Reducing the glucose uptake rate inEscherichia coli affects growth rate but not protein production

2005 ◽  
Vol 90 (2) ◽  
pp. 191-200 ◽  
Author(s):  
A. Picon ◽  
M.J. Teixeira de Mattos ◽  
P.W. Postma
Keyword(s):  
Growth Rate ◽  
Glucose Uptake ◽  
Uptake Rate ◽  
Protein Production ◽  
Inescherichia Coli ◽  
Glucose Uptake Rate

scholarly journals Laboratory evolution reveals a two-dimensional rate-yield tradeoff in microbial metabolism

2018 ◽  
Author(s):  
Chuankai Cheng ◽  
Edward J. O’Brien ◽  
Douglas McCloskey ◽  
Jose Utrilla ◽  
Connor Olson ◽  
...  
Keyword(s):  
Growth Rate ◽  
Glucose Uptake ◽  
Uptake Rate ◽  
Growth Yield ◽  
Two Dimensional ◽  
Glucose Uptake Rate ◽  
E Coli ◽  
Multi Scale ◽  
Scale Modeling ◽  
Multi Scale Modeling

Growth rate and yield are fundamental features of micro-bial growth. However, we lack a mechanistic and quantita-tive understanding of the rate-yield relationship. Studies pairing computational predictions with experiments have shown the importance of maintenance energy and proteome allocation in explaining rate-yield tradeoffs and overflow metabolism. Recently, adaptive evolution experiments ofEs-cherichia colireveal a phenotypic diversity beyond what has been explained using simple models of growth rate versus yield. Here, we identify a two-dimensional rate-yield trade-off in adaptedE. colistrains where the dimensions are (A) a tradeoff between growth rate and yield and (B) a tradeoff between substrate (glucose) uptake rate and growth yield. We employ a multi-scale modeling approach, combining a previously reported coarse-grained small-scale proteome allocation model with a fine-grained genome-scale model of metabolism and gene expression (ME-model), to develop a quantitative description of the full rate-yield relationship forE. coliK-12 MG1655. The multi-scale analysis resolves the complexity of ME-model which hindered its practical use in proteome complexity analysis, and provides a mecha-nistic explanation of the two-dimensional tradeoff. Further, the analysis identifies modifications to the P/O ratio and the flux allocation between glycolysis and pentose phosphate pathway as potential mechanisms that enable the tradeoff between glucose uptake rate and growth yield. Thus, the rate-yield tradeoffs that govern microbial adaptation to new environments are more complex than previously reported, and they can be understood in mechanistic detail using a multi-scale modeling approach.

scholarly journals Antihyperglycemic and Antilipidemic Effects of the Ethanol Extract Mixture of Ligularia fischeri and Momordica charantia in Type II Diabetes-Mimicking Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-15
Author(s):  
Hyun Jin Baek ◽  
Yong Joon Jeong ◽  
Jeong Eun Kwon ◽  
Jong Sung Ra ◽  
Sung Ryul Lee ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Uptake Rate ◽  
Glucose Transporter ◽  
Synergistic Effects ◽  
Momordica Charantia ◽  
C2c12 Cells ◽  
Peroxisome Proliferator ◽  
Glucose Uptake Rate ◽  
Ligularia Fischeri

The extract of the Momordica charantia fruit (MCE) is recognized as an alternative treatment for diabetes. The extract of Ligularia fischeri leaves (LFE) is traditionally used as a folk medicine for treating inflammatory diseases in Korea as well. In this study, we investigated the synergistic effect of MCE combined with LFE on antihyperglycemic and antihyperlipidemic potentials. Based on the α-glucosidase inhibitory effect and promotion of adipocyte differentiation in the 3T3-L1 cell line, the MLM was prepared with MCE:LFE (8:2 weight:weight). MLM showed the synergistic effects in the promotion of the glucose uptake rate, suppression of dipeptidyl peptidase-4 (DPP-4) mRNA expression, upregulation of an insulin receptor substrate and glucose transporter type-4 expression, and an increase in insulin-associated signaling in C2C12 cells. In addition, the efficacy of peroxisome proliferator-activated receptor-γ agonism and glucose uptake rate by MLM supplementation was significantly enhanced in vitro. Then, the antihyperglycemic and antihyperlipidemic effects of MCE, LFE, and MLM at the dose of 50, 100, and 200 mg/kg/day (n = 6 per each group) were determined in streptozotocin (STZ)-insulted mice fed an atherogenic diet (ATH) for 4 weeks. In addition, MLM (50, 100, and 200 mg/kg/day, n = 5 per each group) was supplemented in ATH-fed db/db mice for 10 weeks. Compared with MCE or LFE alone, MLM supplementation led to a more significant reduction of glucose levels in both STZ/ATH and db/db/ATH mice as well as lowered lipid profiles in STZ/ATH mice. In addition, the stimulation of islet of Langerhans regeneration was more pronounced by MLM supplementation in both mice models. In conclusion, antihyperglycemic and antihyperlipidemic effects were strengthened by the combined extracts of L. fischeri and M. charantia (MLM) in diabetes-mimicking mice.

