Effect of operating parameters on specific production rate of a cloned-gene product and performance of recombinant fermentation process

According to the results of the single temperature on Monascus anka mutant growth and monascus yellow pigments production in this paper, two stages in the fermentation temperature control strategy has been operated as following: the temperature was 34°C to improve the Monascus growth before culture 48 hour and then 32°C was carried out to improve theItalic text yellow pigments production. The results demonstrated that two stages temperature control at 96 hours, the yield of monascus yellow pigments can arrive 164.51 OD units, it was 180.35%, 2165.48%, 107.66%, 5.76%, 33.23%, 36.74% higher than that of 26°C, 28°C, 30°C, 32°C, 34°C, 36°C fermentation, respectively. The maximum specific growth rate(µ) and the maximum specific production rate of yellow pigments( qy) could arrive 0.0528 h-1 and 0.404 OD.g-1.h-1, and it was 5.30% and 3.22% higher than that of 34°C and 32°C, respectively. The specific production rate of yellow pigments was varied from 0.2 OD.g-1.h-1 to 0.4 OD.g-1.h-1 in whole fermentation. Two stage temperature control strategy could improve the yellow pigments production, increase µ and and short the culture time.

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Photosynthetic carbon metabolism of size-fractionated phytoplankton during an experimental bloom in marine microcosms

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400036560 ◽

1990 ◽

Vol 70 (3) ◽

pp. 531-543 ◽

Cited By ~ 16

Author(s):

I. De Madariaga ◽

E. Fernandez

Keyword(s):

Production Rate ◽

Growth Rates ◽

Size Fraction ◽

Field Studies ◽

Exponential Growth Phase ◽

Specific Production ◽

Specific Production Rate ◽

Size Fractions ◽

Close Relationship ◽

Metabolite Synthesis

Water samples, taken from station El in the English Channel, were incubated in 12–1 bottles and monitored for nine days. The distribution pattern of photosynthetically incorporated 14C was followed in three different size-fractions (0·2–2 μm, 2–10 μm and >10 μm) during the development of a bloom.A rapid increase in chlorophyll was observed just after enclosure, the highest values being reached by day 5, when a mixed community of diatoms and small flagellates formed the bulk of phytoplankton biomass. As the bloom developed, nutrients were depleted and, consequently, a decay of the phytoplankton populations occurred. Growth rates, estimated as chlorophyll-specific production rate, were higher for the largest size-fraction (0·53 d−1) than for the intermediate and smallest ones (0·32 d−1 for both).Throughout the bloom, the proportions of 14C incorporated into protein and low molecular weight metabolites (LMWM) were small, whereas the synthesis of storage products appeared to be enhanced. Carbon fixed into protein was higher during the exponential growth phase in all size-fractions, and incorporation into LMWM increased sharply when the bloom declined. Storage product synthesis varied between size-fractions.A close relationship was observed between growth rates, estimated as chlorophyll-specific production rate, and the protein/metabolite synthesis ratio (r2=0·90; n=24). These results suggest that, within the range of environmental variation we studied, this ratio would be a good estimator of growth rate for field studies.

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Effects of pH and Aeration on the Production of Docosahexaenoic Acid by Thraustochytrium aureum in Controlled Batch Fermentor Cultures

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.50 ◽

2011 ◽

Vol 183-185 ◽

pp. 50-54

Author(s):

Ge Yang ◽

Liu Yan ◽

Ming Gao

Keyword(s):

Docosahexaenoic Acid ◽

Production Rate ◽

Structural Component ◽

Linseed Oil ◽

Specific Production ◽

Oil Consumption ◽

Specific Production Rate ◽

Thraustochytrium Aureum ◽

Effects Of Ph ◽

Maltose Utilization

Docosahexaenoic acid (DHA. C22:6 ω-3) is an important structural component of neural and retinal tissues .The DHA yield was highest at pH5.5. Glucose and maltose utilization were similar as a function of pH, linseed oil consumption increased at pH 5.5, indicating that the formation of DHA from linseed oil at pH5.5 was of increased importance. Increased aeration resulted in doubling of T. aureum cell dry weights, increasing DHA yields and increasing in the maximum DHA -specific production rate .

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Effect of changes in continuous carboxylate feeding on the specific production rate of butanol using Clostridium saccharoperbutylacetonicum

Bioresource Technology ◽

10.1016/j.biortech.2021.125057 ◽

2021 ◽

Vol 332 ◽

pp. 125057

Author(s):

Florian Gattermayr ◽

Christoph Herwig ◽

Viktoria Leitner

Keyword(s):

Production Rate ◽

Specific Production ◽

Specific Production Rate

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Synthesis and Characterization of Novel Bio-Chiral Dopants Obtained from Bio-Betulin Produced by a Fermentation Process

Crystals ◽

10.3390/cryst11070785 ◽

2021 ◽

Vol 11 (7) ◽

pp. 785

Author(s):

Veridiana G. Guimarães ◽

Anastasiia Svanidze ◽

Tianyi Guo ◽

Pawan Nepal ◽

Robert J. Twieg ◽

...

Keyword(s):

Liquid Crystals ◽

Natural Product ◽

Fermentation Process ◽

Synthesis And Characterization ◽

Cholesteric Liquid Crystals ◽

And Performance ◽

Chiral Dopants

Cholesteric liquid crystals are frequently produced by the addition of chiral dopants to achiral nematic hosts. We report here the synthesis and performance of chiral dopants obtained from bio-betulin produced by a fermentation process. An important aspect of this work is to point out that the fermentation process used to obtain the starting materials is much easier and cheaper when carried out in large volumes than isolating it from the natural product. The performance of the dopants obtained from bio-betulin is indistinguishable from those obtained from commercially available synthetic betulin.

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