Comparative studies on enzyme preparations and role of cell components for (R)-phenylacetylcarbinol production in a two-phase biotransformation

2006 ◽  
Vol 94 (6) ◽  
pp. 1189-1195 ◽  
Author(s):  
Gernalia Satianegara ◽  
Peter L. Rogers ◽  
Bettina Rosche
Keyword(s):  
Comparative Studies ◽  
Two Phase ◽  
Enzyme Preparations ◽  
Cell Components

Artificial RNA Editing with ADAR for Gene Therapy

2020 ◽  
Vol 20 (1) ◽  
pp. 44-54 ◽  
Author(s):  
Sonali Bhakta ◽  
Toshifumi Tsukahara
Keyword(s):  
Gene Therapy ◽  
Genetic Code ◽  
Molecular Mechanism ◽  
Rna Editing ◽  
Comparative Studies ◽  
Genetic Diseases ◽  
Adenosine Deaminases ◽  
Family Protein ◽  
Hydrolytic Deamination

Editing mutated genes is a potential way for the treatment of genetic diseases. G-to-A mutations are common in mammals and can be treated by adenosine-to-inosine (A-to-I) editing, a type of substitutional RNA editing. The molecular mechanism of A-to-I editing involves the hydrolytic deamination of adenosine to an inosine base; this reaction is mediated by RNA-specific deaminases, adenosine deaminases acting on RNA (ADARs), family protein. Here, we review recent findings regarding the application of ADARs to restoring the genetic code along with different approaches involved in the process of artificial RNA editing by ADAR. We have also addressed comparative studies of various isoforms of ADARs. Therefore, we will try to provide a detailed overview of the artificial RNA editing and the role of ADAR with a focus on the enzymatic site directed A-to-I editing.

scholarly journals Extracellular Vesicles: Novel Opportunities to Understand and Detect Neoplastic Diseases

2021 ◽  
pp. 030098582199932
Author(s):  
Laura Bongiovanni ◽  
Anneloes Andriessen ◽  
Marca H. M. Wauben ◽  
Esther N. M. Nolte-’t Hoen ◽  
Alain de Bruin
Keyword(s):  
Extracellular Vesicles ◽  
Biologically Active ◽  
Liquid Biopsies ◽  
Donor Cells ◽  
Recent Developments ◽  
Veterinary Pathology ◽  
Cell Components ◽  
Potential Applications ◽  
Intercellular Communications

With a size range from 30 to 1000 nm, extracellular vesicles (EVs) are one of the smallest cell components able to transport biologically active molecules. They mediate intercellular communications and play a fundamental role in the maintenance of tissue homeostasis and pathogenesis in several types of diseases. In particular, EVs actively contribute to cancer initiation and progression, and there is emerging understanding of their role in creation of the metastatic niche. This fact underlies the recent exponential growth in EV research, which has improved our understanding of their specific roles in disease and their potential applications in diagnosis and therapy. EVs and their biomolecular cargo reflect the state of the diseased donor cells, and can be detected in body fluids and exploited as biomarkers in cancer and other diseases. Relatively few studies have been published on EVs in the veterinary field. This review provides an overview of the features and biology of EVs as well as recent developments in EV research including techniques for isolation and analysis, and will address the way in which the EVs released by diseased tissues can be studied and exploited in the field of veterinary pathology. Uniquely, this review emphasizes the important contribution that pathologists can make to the field of EV research: pathologists can help EV scientists in studying and confirming the role of EVs and their molecular cargo in diseased tissues and as biomarkers in liquid biopsies.

scholarly journals Antioxidant and Signaling Role of Plastid-Derived Isoprenoid Quinones and Chromanols

2021 ◽  
Vol 22 (6) ◽  
pp. 2950
Author(s):  
Beatrycze Nowicka ◽  
Agnieszka Trela-Makowej ◽  
Dariusz Latowski ◽  
Kazimierz Strzalka ◽  
Renata Szymańska
Keyword(s):  
Current Knowledge ◽  
Stress Factors ◽  
Oxygenic Photosynthesis ◽  
Ros Generation ◽  
Main Site ◽  
Storage Lipids ◽  
Abiotic Stress Factors ◽  
Cell Components ◽  
And Function

Plant prenyllipids, especially isoprenoid chromanols and quinols, are very efficient low-molecular-weight lipophilic antioxidants, protecting membranes and storage lipids from reactive oxygen species (ROS). ROS are byproducts of aerobic metabolism that can damage cell components, they are also known to play a role in signaling. Plants are particularly prone to oxidative damage because oxygenic photosynthesis results in O2 formation in their green tissues. In addition, the photosynthetic electron transfer chain is an important source of ROS. Therefore, chloroplasts are the main site of ROS generation in plant cells during the light reactions of photosynthesis, and plastidic antioxidants are crucial to prevent oxidative stress, which occurs when plants are exposed to various types of stress factors, both biotic and abiotic. The increase in antioxidant content during stress acclimation is a common phenomenon. In the present review, we describe the mechanisms of ROS (singlet oxygen, superoxide, hydrogen peroxide and hydroxyl radical) production in chloroplasts in general and during exposure to abiotic stress factors, such as high light, low temperature, drought and salinity. We highlight the dual role of their presence: negative (i.e., lipid peroxidation, pigment and protein oxidation) and positive (i.e., contribution in redox-based physiological processes). Then we provide a summary of current knowledge concerning plastidic prenyllipid antioxidants belonging to isoprenoid chromanols and quinols, as well as their structure, occurrence, biosynthesis and function both in ROS detoxification and signaling.

