scholarly journals LacZ‐reporter mapping of Dlx5 / 6 expression and genoarchitectural analysis of the postnatal mouse prethalamus

2020 ◽  
Vol 529 (2) ◽  
pp. 367-420 ◽  
Author(s):  
Luis Puelles ◽  
Carmen Diaz ◽  
Thorsten Stühmer ◽  
José L. Ferran ◽  
Margaret Martínez‐de la Torre ◽  
...  
Keyword(s):  
Lacz Reporter

scholarly journals Interactions of Drosophila Ultrabithorax Regulatory Regions With Native and Foreign Promoters

Genetics ◽  
1997 ◽  
Vol 145 (1) ◽  
pp. 123-137 ◽  
Author(s):  
Fernando Casares ◽  
Welcome Bender ◽  
John Merriam ◽  
Ernesto Sánchez-Herrero
Keyword(s):  
P Element ◽  
Lacz Gene ◽  
Bithorax Complex ◽  
Lacz Reporter ◽  
Normal Pattern ◽  
Lacz Reporter Gene ◽  
Regulatory Regions ◽  
Long Range Interactions ◽  
In Trans ◽  
Dependent Interaction

The Ultrabithorax (Ubx) gene of the Drosophila bithorax complex is required to specify parasegments 5 and 6. Two P-element “enhancer traps” have been recovered within the locus that contain the bacterial lacZ gene under the control of the P-element promoter. The P insertion that is closer to the Ubx promoter expresses lucZ in a pattern similar to that of the normal Ubx gene, but also in parasegment 4 during embryonic development. Two deletions have been recovered that remove the normal Ubx promoter plus several kilobases on either side, but retain the lacZ reporter gene. The lacZ patterns from the deletion derivatives closely match the normal pattern of Ubx expression in late embryos and imaginal discs. The lacZ genes in the deletion derivatives are also negatively regulated by Ubx and activated in trans by Contrabithorax mutations, again like the normal Ubx gene. Thus, the deleted regions, including several kilobases around the Ubx promoter, are not required for long range interactions with Ubx regulatory regions. The deletion derivatives also stimulate transvection, a pairing-dependent interaction with the Ubx promoter on the homologous chromosome.

scholarly journals An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (5) ◽  
Author(s):  
Xiaopeng Shen ◽  
Feng Xu ◽  
Shen Wu ◽  
Meng Li ◽  
Jingyi Zhang ◽  
...  
Keyword(s):  
Staining Method ◽  
Mouse Embryos ◽  
Lacz Reporter

Promoter Assessment in Hansenula polymorpha Using a lacZ Reporter Gene

2003 ◽  
pp. 101-115
Author(s):  
Manfred Suckow ◽  
Martina Kutzner ◽  
Carsten Amuel ◽  
Cornelis P. Hollenberg ◽  
Gerd Gellissen
Keyword(s):  
Reporter Gene ◽  
Hansenula Polymorpha ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

Nucleosomal location of the STE6 TATA box and Mat alpha 2p-mediated repression

1994 ◽  
Vol 14 (6) ◽  
pp. 4002-4010
Author(s):  
H G Patterton ◽  
R T Simpson
Keyword(s):  
Reporter Gene ◽  
Tata Box ◽  
Chromatin Domain ◽  
Specific Gene ◽  
Multicopy Plasmid ◽  
Alpha Cells ◽  
Lacz Reporter ◽  
Lacz Reporter Gene ◽  
Histone Octamer ◽  
Repression Domain

It has been proposed that yeast MATa cell-specific genes are repressed in MAT alpha cells by the Mat alpha 2p repressor-directed placement of a nucleosome in a position that incorporates the TATA box of the MATa-specific gene close to the nucleosomal pseudodyad. In this study, we address this proposal directly with a series of plasmids designed to place the MATa-specific STE6 TATA box at different locations in a nucleosome and in the internucleosomal linker. These plasmids contain different lengths of synthetic random DNA between the Mat alpha 2p operator and the TATA box of the STE6 promoter, which is located upstream of a lacZ reporter gene in a multicopy plasmid. We show that in MAT alpha cells, a nucleosome is retained in an identical translational frame relative to the Mat alpha 2p operator in all the constructs investigated, irrespective of the sequence of the DNA wrapped onto the histone octamer. This result shows that the nucleosomal organization of the STE6 promoter in MAT alpha cells is not conferred by the sequence of the promoter itself. No expression of the lacZ reporter gene was detectable in MAT alpha cells in any of the constructs, even with the TATA box located in a short internucleosomal linker. These data indicate that repression of MATa-specific genes in MAT alpha cells does not require the precise translational placement of the TATA box close to the nucleosomal pseudodyad; the gene remains repressed when the TATA box is located within the investigated 250-bp region in the organized chromatin domain abutting the Mat alpha 2p operator in MAT alpha cells and may remain repressed with the TATA box located anywhere within this organized repression domain.

