scholarly journals Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations

2011 ◽  
Vol 109 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Sebastian L. Riedel ◽  
Johannes Bader ◽  
Christopher J. Brigham ◽  
Charles F. Budde ◽  
Zainal Abidin Mohd Yusof ◽  
...  
Keyword(s):  
Cell Density ◽  
Palm Oil ◽  
Ralstonia Eutropha ◽  
High Cell Density ◽  
High Cell

scholarly journals In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy

2019 ◽  
Vol 6 (3) ◽  
pp. 85 ◽  
Author(s):  
Björn Gutschmann ◽  
Thomas Schiewe ◽  
Manon T.H. Weiske ◽  
Peter Neubauer ◽  
Roland Hass ◽  
...  
Keyword(s):  
Cell Density ◽  
Ralstonia Eutropha ◽  
High Cell Density ◽  
Process Analytical Technology ◽  
Density Wave ◽  
Dry Weight ◽  
Photon Density ◽  
High Cell ◽  
Plant Oil ◽  
Pha Production

Polyhydroxyalkanoates (PHAs) are biodegradable plastic-like materials with versatile properties. Plant oils are excellent carbon sources for a cost-effective PHA production, due to their high carbon content, large availability, and comparatively low prices. Additionally, efficient process development and control is required for competitive PHA production, which can be facilitated by on-line or in-line monitoring devices. To this end, we have evaluated photon density wave (PDW) spectroscopy as a new process analytical technology for Ralstonia eutropha (Cupriavidus necator) H16 plant oil cultivations producing polyhydroxybutyrate (PHB) as an intracellular polymer. PDW spectroscopy was used for in-line recording of the reduced scattering coefficient µs’ and the absorption coefficient µa at 638 nm. A correlation of µs’ with the cell dry weight (CDW) and µa with the residual cell dry weight (RCDW) was observed during growth, PHB accumulation, and PHB degradation phases in batch and pulse feed cultivations. The correlation was used to predict CDW, RCDW, and PHB formation in a high-cell-density fed-batch cultivation with a productivity of 1.65 gPHB·L−1·h−1 and a final biomass of 106 g·L−1 containing 73 wt% PHB. The new method applied in this study allows in-line monitoring of CDW, RCDW, and PHA formation.

Modeling of poly(3-hydroxybutyrate) production by high cell density fed-batch culture of Ralstonia eutropha

2007 ◽  
Vol 12 (4) ◽  
pp. 417-423 ◽  
Author(s):  
Longan Shang ◽  
Dai Di Fan ◽  
Moon Il Kim ◽  
Jin-dal-rae Choi ◽  
Ho Nam Chang
Keyword(s):  
Cell Density ◽  
Batch Culture ◽  
Ralstonia Eutropha ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Fed Batch Culture

scholarly journals A Novel High-Cell-Density Protein Expression System Based on Ralstonia eutropha

2002 ◽  
Vol 68 (12) ◽  
pp. 5925-5932 ◽  
Author(s):  
Sriram Srinivasan ◽  
Gavin C. Barnard ◽  
Tillman U. Gerngross
Keyword(s):  
Protein Expression ◽  
Cell Density ◽  
Ralstonia Eutropha ◽  
High Cell Density ◽  
Expression System ◽  
High Cell ◽  
Protein Expression System ◽  
Inclusion Body Formation ◽  
High Density Cell ◽  
High Level

ABSTRACT We describe the development of a novel protein expression system based on the industrial fermentation organism Ralstonia eutropha (formerly known as Alcaligenes eutrophus) NCIMB 40124. This new system overcomes some of the shortcomings of traditional Escherichia coli-based protein expression systems, particularly the propensity of such systems to form inclusion bodies during high-level expression. Using a proteomics approach, we identified promoters that can be induced by simple process parameters or medium compositions in high-density cell culture or shake flasks, respectively. By combining newly developed molecular biological tools with a high-cell-density fermentation process, we were able to produce high levels (>1 g/liter) of soluble, active organophosphohydrolase, a model enzyme prone to inclusion body formation in E. coli.

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