanatory Power in Stereological Data Collection: A Protocol for Reliably Integrating Optical Fractionator and Multiple Immunofluorescence Techniques

2018 ◽  
Vol 12 ◽  
Author(s):  
Anna Kreutz ◽  
Nicole Barger
Author(s):  
Jimmy Lin

Over the past few years, we have seen the emergence of “big data”: disruptive technologies that have transformed commerce, science, and many aspects of society. Despite the tremendous enthusiasm for big data, there is no shortage of detractors. This article argues that many criticisms stem from a fundamental confusion over goals: whether the desired outcome of big data use is “better science” or “better engineering.” Critics point to the rejection of traditional data collection and analysis methods, confusion between correlation and causation, and an indifference to models with explanatory power. From the perspective of advancing social science, these are valid reservations. I contend, however, that if the end goal of big data use is to engineer computational artifacts that are more effective according to well-defined metrics, then whatever improves those metrics should be exploited without prejudice. Sound scientific reasoning, while helpful, is not necessary to improve engineering. Understanding the distinction between science and engineering resolves many of the apparent controversies surrounding big data and helps to clarify the criteria by which contributions should be assessed.


Author(s):  
Betina Hollstein

This commentary focuses on promises and pitfalls of qualitative longitudinal research (QLR). Longitudinal data on practices, perspectives, individual relevancies and experiences can be particularly advantageous for life course, social policy and health research. However, the complexity of QLR carries certain downsides, dilemmas and trade-offs. The commentary discusses implications of different qualitative methods for the investigation of stability and change, the tensions between flexibility and comparability, and challenges related to sampling and the explanatory power of QLR.It is argued that the choices of methods for data collection and data analysis have stark implications for what can be determined as change and stability across time. In addition, several ways of ensuring comparability across time are described. Finally, sampling strategies are outlined that aim to achieve and maintain heterogeneity of cases.As standards of good practice, the author advocates more thorough documentation of the methods of data collection, sampling and data analysis employed in QLR studies, along with a better description of instruments and how they are applied.<br />Key messages<br /><ul><li>In QLR the choice of methods have implications for what can be determined as change across time.</li><br /><li>Several ways of ensuring comparability across time are described.</li><br /><li>Sampling strategies are outlined aiming to achieve heterogeneity of cases.</li></ul>


2007 ◽  
Vol 10 (4) ◽  
pp. 41-51
Author(s):  
John Goldthorpe

The present crisis in sociology: a way beyond spurious pluralism? At the end of the 20th century the state of sociology gives cause for serious con¬cern. The main reasons for this concern can be stated in three features of contem¬porary sociology: First, there is a manifest lack of integration of research and theory. This is a long-standing difficulty, but what makes it worse today than pre¬viously is, that it is now less often seen as a serious problem. Second, there is an evident collective failure among sociolo¬gists to decide just what kind of discip¬line sociology is or ought to be. For some sociology should aim to be a social scien¬ce and to have therefore well-defined links with other social sciences, such as economics and political science, and al¬so human sciences. For others such aspi¬rations represent an outmoded ”posi¬tivsm”. If sociology is to be thought of as a social science at all then it must be one of a distinctive kind; and the crucial inter¬disciplinary links should be with cer¬tains kinds of philosophy and with cul¬tural studies. Third and finally, there is disagreement about how the disagree¬ments on the nature of sociology should itself be viewed. Despite the state of intellectual disar¬ray today´s sociology has some signifi¬cant achievements which can be charac¬terised as success stories and two are mentioned in the article. The first suc¬cess story is about the quantitative soci¬al research: more specifically, research that involves both data collection and da¬ta analysis that are based on statistical methods and on the theory of probability. Through statistical modelling know¬ledge about important social regularities has been established and these social re¬gularities are of major theoretical signi¬ficance. The modelling has typically ena¬bled sociologists to separate out more clearly than before what are the probabi¬litic regularities inherent in complex da¬ta-sets. The second success story of sociology is that of the theory of social action. Today, if we want to have an effective kind of sociological theory then it will be a theory of social action of some kind or other. Two developments lead to this conclu¬sion. The first is the evident collapse of functionalist theory over the last two to three decades. For functionalism to have explanatory power it is necessary that the systems which are taken as the units of analysis should exists in a selective environment; i. e. there must be the possi¬bility that the systems will in some sense fail to survive. Then it becomes possible to explain their constituent feature by re¬ference to their ”survival value”. This ap¬proach underlines the need for sociologi¬cal explannation to have a ”micro-foun¬dation”: i.e. to comprise not only ”macro¬-to-micro” link but a ”micro-to-macro” link as well. We need to return to the in¬dividualistic tradition in sociology. The second argument goes as following: Wi¬thin the individualistic tradition, theory based on the concept of action has in fact shown much greater promise than the main alternative has: i. e. theory based on the concept of behaviour. The attempts to revitalise the individualistic tradition via the theory of social action has proved rewarding and it seems to be around rational action theory that we may best try to build up a more general theory of social action. Through the statistical modelling ba¬sed on data collection and the theory of probability on the one hand and through developing a theory of rational social ac¬tion within the individualistic tradition on the other hand we might be able to overcome the present difficulties of socio¬logy as a science.


