scholarly journals Differences in relative DNA content between human peripheral blood and bone marrow subpopulations-consequences for DNA index calculations

Cytometry ◽  
1993 ◽  
Vol 14 (8) ◽  
pp. 936-942 ◽  
Author(s):  
Inger Marie Jensen ◽  
Jørgen Ellegaard ◽  
Peter Hokland
Keyword(s):  
Bone Marrow ◽  
Dna Content ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Dna Index ◽  
Relative Dna Content

Flow Cytometrically Determined DNA Content of Breast Carcinoma and Benign Lesions: Correlations with Histopathological Parameters

1986 ◽  
Vol 72 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Raffaella Uccelli ◽  
Alberto Calugi ◽  
Donato Forte ◽  
Francesco Mauro ◽  
Paolo Polonio-Balbi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cell Subpopulation ◽  
Aneuploid Cell ◽  
Breast Carcinomas ◽  
Benign Lesions ◽  
Dna Index ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Relative Dna Content

The relative DNA content of cellular samples from 54 patients affected by breast carcinomas and 20 affected by benign breast lesions (including 11 fibroadenomas) was measured by flow cytometry. All normal tissue samples and 17/20 (85%) specimens from benign lesions exhibited a cytometrically diploid DNA distribution, 3/20 (15%) benign lesions an abnormal DNA content, and 35/54 (65%) carcinomas at least one aneuploid cell subpopulation. Furthermore, 9/54 (17%) tumors were characterized by the presence of more than one aneuploid cell subpopulation. The results also indicate that flow cytometry can be used to recognize lymph nodes infiltrated by aneuploid cells. Statistically significant correlations were evidenced between the occurrence of aneuploidy or the ploidy level measured as DNA index and the nodal infiltration status. The percentage of S cells can also be extracted from DNA content distribution histograms. Statistically significant differences (p < 0.01) were also observed for the percentage of S cells between normal tissues (6.2±3.2 SD) and benign lesions (11.1±6.6 SD), normal tissues (6.2 ± 3.2 SD) and aneuploid tumors (19.7 ± 10.3 SD), benign lesions (11.1 ± 6.6 SD) and aneuploid tumors (19.7 ± 10.3 SD), and diploid (7.9 ± 4.0 SD) and aneuploid tumors (19.7 ± 10.3 SD).

Ranitidine Fails to Suppress the Growth in vitro of Haemopoietic Progenitors from Human Peripheral Blood or Bone Marrow

1989 ◽  
Vol 8 (1) ◽  
pp. 19-22
Author(s):  
C.D.L. Reid ◽  
A. Kirk
Keyword(s):  
Bone Marrow ◽  
Drug Concentration ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Erythroid Progenitors ◽  
Drug Concentrations ◽  
Normal Human ◽  
Normal Human Peripheral Blood ◽  
Clonal Assays

Ranitidine was added in various concentrations (25-1600 ng/ml) to clonal assays of haemopoietic progenitors of normal human peripheral blood or bone marrow. Although a significant reduction in colonies forming from granulocyte-macrophage progenitors (CFU-GM) was demonstrated at the lowest drug concentration, no significant growth suppression was seen at higher concentrations. There was no evidence for growth inhibition of either erythroid progenitors (BFU-E) or pluripotent progenitors (CFU-mix) at any of the drug concentrations studied. A direct toxic effect of ranitidine on normal haemopoietic progenitors thus appears an unlikely cause of cytopenias observed during treatment.

scholarly journals Cytokine-Activated Endothelium Recruits Osteoclast Precursors

Endocrinology ◽  
2001 ◽  
Vol 142 (4) ◽  
pp. 1678-1681 ◽  
Author(s):  
Neil W. A. McGowan ◽  
Emily J. Walker ◽  
Heather Macpherson ◽  
Stuart H. Ralston ◽  
Miep H. Helfrich
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Bone Resorption ◽  
Vitamin D3 ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Osteoclast Formation ◽  
Bone Surface ◽  
Osteoclast Precursors

Abstract Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recruitment to sites of bone resorption. Osteoclast precursors in human peripheral blood were identified by their ability to form mature osteoclasts in 21-day cultures supplemented with RANKLigand, M-CSF, 1,25(OH)2-vitamin D3, dexamethasone and prostaglandin E2. Under control conditions few osteoclast precursors adhered to endothelial cells (the human bone marrow-derived endothelial cell line BMEC-1). However, BMEC-1 cells treated with the resorption stimulating cytokines IL-1β and TNFα depleted the PBMC population of all osteoclast precursors. These results provide the first evidence that osteoclast precursors can adhere to endothelium and suggest that endothelium could play an important role in the recruitment of osteoclast precursors to sites of bone resorption.

