scholarly journals Analysis ofBorrelia burgdorferigenotypes in patients with lyme arthritis: High frequency of ribosomal RNA intergenic spacer type 1 strains in antibiotic-refractory arthritis

2009 ◽  
Vol 60 (7) ◽  
pp. 2174-2182 ◽  
Author(s):  
Kathryn L. Jones ◽  
Gail A. McHugh ◽  
Lisa J. Glickstein ◽  
Allen C. Steere
Keyword(s):  
Ribosomal Rna ◽  
High Frequency ◽  
Intergenic Spacer ◽  
Lyme Arthritis

scholarly journals Exploring the link between C IV outflow kinematics and sublimation-temperature dust in quasars

2020 ◽  
Author(s):  
Matthew J Temple ◽  
Manda Banerji ◽  
Paul C Hewett ◽  
Amy L Rankine ◽  
Gordon T Richards
Keyword(s):  
Emission Line ◽  
High Frequency ◽  
Infrared Emission ◽  
Black Hole Mass ◽  
Sublimation Temperature ◽  
Continuum Source ◽  
Broad Emission ◽  
Line Region ◽  
Using Data

Abstract Using data from SDSS, UKIDSS and WISE, we investigate the properties of the high-frequency cutoff to the infrared emission in ≃5000 carefully selected luminous (Lbol ∼ 1047) type 1 quasars. The strength of ≃2 μm emission, corresponding to emission from the hottest ($T>1200\rm \, K$) dust in the sublimation zone surrounding the central continuum source, is observed to correlate with the blueshift of the C iv λ1550 emission line. We therefore find that objects with stronger signatures of nuclear outflows tend to have a larger covering fraction of sublimation-temperature dust. When controlling for the observed outflow strength, the hot dust covering fraction does not vary significantly across our sample as a function of luminosity, black hole mass or Eddington fraction. The correlation between the hot dust and the C iv line blueshifts, together with the lack of correlation between the hot dust and other parameters, therefore provides evidence of a link between the properties of the broad emission line region and the infrared-emitting dusty regions in quasars.

scholarly journals Effect of Hypoglycemia on Heart Rate Variability in People with Type 1 Diabetes and Impaired Awareness of Hypoglycemia

2021 ◽  
pp. 193229682110074
Author(s):  
Mats Koeneman ◽  
Marleen Olde Bekkink ◽  
Lian van Meijel ◽  
Sebastian Bredie ◽  
Bastiaan de Galan
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
High Frequency ◽  
Sympathetic Activity ◽  
Elevated Risk ◽  
Wilcoxon Rank Sum Test ◽  
Impaired Awareness ◽  
Induced Changes ◽  
Measurable Change

Background: People with impaired awareness of hypoglycemia (IAH) are at elevated risk of severe, potentially hazardous, hypoglycemia and would benefit from a device alerting to hypoglycemia. Heart rate variability (HRV) changes with hypoglycemia due to sympathetic activity. Since IAH is associated with suppressed sympathetic activity, we investigated whether hypoglycemia elicits a measurable change in HRV in patients with T1D and IAH. Method: Eligible participants underwent a modified hyperinsulinemic euglycemic hypoglycemic clamp (glucose nadir, 43.1 ± 0.90 mg/dl), while HRV was measured by a VitalConnect HealthPatch. Measurements of HRV included Root Mean Square of the Successive Differences (RMSSD) and low to high frequency (LF:HF) ratio. Wilcoxon rank-sum test was used for testing within-subject HRV changes. Results: We included 12 participants (8 female, mean age 57 ± 12 years, mean HbA1c 57 ± 5 mmol/mol (7.4 ± 0.4%)). Symptoms increased from 4.0 (1.5-7.0) at euglycemia to 7.5 (5.0-11.0) during hypoglycemia ( P = .003). In response to hypoglycemia, the LF:HF ratio and RMSSD increased when normalized for data obtained during euglycemia (both P < .01). The LF:HF ratio increased in 6 participants (50%) and declined in one other participant (8%). The RMSSD decreased in 3 (25%) and increased in 4 (33%) participants. In 2 patients, no change in HRV could be detected in response to hypoglycemia. Conclusions: This study reveals that hypoglycemia-induced changes in HRV are retained in the majority of people with T1D and IAH, and that these changes can be detected by a wearable device. Real-time HRV seems usable for detection of hypoglycemia in patients with IAH.

scholarly journals Human ribosomal RNA intergenic spacer sequence

1992 ◽  
Vol 20 (21) ◽  
pp. 5846-5846 ◽  
Author(s):  
Iris L. Gonzalez ◽  
Shuang Wu ◽  
Wen-Mei Li ◽  
Bruce A. Kuo ◽  
James E. Sylvester
Keyword(s):  
Ribosomal Rna ◽  
Intergenic Spacer

scholarly journals UNCROSS: Filtering of high-frequency cross-talk in 16S amplicon reads

2016 ◽  
Author(s):  
Robert C. Edgar
Keyword(s):  
Ribosomal Rna ◽  
Cross Talk ◽  
High Frequency ◽  
Biological Diversity ◽  
Illumina Miseq ◽  
Amplicon Sequencing ◽  
Illumina Gaiix ◽  
Microbial Metagenomics ◽  
Tumor Sequencing ◽  
Rna I

