Specific production rate of VHH antibody fragments by Saccharomyces cerevisiae is correlated with growth rate, independent of nutrient limitation

2005 ◽  
Vol 118 (3) ◽  
pp. 270-277 ◽  
Author(s):  
Yvonne E. Thomassen ◽  
Arie J. Verkleij ◽  
Johannes Boonstra ◽  
C. Theo Verrips
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Nutrient Limitation ◽  
Production Rate ◽  
Antibody Fragments ◽  
Specific Production ◽  
Specific Production Rate

Effect of nitrogen/carbon ratio on the specific production rate of spiramycin by Streptomyces ambofaciens

1996 ◽  
Vol 31 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Anissa Lounès ◽  
Ahmed Lebrihi ◽  
Chouki Benslimane ◽  
Gérard Lefebvre ◽  
Pierre Germain
Keyword(s):  
Production Rate ◽  
Specific Production ◽  
Specific Production Rate ◽  
Streptomyces Ambofaciens

A Temperature-Shift Strategy in Batch Monascus Yellow Pigments Fermentation

2012 ◽  
Vol 550-553 ◽  
pp. 1327-1335
Author(s):  
Bo Zhou ◽  
Jing Jing Kang
Keyword(s):  
Production Rate ◽  
Temperature Control ◽  
Control Strategy ◽  
Temperature Shift ◽  
Specific Production ◽  
Specific Production Rate ◽  
Single Temperature ◽  
Yellow Pigments ◽  
Two Stages ◽  
Monascus Yellow Pigments

According to the results of the single temperature on Monascus anka mutant growth and monascus yellow pigments production in this paper, two stages in the fermentation temperature control strategy has been operated as following: the temperature was 34°C to improve the Monascus growth before culture 48 hour and then 32°C was carried out to improve theItalic text yellow pigments production. The results demonstrated that two stages temperature control at 96 hours, the yield of monascus yellow pigments can arrive 164.51 OD units, it was 180.35%, 2165.48%, 107.66%, 5.76%, 33.23%, 36.74% higher than that of 26°C, 28°C, 30°C, 32°C, 34°C, 36°C fermentation, respectively. The maximum specific growth rate(µ) and the maximum specific production rate of yellow pigments( qy) could arrive 0.0528 h-1 and 0.404 OD.g-1.h-1, and it was 5.30% and 3.22% higher than that of 34°C and 32°C, respectively. The specific production rate of yellow pigments was varied from 0.2 OD.g-1.h-1 to 0.4 OD.g-1.h-1 in whole fermentation. Two stage temperature control strategy could improve the yellow pigments production, increase µ and and short the culture time.

Photosynthetic carbon metabolism of size-fractionated phytoplankton during an experimental bloom in marine microcosms

1990 ◽  
Vol 70 (3) ◽  
pp. 531-543 ◽  
Author(s):  
I. De Madariaga ◽  
E. Fernandez
Keyword(s):  
Production Rate ◽  
Growth Rates ◽  
Size Fraction ◽  
Field Studies ◽  
Exponential Growth Phase ◽  
Specific Production ◽  
Specific Production Rate ◽  
Size Fractions ◽  
Close Relationship ◽  
Metabolite Synthesis

Water samples, taken from station El in the English Channel, were incubated in 12–1 bottles and monitored for nine days. The distribution pattern of photosynthetically incorporated 14C was followed in three different size-fractions (0·2–2 μm, 2–10 μm and >10 μm) during the development of a bloom.A rapid increase in chlorophyll was observed just after enclosure, the highest values being reached by day 5, when a mixed community of diatoms and small flagellates formed the bulk of phytoplankton biomass. As the bloom developed, nutrients were depleted and, consequently, a decay of the phytoplankton populations occurred. Growth rates, estimated as chlorophyll-specific production rate, were higher for the largest size-fraction (0·53 d−1) than for the intermediate and smallest ones (0·32 d−1 for both).Throughout the bloom, the proportions of 14C incorporated into protein and low molecular weight metabolites (LMWM) were small, whereas the synthesis of storage products appeared to be enhanced. Carbon fixed into protein was higher during the exponential growth phase in all size-fractions, and incorporation into LMWM increased sharply when the bloom declined. Storage product synthesis varied between size-fractions.A close relationship was observed between growth rates, estimated as chlorophyll-specific production rate, and the protein/metabolite synthesis ratio (r2=0·90; n=24). These results suggest that, within the range of environmental variation we studied, this ratio would be a good estimator of growth rate for field studies.

Effects of pH and Aeration on the Production of Docosahexaenoic Acid by Thraustochytrium aureum in Controlled Batch Fermentor Cultures

2011 ◽  
Vol 183-185 ◽  
pp. 50-54
Author(s):  
Ge Yang ◽  
Liu Yan ◽  
Ming Gao
Keyword(s):  
Docosahexaenoic Acid ◽  
Production Rate ◽  
Structural Component ◽  
Linseed Oil ◽  
Specific Production ◽  
Oil Consumption ◽  
Specific Production Rate ◽  
Thraustochytrium Aureum ◽  
Effects Of Ph ◽  
Maltose Utilization

Docosahexaenoic acid (DHA. C22:6 ω-3) is an important structural component of neural and retinal tissues .The DHA yield was highest at pH5.5. Glucose and maltose utilization were similar as a function of pH, linseed oil consumption increased at pH 5.5, indicating that the formation of DHA from linseed oil at pH5.5 was of increased importance. Increased aeration resulted in doubling of T. aureum cell dry weights, increasing DHA yields and increasing in the maximum DHA -specific production rate .

scholarly journals Maintenance of Mitochondrial Morphology Is Linked to Maintenance of the Mitochondrial Genome in Saccharomyces cerevisiae

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1147-1156 ◽  
Author(s):  
Theodor Hanekamp ◽  
Mary K Thorsness ◽  
Indrani Rebbapragada ◽  
Elizabeth M Fisher ◽  
Corrine Seebart ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Mitochondrial Dna ◽  
Carbon Sources ◽  
Mitochondrial Morphology ◽  
Yeast Saccharomyces Cerevisiae ◽  
Slow Growth Rate ◽  
Dna Migration ◽  
Mitochondrial Dna Stability ◽  
Extragenic Suppressors

Abstract In the yeast Saccharomyces cerevisiae, certain mutant alleles of YME4, YME6, and MDM10 cause an increased rate of mitochondrial DNA migration to the nucleus, carbon-source-dependent alterations in mitochondrial morphology, and increased rates of mitochondrial DNA loss. While single mutants grow on media requiring mitochondrial respiration, any pairwise combination of these mutations causes a respiratory-deficient phenotype. This double-mutant phenotype allowed cloning of YME6, which is identical to MMM1 and encodes an outer mitochondrial membrane protein essential for maintaining normal mitochondrial morphology. Yeast strains bearing null mutations of MMM1 have altered mitochondrial morphology and a slow growth rate on all carbon sources and quantitatively lack mitochondrial DNA. Extragenic suppressors of MMM1 deletion mutants partially restore mitochondrial morphology to the wild-type state and have a corresponding increase in growth rate and mitochondrial DNA stability. A dominant suppressor also suppresses the phenotypes caused by a point mutation in MMM1, as well as by specific mutations in YME4 and MDM10.

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