Differential regulation of lymphokine production in mitogen-stimulated murine spleen cells

1991 ◽  
Vol 21 (8) ◽  
pp. 1887-1892 ◽  
Author(s):  
Birgitta Sander ◽  
Susanna Cardell ◽  
Göran Möller ◽  
Erna Möller
Keyword(s):  
Differential Regulation ◽  
Spleen Cells ◽  
Lymphokine Production ◽  
Murine Spleen

Surface immunoglobulin phenotype of murine spleen cells which form B cell colonies in agar

1978 ◽  
Vol 8 (4) ◽  
pp. 286-288 ◽  
Author(s):  
D. W. Scott ◽  
Judith E. Layton ◽  
G. R. Johnson
Keyword(s):  
B Cell ◽  
Spleen Cells ◽  
Surface Immunoglobulin ◽  
Murine Spleen

Discrimination between NK and LAK cytotoxic activities of murine spleen cells by MTT assay: differential inhibition by PGE2 and EDTA

2000 ◽  
Vol 241 (1-2) ◽  
pp. 121-129 ◽  
Author(s):  
Fátima Ribeiro-Dias ◽  
José Alexandre Marzagão Barbuto ◽  
Maristela Tsujita ◽  
Sonia Jancar
Keyword(s):  
Mtt Assay ◽  
Cytotoxic Activities ◽  
Differential Inhibition ◽  
Spleen Cells ◽  
Murine Spleen

scholarly journals Augmentation of Killer Activity of Murine Spleen Cells against Allogeneic and Syngeneic Tumor Cells by Inoculation of Alloantigenin vivo

1987 ◽  
Vol 31 (12) ◽  
pp. 1245-1254 ◽  
Author(s):  
Yasuaki Nishi ◽  
Tomohide Hosokawa ◽  
Akira Aoike ◽  
Takeshi Kamahora ◽  
Hiromasa Sudo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Spleen Cells ◽  
Killer Activity ◽  
Syngeneic Tumor ◽  
Murine Spleen

Inhibition of purine nucleoside phosphorylase and mitogen-stimulated transformation in immunocompetent murine spleen cells by formycin B

Life Sciences ◽  
1979 ◽  
Vol 25 (14) ◽  
pp. 1215-1222 ◽  
Author(s):  
Joan Willemot ◽  
Ronald Martineau ◽  
Christine DesRosiers ◽  
Susan Kelly ◽  
Jean Létourneau ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Spleen Cells ◽  
Nucleoside Phosphorylase ◽  
Murine Spleen

scholarly journals Identification of soluble Fc receptors in mouse serum and the conditioned medium of stimulated B cells.

1984 ◽  
Vol 160 (6) ◽  
pp. 1836-1849 ◽  
Author(s):  
E Pure ◽  
C J Durie ◽  
C K Summerill ◽  
J C Unkeless
Keyword(s):  
B Cells ◽  
Normal Mouse ◽  
Culture Medium ◽  
Fold Increase ◽  
Mouse Serum ◽  
Spleen Cells ◽  
Germ Free ◽  
Murine Spleen ◽  
Soluble Molecule ◽  
Lps Activation

We have evaluated the expression of surface Fc gamma 2b/gamma 1R by lipopolysaccharide (LPS)-activated murine spleen cells, the release of soluble Fc gamma 2b/gamma 1R by activated spleen cells, and the presence of circulating Fc gamma 2b/gamma 1R in mouse serum. LPS activation of murine spleen cells and a cloned B cell line, BCL-1 CW 13.20-3B3, resulted in a 5-10-fold increase in surface Fc gamma 2b/gamma 1R and the concominant appearance in the culture medium of a soluble molecule that is antigenically related to the Fc gamma 2b/gamma 1R. The increase in cell-associated and soluble Fc gamma 2b/gamma 1R after LPS activation is attributable primarily to B cells. Circulating Fc gamma 2b/gamma 1R was also detected in normal mouse serum at a concentration of 10(-9) to 10(-8) M. Levels of circulating Fc gamma 2b/gamma 1R increase with the age of the animals, and were low in adult germ-free mice and elevated in young mice with certain autoimmune diseases. The circulating Fc gamma 2b/gamma 1R bound to IgG-Sepharose, and was partially purified by affinity chromatography on 2.4G2 Fab-Sepharose. After radiolabeling and immunoprecipitation with rabbit anti-Fc gamma 2b/gamma 1R serum, one component of Mr 48,000, was detected.

scholarly journals Differential regulation of spleen cell-mediated eosinophil and neutrophil-macrophage production

Blood ◽  
1980 ◽  
Vol 55 (3) ◽  
pp. 489-493 ◽  
Author(s):  
SH Bartelmez ◽  
WH Dodge ◽  
DA Bass
Keyword(s):  
Growth Factor ◽  
Contrast Media ◽  
Spleen Cell ◽  
Trichinella Spiralis ◽  
Cell Types ◽  
Differential Regulation ◽  
Spleen Cells ◽  
Secretory Products ◽  
Kinetics Of

Abstract Nonadherent spleen cells of mice infected with Trichinella spiralis released growth stimulatory factors (GSFs) in vitro when challenged with excretory/secretory products of muscle stage larvae. The assay of GSF was based on proliferation of normal, nonadherent syngeneic marrow cells in liquid tube cultures. Media conditioned for 1 day by challenged spleen cells stimulated eosinophil production but failed to stimulate production of other cell types. In contrast, media conditioned for 5 days supported eosinophil, neutrophil, and macrophage production. The kinetics of cell production were also different. Eosinophil production started within 1 day, reached a peak at day 2, and was down to control levels by day 4. In contrast, neutrophil/macrophage production began between 2 and 4 days and reached a peak at 6--8 days. The short duration of eosinophil production was evidently due to depletion of growth-factor-responsive cells.

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