A Temperature-Shift Strategy in Batch Monascus Yellow Pigments Fermentation

2012 ◽  
Vol 550-553 ◽  
pp. 1327-1335
Author(s):  
Bo Zhou ◽  
Jing Jing Kang

According to the results of the single temperature on Monascus anka mutant growth and monascus yellow pigments production in this paper, two stages in the fermentation temperature control strategy has been operated as following: the temperature was 34°C to improve the Monascus growth before culture 48 hour and then 32°C was carried out to improve theItalic text yellow pigments production. The results demonstrated that two stages temperature control at 96 hours, the yield of monascus yellow pigments can arrive 164.51 OD units, it was 180.35%, 2165.48%, 107.66%, 5.76%, 33.23%, 36.74% higher than that of 26°C, 28°C, 30°C, 32°C, 34°C, 36°C fermentation, respectively. The maximum specific growth rate(µ) and the maximum specific production rate of yellow pigments( qy) could arrive 0.0528 h-1 and 0.404 OD.g-1.h-1, and it was 5.30% and 3.22% higher than that of 34°C and 32°C, respectively. The specific production rate of yellow pigments was varied from 0.2 OD.g-1.h-1 to 0.4 OD.g-1.h-1 in whole fermentation. Two stage temperature control strategy could improve the yellow pigments production, increase µ and and short the culture time.

1996 ◽  
Vol 31 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Anissa Lounès ◽  
Ahmed Lebrihi ◽  
Chouki Benslimane ◽  
Gérard Lefebvre ◽  
Pierre Germain

Author(s):  
I. De Madariaga ◽  
E. Fernandez

Water samples, taken from station El in the English Channel, were incubated in 12–1 bottles and monitored for nine days. The distribution pattern of photosynthetically incorporated 14C was followed in three different size-fractions (0·2–2 μm, 2–10 μm and >10 μm) during the development of a bloom.A rapid increase in chlorophyll was observed just after enclosure, the highest values being reached by day 5, when a mixed community of diatoms and small flagellates formed the bulk of phytoplankton biomass. As the bloom developed, nutrients were depleted and, consequently, a decay of the phytoplankton populations occurred. Growth rates, estimated as chlorophyll-specific production rate, were higher for the largest size-fraction (0·53 d−1) than for the intermediate and smallest ones (0·32 d−1 for both).Throughout the bloom, the proportions of 14C incorporated into protein and low molecular weight metabolites (LMWM) were small, whereas the synthesis of storage products appeared to be enhanced. Carbon fixed into protein was higher during the exponential growth phase in all size-fractions, and incorporation into LMWM increased sharply when the bloom declined. Storage product synthesis varied between size-fractions.A close relationship was observed between growth rates, estimated as chlorophyll-specific production rate, and the protein/metabolite synthesis ratio (r2=0·90; n=24). These results suggest that, within the range of environmental variation we studied, this ratio would be a good estimator of growth rate for field studies.


2011 ◽  
Vol 183-185 ◽  
pp. 50-54
Author(s):  
Ge Yang ◽  
Liu Yan ◽  
Ming Gao

Docosahexaenoic acid (DHA. C22:6 ω-3) is an important structural component of neural and retinal tissues .The DHA yield was highest at pH5.5. Glucose and maltose utilization were similar as a function of pH, linseed oil consumption increased at pH 5.5, indicating that the formation of DHA from linseed oil at pH5.5 was of increased importance. Increased aeration resulted in doubling of T. aureum cell dry weights, increasing DHA yields and increasing in the maximum DHA -specific production rate .


TAPPI Journal ◽  
2018 ◽  
Vol 17 (05) ◽  
pp. 295-305
Author(s):  
Wesley Gilbert ◽  
Ivan Trush ◽  
Bruce Allison ◽  
Randy Reimer ◽  
Howard Mason

Normal practice in continuous digester operation is to set the production rate through the chip meter speed. This speed is seldom, if ever, adjusted except to change production, and most of the other digester inputs are ratioed to it. The inherent assumption is that constant chip meter speed equates to constant dry mass flow of chips. This is seldom, if ever, true. As a result, the actual production rate, effective alkali (EA)-to-wood and liquor-to-wood ratios may vary substantially from assumed values. This increases process variability and decreases profits. In this report, a new continuous digester production rate control strategy is developed that addresses this shortcoming. A new noncontacting near infrared–based chip moisture sensor is combined with the existing weightometer signal to estimate the actual dry chip mass feedrate entering the digester. The estimated feedrate is then used to implement a novel feedback control strategy that adjusts the chip meter speed to maintain the dry chip feedrate at the target value. The report details the results of applying the new measurements and control strategy to a dual vessel continuous digester.


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