Author(s):  
S.W. Hui ◽  
D.F. Parsons

The development of the hydration stages for electron microscopes has opened up the application of electron diffraction in the study of biological membranes. Membrane specimen can now be observed without the artifacts introduced during drying, fixation and staining. The advantages of the electron diffraction technique, such as the abilities to observe small areas and thin specimens, to image and to screen impurities, to vary the camera length, and to reduce data collection time are fully utilized. Here we report our pioneering work in this area.


Author(s):  
Weiping Liu ◽  
Jennifer Fung ◽  
W.J. de Ruijter ◽  
Hans Chen ◽  
John W. Sedat ◽  
...  

Electron tomography is a technique where many projections of an object are collected from the transmission electron microscope (TEM), and are then used to reconstruct the object in its entirety, allowing internal structure to be viewed. As vital as is the 3-D structural information and with no other 3-D imaging technique to compete in its resolution range, electron tomography of amorphous structures has been exercised only sporadically over the last ten years. Its general lack of popularity can be attributed to the tediousness of the entire process starting from the data collection, image processing for reconstruction, and extending to the 3-D image analysis. We have been investing effort to automate all aspects of electron tomography. Our systems of data collection and tomographic image processing will be briefly described.To date, we have developed a second generation automated data collection system based on an SGI workstation (Fig. 1) (The previous version used a micro VAX). The computer takes full control of the microscope operations with its graphical menu driven environment. This is made possible by the direct digital recording of images using the CCD camera.


1997 ◽  
Vol 6 (4) ◽  
pp. 34-47 ◽  
Author(s):  
Steven H. Long ◽  
Lesley B. Olswang ◽  
Julianne Brian ◽  
Philip S. Dale

This study investigated whether young children with specific expressive language impairment (SELI) learn to combine words according to general positional rules or specific, grammatic relation rules. The language of 20 children with SELI (4 females, 16 males, mean age of 33 months, mean MLU of 1.34) was sampled weekly for 9 weeks. Sixteen of these children also received treatment for two-word combinations (agent+action or possessor+possession). Two different metrics were used to determine the productivity of combinatorial utterances. One metric assessed productivity based on positional consistency alone; another assessed productivity based on positional and semantic consistency. Data were analyzed session-by-session as well as cumulatively. The results suggest that these children learned to combine words according to grammatic relation rules. Results of the session-by-session analysis were less informative than those of the cumulative analysis. For children with SELI ready to make the transition to multiword utterances, these findings support a cumulative method of data collection and a treatment approach that targets specific grammatic relation rules rather than general word combinations.


2019 ◽  
Vol 4 (2) ◽  
pp. 356-362
Author(s):  
Jennifer W. Means ◽  
Casey McCaffrey

Purpose The use of real-time recording technology for clinical instruction allows student clinicians to more easily collect data, self-reflect, and move toward independence as supervisors continue to provide continuation of supportive methods. This article discusses how the use of high-definition real-time recording, Bluetooth technology, and embedded annotation may enhance the supervisory process. It also reports results of graduate students' perception of the benefits and satisfaction with the types of technology used. Method Survey data were collected from graduate students about their use and perceived benefits of advanced technology to support supervision during their 1st clinical experience. Results Survey results indicate that students found the use of their video recordings useful for self-evaluation, data collection, and therapy preparation. The students also perceived an increase in self-confidence through the use of the Bluetooth headsets as their supervisors could provide guidance and encouragement without interrupting the flow of their therapy sessions by entering the room to redirect them. Conclusions The use of video recording technology can provide opportunities for students to review: videos of prospective clients they will be treating, their treatment videos for self-assessment purposes, and for additional data collection. Bluetooth technology provides immediate communication between the clinical educator and the student. Students reported that the result of that communication can improve their self-confidence, perceived performance, and subsequent shift toward independence.


ASHA Leader ◽  
2009 ◽  
Vol 14 (4) ◽  
pp. 7-28
Author(s):  
Jaumeiko Brown
Keyword(s):  

2008 ◽  
Vol 18 (2) ◽  
pp. 87-98 ◽  
Author(s):  
Vinciya Pandian ◽  
Thai Tran Nguyen ◽  
Marek Mirski ◽  
Nasir Islam Bhatti

Abstract The techniques of performing a tracheostomy has transformed over time. Percutaneous tracheostomy is gaining popularity over open tracheostomy given its advantages and as a result the number of bedside tracheostomies has increased necessitating the need for a Percutaneous Tracheostomy Program. The Percutaneous Tracheostomy Program at the Johns Hopkins Hospital is a comprehensive service that provides care to patients before, during, and after a tracheostomy with a multidisciplinary approach aimed at decreasing complications. Education is provided to patients, families, and health-care professionals who are involved in the management of a tracheostomy. Ongoing prospective data collection serves as a tool for Quality Assurance.


Sign in / Sign up

Export Citation Format

Share Document