Effect of Flow Rate and Glucose Concentration on Glucose Uptake Rate by the Rat Limb

1977 ◽  
Vol 154 (1) ◽  
pp. 33-36 ◽  
Author(s):  
B. Grubb ◽  
J. F. Snarr
Keyword(s):  
Glucose Uptake ◽  
Flow Rate ◽  
Uptake Rate ◽  
Glucose Concentration ◽  
Glucose Uptake Rate

Glucose uptake rate as a tool to estimate hybridoma growth in a packed bed bioreactor

1999 ◽  
Vol 21 (6) ◽  
pp. 0543 ◽  
Author(s):  
M. T. A. Rodrigues ◽  
P. R. Vilaça ◽  
A. Garbuio ◽  
M. Takagi ◽  
S. Barbosa Jr. ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Uptake Rate ◽  
Packed Bed ◽  
Glucose Uptake Rate ◽  
Packed Bed Bioreactor ◽  
Hybridoma Growth

scholarly journals AMPK Phosphorylation Is Controlled by Glucose Transport Rate in a PKA-Independent Manner

2021 ◽  
Vol 22 (17) ◽  
pp. 9483
Author(s):  
Riccardo Milanesi ◽  
Farida Tripodi ◽  
Jacopo Vertemara ◽  
Renata Tisi ◽  
Paola Coccetti
Keyword(s):  
Glucose Uptake ◽  
Uptake Rate ◽  
Glucose Concentration ◽  
Inverse Correlation ◽  
Glycolytic Flux ◽  
Glucose Uptake Rate ◽  
Independent Manner ◽  
Phosphorylation State ◽  
Ampk Phosphorylation ◽  
Fructose 1,6 Bisphosphate

To achieve growth, microbial organisms must cope with stresses and adapt to the environment, exploiting the available nutrients with the highest efficiency. In Saccharomyces cerevisiae, Ras/PKA and Snf1/AMPK pathways regulate cellular metabolism according to the supply of glucose, alternatively supporting fermentation or mitochondrial respiration. Many reports have highlighted crosstalk between these two pathways, even without providing a comprehensive mechanism of regulation. Here, we show that glucose-dependent inactivation of Snf1/AMPK is independent from the Ras/PKA pathway. Decoupling glucose uptake rate from glucose concentration, we highlight a strong coordination between glycolytic metabolism and Snf1/AMPK, with an inverse correlation between Snf1/AMPK phosphorylation state and glucose uptake rate, regardless of glucose concentration in the medium. Despite fructose-1,6-bisphosphate (F1,6BP) being proposed as a glycolytic flux sensor, we demonstrate that glucose-6-phosphate (G6P), and not F1,6BP, is involved in the control of Snf1/AMPK phosphorylation state. Altogether, this study supports a model by which Snf1/AMPK senses glucose flux independently from PKA activity, and thanks to conversion of glucose into G6P.

Glycogen Metabolism and Glucose Uptake Rate of the Skeletal Muscle after Hind - limb Immobilization in Rats

1990 ◽  
Vol 25 (3) ◽  
pp. 641
Author(s):  
Joo Chul Ihn ◽  
Jong chul Ahn ◽  
Myun Whan Ahn ◽  
Chun Bae Jeon ◽  
Suck Kang Lee ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Uptake Rate ◽  
Hind Limb ◽  
Glycogen Metabolism ◽  
Glucose Uptake Rate ◽  
Limb Immobilization

Glucose Phosphoenolpyruvate Phosphotransferase Activity and Glucose Uptake Rate of Klebsiella aerogenes Growing in Chemostat Culture

Microbiology ◽  
1980 ◽  
Vol 116 (2) ◽  
pp. 305-314
Author(s):  
R. W. O'Brien ◽  
O. M. Neijssel ◽  
D. W. Tempest
Keyword(s):  
Glucose Uptake ◽  
Uptake Rate ◽  
Chemostat Culture ◽  
Klebsiella Aerogenes ◽  
Glucose Uptake Rate
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