The C. elegans neuronally expressed homeobox gene ceh-10 is closely related to genes expressed in the vertebrate eye

Development ◽  
1995 ◽  
Vol 121 (5) ◽  
pp. 1253-1262 ◽  
Author(s):  
P.C. Svendsen ◽  
J.D. McGhee
Keyword(s):  
Sensory Cell ◽  
Homeobox Gene ◽  
Bipolar Cells ◽  
Lacz Reporter ◽  
Nematode Caenorhabditis Elegans ◽  
Lacz Reporter Gene ◽  
Vertebrate Retina ◽  
C Elegans ◽  
A Cell ◽  
Vertebrate Eye

We describe the homeobox gene ceh-10 from the nematode Caenorhabditis elegans. The homeodomain of ceh-10 is closely related to the homeodomains of two genes recently cloned from the vertebrate retina, Chx10 from mice and Vsx-1 from goldfish. We show that the sequence conservation extends well beyond the homeodomain and includes a region (named the CVC domain) of roughly 60 amino acids immediately C-terminal to the homeodomain. As assayed in transgenic worms, the promoter region of ceh-10 directs expression of a lacZ reporter gene to a small number of neurons. We draw a parallel between the bipolar cells of the inner nuclear layer of the vertebrate retina, which express Chx10 and Vsx-1, and an interneuron in C. elegans called AIY, which expresses ceh-10. AIY receives synaptic input from a sensory cell, just as do bipolar cells of the vertebrate retina. In C. elegans, the sensory cell AFD is not known to be photosensitive but is known to be thermosensitive; moreover, a cell with similar position in the amphids of other nematodes has been suggested indeed to be photosensitive. Our results emphasize the highly conserved nature of sensory regulatory mechanisms and suggest one way in which photosensitive organelles might have originated in evolution.

Abstract 1433: The Cardioregulatory Peptide Apelin is Preferentially Expressed by Endothelial Cells and Upregulated in Myocardial Disease States

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Ramendra K Kundu ◽  
Ahmad Y Sheikh ◽  
Michael Y Ho ◽  
Hyung J Chun ◽  
Diem T Huynh ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Reporter Gene ◽  
Fold Increase ◽  
Cell Types ◽  
Heart Tissue ◽  
Capillary Endothelium ◽  
Lacz Gene ◽  
Lacz Reporter ◽  
Rt Pcr ◽  
Disease States

Introduction: The APJ receptor and its ligand, apelin, comprise a homeostatic, cardio-regulatory pathway. Although cardiac apelin expression levels are altered in humans with cardiac failure, the cell type responsible for apelin expression and modulation in disease states remains unknown. Hypothesis: Apelin production is restricted to the endothelial compartment and is upregulated in states of cardiovascular stress. Methods: Transgenic apelin-LacZ reporter mice (SVJ background) were created by insertion of the nuclear localizing bacterial LacZ gene immediately downstream of the apelin promoter. Mice (n=12) were randomized to left anterior descending coronary artery (LAD) ligation, thoracic aortic constriction (TAC) or sham groups. Hearts were harvested 3 and 8 weeks post-TAC or LAD ligation, respectively. Localization of apelin expression was determined by Xgal staining. Endothelial phenotype of lacZ positive cells was confirmed by CD31 co-staining. Apelin expressing cells were quantified by histology. Apelin reporter results were confirmed by quantitating apelin expression in WT animals following LAD ligation (n=11) or sham (n=11) procedure by RT-PCR. Results: Extensive immunohistochemistry studies of heart tissue revealed lacZ reporter gene expression to be restricted to the coronary venous and capillary endothelium, with no expression by cardiomyocytes. Following both LAD ligation and TAC, the number of LacZ-apelin (+) endothelial cells significantly increased (p<0.002) in all chambers of the heart (Table ), with no evidence of apelin expression by other cell types. Evaluation of WT hearts by RT-PCR for the apelin gene confirmed the reporter gene findings with 1.3±0.3 fold increase (p<0.05) of apelin expression induced by LAD ligation compared to sham. Conclusions: Apelin is primary expressed by endothelial cells within the heart and is upregulated in response to myocardial stress. Apelin-LacZ Expressing Endothelial Cells are Increased Following Myocardial Injury

scholarly journals Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae.

1996 ◽  
Vol 16 (5) ◽  
pp. 2226-2237 ◽  
Author(s):  
K W Cunningham ◽  
G R Fink
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Phosphatase ◽  
Reporter Genes ◽  
Immunosuppressive Drug ◽  
Ion Pumps ◽  
Ion Transporters ◽  
Lacz Reporter ◽  
Dependent Protein ◽  
P Type ◽  
Calcineurin Activity

The PMC1 gene in Saccharomyces cerevisiae encodes a vacuolar Ca2+ ATPase required for growth in high-Ca2+ conditions. Previous work showed that Ca2+ tolerance can be restored to pmc1 mutants by inactivation of calcineurin, a Ca2+/calmodulin-dependent protein phosphatase sensitive to the immunosuppressive drug FK506. We now report that calcineurin decreases Ca2+ tolerance of pmc1 mutants by inhibiting the function of VCX1, which encodes a vacuolar H+/Ca2+ exchanger related to vertebrate Na+/Ca2+ exchangers. The contribution of VCX1 in Ca2+ tolerance is low in strains with a functional calcineurin and is high in strains which lack calcineurin activity. In contrast, the contribution of PMC1 to Ca2+ tolerance is augmented by calcineurin activation. Consistent with these positive and negative roles of calcineurin, expression of a vcx1::lacZ reporter was slightly diminished and a pmc1::lacZ reporter was induced up to 500-fold by processes dependent on calcineurin, calmodulin, and Ca2+. It is likely that calcineurin inhibits VCX1 function mainly by posttranslational mechanisms. Activities of VCX1 and PMC1 help to control cytosolic free Ca2+ concentrations because their function can decrease pmc1::lacZ induction by calcineurin. Additional studies with reporter genes and mutants indicate that PMR1 and PMR2A, encoding P-type ion pumps required for Mn2+ and Na+ tolerance, may also be induced physiologically in response to high-Mn2+ and -Na+ conditions through calcineurin-dependent mechanisms. In these situations, inhibition of VCX1 function may be important for the production of Ca2+ signals. We propose that elevated cytosolic free Ca2+ concentrations, calmodulin, and calcineurin regulate at least four ion transporters in S. cerevisiae in response to several environmental conditions.

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