New suspension culture system (Mini-AR)

1975 ◽  
Vol 17 (10) ◽  
pp. 1467-1471 ◽  
Author(s):  
Roselynn Dynesius ◽  
Robert D. Lange ◽  
Arsev H. Eraslan ◽  
William T. Snyder
Keyword(s):  
Suspension Culture ◽  
Culture System

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

A Novel Continuous Suspension Culture System for Hematopoietic Cells

2008 ◽  
pp. 285-287
Author(s):  
S. Schmidt ◽  
N. Jelinek ◽  
U. Hilbert ◽  
S. Thoma ◽  
C. Wandrey ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Culture System ◽  
Hematopoietic Cells

Establishment and evaluation of the suspension culture system for umbilical cord-derived mesenchymal stromal cells

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S39
Author(s):  
H. Hasegawa ◽  
A. Takahashi ◽  
T. Shimazu ◽  
A. Hori ◽  
T. Furuno ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Culture System

CNTO328 Abrogates Glucocorticoid (GC) Resistance Conferred by Interleukin (IL)-6 and the Bone Marrow Microenvironment in Multiple Myeloma (MM).

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3473-3473
Author(s):  
Peter M. Voorhees ◽  
George W. Small ◽  
Deborah J. Kuhn ◽  
Qing Chen ◽  
Sally A. Hunsucker ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Suspension Culture ◽  
Cell Viability ◽  
Stromal Cells ◽  
Culture System ◽  
Lymphoblastic Leukemia ◽  
Critical Role ◽  
Bone Marrow Microenvironment ◽  
Bone Marrow Stroma ◽  
Marrow Stroma

Abstract Given the critical role that IL-6 plays in MM cell proliferation, survival, and resistance to GCs, we evaluated the ability of CNTO328, a chimeric monoclonal IL-6 neutralizing antibody, to overcome GC resistance in cell line models of human MM. In the presence of IL-6, the MM cell lines ANBL-6 and KAS-6 were resistant to the cytotoxic activity of dexamethasone (Dex) as assessed by cell viability assays both in suspension culture and in the context of patient-derived stromal cells. Resistance to dexamethasone was readily reversed by CNTO328, but not an isotype control antibody, in suspension culture. For example, in the case of the ANBL-6 model, viability was reduced by 12% with CNTO328 alone, 8% with Dex, but 74% with the combination, consistent with a synergistic interaction Given the ability of other growth factors in the bone marrow microenvironment to confer GC resistance in preclinical models of MM, we evaluated the activity of the CNTO328 and Dex combination in ANBL-6 and KAS-6 cells using a physiologically-relevant MM cell/patient-derived bone marrow stromal cell co-culture system. Importantly, bone marrow stromal cells rendered ANBL-6 and KAS-6 cells resistant to Dex in cell viability assays, and CNTO328 was able to reestablish Dex sensitivity, thus confirming a central role of IL-6 in bone marrow stroma-mediated GC resistance. Furthermore, treatment of ANBL-6 and KAS-6 cells with Dex alone did not induce apoptosis in this co-culture system, whereas the combination of CNTO328 and Dex led to a synergistic induction of apoptosis. In KAS-6 cells, IL-6-mediated Dex resistance was not overcome using pharmacologic inhibitors to p38, PI-3 kinase, mTor or MEK, suggesting that other IL-6 signaling pathways are likely involved. In contrast, the mTor inhibitor rapamycin was capable of sensitizing ANBL-6 cells to Dex in the presence of IL-6, suggesting that this pathway may be relevant to IL-6-mediated GC resistance in these cells. Induction of the pro-apoptotic Bcl-2 family member, Bim, has been shown to play an important role in GC-mediated cell death in lymphocytes as well as preclinical lymphoma and acute lymphoblastic leukemia models. Interestingly, although treatment of ANBL-6 cells in the presence of IL-6 with either CNTO328 or dexamethasone did not lead to induction of Bim, the combination led to a 3.3-fold increase in its expression. Taken together, the above data demonstrate that inhibition of IL-6 signaling with CNTO328 can effectively overcome IL-6-mediated GC resistance even in the presence of bone marrow stroma, and provide a compelling rationale for translation of this combination into clinical trials for patients suffering from MM. Furthermore, we show that the ability of CNTO328 to overcome GC resistance may be mediated in part by its ability to reverse IL-6-mediated repression of GC-induced Bim expression. Studies evaluating the relevance of Bim modulation in IL-6-mediated GC resistance and the molecular pathways that mediate this effect are on-going.

Accumulation and tolerance of Cr and Pb using a cell suspension culture system of Jatropha curcas

2014 ◽  
Vol 120 (1) ◽  
pp. 221-228 ◽  
Author(s):  
A. Bernabé-Antonio ◽  
L. Álvarez ◽  
L. Buendía-González ◽  
A. Maldonado-Magaña ◽  
F. Cruz-Sosa
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Jatropha Curcas ◽  
Culture System ◽  
A Cell

Scaling up a chemically-defined aggregate-based suspension culture system for neural commitment of human pluripotent stem cells

2016 ◽  
Vol 11 (12) ◽  
pp. 1628-1638 ◽  
Author(s):  
Cláudia C. Miranda ◽  
Tiago G. Fernandes ◽  
M. Margarida Diogo ◽  
Joaquim M.S. Cabral
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Culture System ◽  
Scaling Up ◽  
Human Pluripotent Stem Cells

scholarly journals Full Transcriptome Analysis of Callus Suspension Culture System of Bletilla striata

2020 ◽  
Vol 11 ◽  
Author(s):  
Lin Li ◽  
Houbo Liu ◽  
Weie Wen ◽  
Ceyin Huang ◽  
Xiaomei Li ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Transcriptome Analysis ◽  
Culture System ◽  
Bletilla Striata
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