AbstractNext-generation amplicon sequencing is widely used for surveying biological diversity in applications such as microbial metagenomics, immune system repertoire analysis and targeted tumor sequencing of cancer-associated genes. In such studies, assignment of reads to incorrect samples (cross-talk) is a well-documented problem that is rarely considered in practice. By considering unexpected OTUs in artificial (mock) samples, I estimate that cross-talk occurred for ~2% of the reads in one Illumina GAIIx run and eleven Illumina MiSeq runs targeting 16S ribosomal RNA. I also describe UNCROSS, an algorithm for detecting and filtering cross-talk in OTU tables.

scholarly journals Structural and Functional Investigation and Pharmacological Mechanism of Trichosanthin, a Type 1 Ribosome-Inactivating Protein

Toxins ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 335 ◽  
Author(s):  
Wei-Wei Shi ◽  
Kam-Bo Wong ◽  
Pang-Chui Shaw
Keyword(s):  
Ribosomal Rna ◽  
Ribosome Inactivating Protein ◽  
Pharmacological Activities ◽  
Pharmacological Mechanism ◽  
Functional Studies ◽  
P Protein ◽  
Ribosomal Stalk ◽  
Functional Investigation ◽  
Ribosomal P

Trichosanthin (TCS) is an RNA N-glycosidase that depurinates adenine-4324 in the conserved α-sarcin/ricin loop (α-SRL) of rat 28 S ribosomal RNA (rRNA). TCS has only one chain, and is classified as type 1 ribosome-inactivating protein (RIP). Our structural studies revealed that TCS consists of two domains, with five conserved catalytic residues Tyr70, Tyr111, Glu160, Arg163 and Phe192 at the active cleft formed between them. We also found that the structural requirements of TCS to interact with the ribosomal stalk protein P2 C-terminal tail. The structural analyses suggest TCS attacks ribosomes by first binding to the C-terminal domain of ribosomal P protein. TCS exhibits a broad spectrum of biological and pharmacological activities including anti-tumor, anti-virus, and immune regulatory activities. This review summarizes an updated knowledge in the structural and functional studies and the mechanism of its multiple pharmacological effects.

High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion

1981 ◽  
Vol 1 (8) ◽  
pp. 743-752
Author(s):  
R M Sandri-Goldin ◽  
A L Goldin ◽  
M Levine ◽  
J C Glorioso
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Protoplast Fusion ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
High Frequency ◽  
Mammalian Cells ◽  
Frequency Transfer ◽  
Simplex Virus

The protoplast fusion technique of Schaffner (W. Schaffner, Proc. Natl. Acad. Sci. U.S.A. 77:2163-2167, 1980) has been adapted to introduce cloned herpes simplex virus genes into cultured mammalian cells. The technique involves digesting bacterial cell walls with lysozyme to produce protoplasts and then fusing the protoplasts to mammalian cells by treatment with polyethylene glycol. For monitoring transfer, protoplasts were labeled with the fluorescent dye fluorescein isothiocyanate before fusion. After fusion, greater than 50% of the mammalian cells were fluorescent, demonstrating that bacterial material was transferred with high frequency. Transfer of plasmid pBR325 occurred at frequencies of 1 to 2%, as measured by in situ hybridization. Fusion transfer of a chimeric plasmid consisting of the herpes simplex virus type 1 (strain KOS) EcoRI fragment F in pBR325 resulted in expression of some viral genomic sequences in about 5% of the mammalian cells, as detected by indirect immunofluorescence. One Ltk- cell in 300 to 500 was transformed to the TK+ phenotype after fusion with protoplasts carrying the chimeric plasmid pX1, which consists of pBR322 and the BamHI fragment coding for the herpes simplex virus type 1 thymidine kinase gene.

scholarly journals Borrelia burgdorferi activates a T helper type 1-like T cell subset in Lyme arthritis.

1991 ◽  
Vol 174 (3) ◽  
pp. 593-601 ◽  
Author(s):  
H Yssel ◽  
M C Shanafelt ◽  
C Soderberg ◽  
P V Schneider ◽  
J Anzola ◽  
...  
Keyword(s):  
T Cell ◽  
Borrelia Burgdorferi ◽  
Cell Subset ◽  
Chronic Inflammatory Disease ◽  
T Cell Response ◽  
Lyme Arthritis ◽  
T Helper ◽  
Cell Clones ◽  
T Cell Lines

18 cloned T cell lines reactive with Borrelia burgdorferi proteins, all CD3+4+8-TCR-alpha/beta+ and restricted by HLA class II proteins, were isolated from four patients with chronic Lyme arthritis. Analysis of these T cell clones indicated that the T cell response to the Lyme disease spirochete is not oligoclonally restricted; yet all produced the same pattern of lymphokines, resembling that of murine type 1 T helper cells, after antigen-specific or nonspecific stimulation. Therefore, a subset of human CD4+ T cells, with a distinct profile of lymphokine secretion, is selectively activated by the pathogen inciting this chronic inflammatory disease.

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