scholarly journals Identification of pure and mixed basophil colonies in culture of human peripheral blood and marrow cells

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 78-83 ◽  
Author(s):  
AG Leary ◽  
M Ogawa
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Human Peripheral Blood ◽  
Morphological Characteristics ◽  
Assay System ◽  
Immunofluorescent Staining ◽  
Colony Assay ◽  
Marrow Cells

Abstract We present a colony assay system that allows in situ identification of human basophil/mast cell (basophil) colonies. In methylcellulose culture, in the presence of phytohemagglutinin-leukocyte conditioned media (PHA-LCM), human peripheral blood and bone marrow cells form colonies that can be distinguished by their unique morphological characteristics. Pure basophil colonies are diffuse, small colonies containing small, round, highly refractile cells. These characteristics of the constituent cells led us to the observation that a significant number of basophils are found in combination with eosinophils. The mixed eosinophil/basophil colonies have the distinctive elements of pure eosinophil and pure basophil colonies. Usually, these are diffuse colonies with compact clusters of slightly larger, darker-appearing cells. We also found colonies that contained basophils and neutrophils/monocytes, but this type could not be consistently identified by in situ morphology. Cytochemical analysis confirmed the metachromatic nature of the granules in the basophils. The presence of IgE receptors on the cells was documented by indirect immunofluorescent staining after passive sensitization with purified human IgE. Peripheral blood cells from six healthy volunteers formed 5.7 +/- 1.0 (mean +/- SEM) pure colonies in 2 X 10(5) cells. Cultures of bone marrow cells from patients with various types of anemia had 9.0 +/- 1.5 colonies in 10(5) cells. This is the first description of a colony assay system for in situ identification of a pure population of basophilic granulocytes.

Detection of BLT substrate-specific proteases in individual human peripheral blood leucocytes and bone marrow cells

1991 ◽  
Vol 142 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Ludwig Wagner ◽  
Woflgang Base ◽  
Martin Wiesholzer ◽  
Veronika Sexl ◽  
Hartwig Bognar ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leucocytes ◽  
Individual Human ◽  
Marrow Cells

Engraftment of human peripheral blood lymphocytes in normal strains of mice

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2368-2381 ◽  
Author(s):  
I Lubin ◽  
H Segall ◽  
H Marcus ◽  
M David ◽  
L Kulova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Peripheral Blood ◽  
Scid Mouse ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Internal Organs ◽  
Human Peripheral Blood Lymphocytes ◽  
Hla Dr ◽  
Human Pbls

Transplantation of bone marrow from SCID mice into lethally irradiated normal mice can potentially endow the normal recipients with characteristics typical of the immune-deficient SCID mouse. In the present study, we investigated whether intraperitoneal grafting of human peripheral blood lymphocytes (PBLs), which has been documented in the SCID mouse, can also be achieved in irradiated BALB/c mice radioprotected with SCID bone marrow. Evaluation of different radiation protocols suggested that, considering the quality of engraftment and rate of survival, optimal results were obtained with split dose total body irradiation (TBI; 4 Gy followed 3 days later by 10 Gy). Monitoring of mouse T cells in peripheral blood indicated an inverse correlation between the presence of such cells and the engraftment of human CD45+ cells in the peritoneum. Also, engraftment of human PBLs in nude BALB/c mice, conditioned with the same radiation protocol, was significantly higher than that achieved in their normal counterparts. Further improvement of human PBL engraftment was found when the mice were thymectomized 2 weeks before conditioning with split TBI. After transplantation of 80 x 10(6) human PBLs in such recipients, a marked engraftment of human T cells and B cells in the peritoneum cavity could be detected for at least 2 months, whereas significant amounts of human Ig could be detected for more than 3 months. Migration of human PBLs into internal organs such as spleen, liver, kidney, and lungs (and into thymus in nonthymectomized mice) was found within a few days of grafting and also persisted for 2 to 3 months. The majority of the engrafted lymphocytes were single-positive CD4+ and CD8+ T lymphocytes, about 50% of which were activated, as judged by their expression of HLA- DR. Staining with anti-CD25 antibody was lower compared with that found with anti-HLA-DR. CD20+ B cells were detected in all of the above- mentioned internal organs, but were mainly concentrated in the spleen. CD14+ monocytes could be detected only during the first week posttransplant of PBLs. Total human Ig in peripheral blood reached an average of 2.8 mg/mL 14 days posttransplant, and continued to be significant for several months. In vitro transformation by Epstein-Barr virus of human B cells from different tissues could be established 30 days after transplantation and led to outgrowth of two IgG+ cell lines, two IgM+ cell lines, and one IgA+ cell line producing 0.6 to 4.2 micrograms/mL human Ig in the supernatant.

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