scholarly journals Teacher Orchestration of Language and Gesture in Explaining Science Concepts in Images

2021 ◽  
Author(s):  
Thu Ngo ◽  
Len Unsworth ◽  
Michele Herrington
Keyword(s):  
High School ◽  
High School Students ◽  
Senior High School ◽  
School Students ◽  
Symbolic Representations ◽  
Different Types ◽  
Cell Components ◽  
Diagram Comprehension ◽  
Senior High School Students

AbstractStudents’ difficulties interpreting diagrams remain a concern in science education. Research about improving diagram comprehension has included few studies of teachers’ orchestration of language and gesture in explaining diagrams—and very few in senior high schools. Research with younger students and studies of research scientists’ practice indicate the significance of the interaction of teachers’ gesture and language in explaining visualisations. The strategic deployment of such teacher-focussed authoritative explanations has been observed in facilitating progression to more complex and symbolic representations in classroom work. However, the paucity of such research in senior high school leaves open the question of how these teachers use gesture and language in managing the challenges of explaining the intricate sub-microscopic and abstract visualisations senior high school students need to negotiate. In this paper, we outline existing studies of teachers’ use of gesture and language to explain complex images in senior high school and investigate how it is managed by two biology teachers with images of different types and complexity representing the activity of certain cell components in the early phase of cell duplication. Implications are drawn for foci of further research including the role of a metalanguage describing different types of visualisations and their affordances.

scholarly journals The role of interfaces on dynamic damage in two phase metals

2012 ◽  
Author(s):  
Ellen Cerreta ◽  
Saryu Fensin ◽  
Juan P. Escobedo ◽  
George Thompson Gray III ◽  
Adam Farrow ◽  
...  
Keyword(s):  
Two Phase ◽  
Dynamic Damage

Microstructure of Polycrystalline MgO Penetrated by a Silicate Liquid

1996 ◽  
Vol 2 (3) ◽  
pp. 113-128 ◽  
Author(s):  
Sundar Ramamurthy ◽  
Michael P. Mallamaci ◽  
Catherine M. Zimmerman ◽  
C. Barry Carter ◽  
Peter R. Duncombe ◽  
...  
Keyword(s):  
Grain Boundaries ◽  
Grain Growth ◽  
Glassy Phase ◽  
Silicate Liquid ◽  
Two Phase ◽  
The Matrix ◽  
Devitrification Process ◽  
Phase Mixture

Dense, polycrystalline MgO was infiltrated with monticellite (CaMgSiO4) liquid to study the penetration of liquid along the grain boundaries of MgO. Grain growth was found to be restricted with increasing amounts of liquid. The inter-granular regions were generally found to be comprised of a two-phase mixture: crystalline monticellite and a glassy phase rich in the impurities present in the starting MgO material. MgO grains act as seeding agents for the crystallization of monticellite. The location and composition of the glassy phase with respect to the MgO grains emphasizes the role of intergranular liquid during the devitrification process in “snowplowing” impurities present in the matrix.

Role of activation energies of individual phases in two-phase range on constitutive equation of Zr–2.5Nb–0.5Cu alloy

2017 ◽  
Vol 27 (1) ◽  
pp. 172-183 ◽  
Author(s):  
K.K. SAXENA ◽  
S.K. JHA ◽  
V. PANCHOLI ◽  
G.P. CHAUDHARI ◽  
D. SRIVASTAVA ◽  
...  
Keyword(s):  
Constitutive Equation ◽  
Activation Energies ◽  
Two Phase ◽  
Phase Range

Anomalies of continental precipitation associated with El Niño Southern Oscillation: the role of moisture contribution from oceanic and terrestrial sources 

2021 ◽  
Author(s):  
Rogert Sorí ◽  
Raquel Nieto ◽  
Margarida L.R. Liberato ◽  
Luis Gimeno
Keyword(s):  
South America ◽  
Southern Oscillation ◽  
The United States ◽  
Precipitation Pattern ◽  
Two Phase ◽  
The North ◽  
Terrestrial Origin ◽  
Precipitation Anomalies ◽  
June July

<p>The regional and global precipitation pattern is highly modulated by the influence of El Niño Southern Oscillation (ENSO), which is considered the most important mode of climate variability on the planet. In this study was investigated the asymmetry of the continental precipitation anomalies during El Niño and La Niña. To do it, a Lagrangian approach already validated was used to determine the proportion of the total Lagrangian precipitation that is of oceanic and terrestrial origin. During both, El Niño and La Niña, the Lagrangian precipitation in regions such as the northeast of South America, the east and west coast of North America, Europe, the south of West Africa, Southeast Asia, and Oceania is generally determined by the oceanic component of the precipitation, while that from terrestrial origin provides a major percentage of the average Lagrangian precipitation towards the interior of the continents. The role of the moisture contribution to precipitation from terrestrial and oceanic origin was evaluated in regions with statistically significant precipitation anomalies during El Niño and La Niña. Two-phase asymmetric behavior of the precipitation was found in regions such the northeast of South America, South Africa, the north of Mexico, and southeast of the United States, etc. principally for December-January-February and June-July-August. For some of these regions was also calculated the anomalies of the precipitation from other datasets to confirm the changes. Besides, for these regions was calculated the anomaly of the Lagrangian precipitation, which agrees in all the cases with the precipitation change. For these regions, it was determined which component of the Lagrangian precipitation, whether oceanic or terrestrial, controlled the precipitation anomalies. A schematic figure represents the extent of the most important seasonal oceanic and terrestrial sources for each subregion during El Niño and La Niña.</p>

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