Discrimination between NK and LAK cytotoxic activities of murine spleen cells by MTT assay: differential inhibition by PGE2 and EDTA

2000 ◽  
Vol 241 (1-2) ◽  
pp. 121-129 ◽  
Author(s):  
Fátima Ribeiro-Dias ◽  
José Alexandre Marzagão Barbuto ◽  
Maristela Tsujita ◽  
Sonia Jancar
Keyword(s):  
Mtt Assay ◽  
Cytotoxic Activities ◽  
Differential Inhibition ◽  
Spleen Cells ◽  
Murine Spleen

Rhodium(i) N-heterocyclic carbene complexes: synthesis and cytotoxic properties

2021 ◽  
Vol 45 (11) ◽  
pp. 5176-5183
Author(s):  
Ichraf Slimani ◽  
Serap Şahin-Bölükbaşı ◽  
Mustafa Ulu ◽  
Enes Evren ◽  
Nevin Gürbüz ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mtt Assay ◽  
Incubation Time ◽  
Human Cancer ◽  
Cytotoxic Activities ◽  
Carbene Complexes ◽  
Human Cancer Cells ◽  
Benzimidazolium Salts ◽  
Ht 29

A series of benzimidazolium salts and their [RhCl(NHC)(COD)] complexes were synthesized. All compounds were screened for in vitro cytotoxic activities against a panel of human cancer cells (HT-29 colon, Ishikawa endometrial, U-87 glioblastoma) using the MTT assay for 48 h incubation time.

Surface immunoglobulin phenotype of murine spleen cells which form B cell colonies in agar

1978 ◽  
Vol 8 (4) ◽  
pp. 286-288 ◽  
Author(s):  
D. W. Scott ◽  
Judith E. Layton ◽  
G. R. Johnson
Keyword(s):  
B Cell ◽  
Spleen Cells ◽  
Surface Immunoglobulin ◽  
Murine Spleen

scholarly journals Augmentation of Killer Activity of Murine Spleen Cells against Allogeneic and Syngeneic Tumor Cells by Inoculation of Alloantigenin vivo

1987 ◽  
Vol 31 (12) ◽  
pp. 1245-1254 ◽  
Author(s):  
Yasuaki Nishi ◽  
Tomohide Hosokawa ◽  
Akira Aoike ◽  
Takeshi Kamahora ◽  
Hiromasa Sudo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Spleen Cells ◽  
Killer Activity ◽  
Syngeneic Tumor ◽  
Murine Spleen

Inhibition of purine nucleoside phosphorylase and mitogen-stimulated transformation in immunocompetent murine spleen cells by formycin B

Life Sciences ◽  
1979 ◽  
Vol 25 (14) ◽  
pp. 1215-1222 ◽  
Author(s):  
Joan Willemot ◽  
Ronald Martineau ◽  
Christine DesRosiers ◽  
Susan Kelly ◽  
Jean Létourneau ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Spleen Cells ◽  
Nucleoside Phosphorylase ◽  
Murine Spleen

Differential regulation of lymphokine production in mitogen-stimulated murine spleen cells

1991 ◽  
Vol 21 (8) ◽  
pp. 1887-1892 ◽  
Author(s):  
Birgitta Sander ◽  
Susanna Cardell ◽  
Göran Möller ◽  
Erna Möller
Keyword(s):  
Differential Regulation ◽  
Spleen Cells ◽  
Lymphokine Production ◽  
Murine Spleen

scholarly journals Identification of soluble Fc receptors in mouse serum and the conditioned medium of stimulated B cells.

1984 ◽  
Vol 160 (6) ◽  
pp. 1836-1849 ◽  
Author(s):  
E Pure ◽  
C J Durie ◽  
C K Summerill ◽  
J C Unkeless
Keyword(s):  
B Cells ◽  
Normal Mouse ◽  
Culture Medium ◽  
Fold Increase ◽  
Mouse Serum ◽  
Spleen Cells ◽  
Germ Free ◽  
Murine Spleen ◽  
Soluble Molecule ◽  
Lps Activation

We have evaluated the expression of surface Fc gamma 2b/gamma 1R by lipopolysaccharide (LPS)-activated murine spleen cells, the release of soluble Fc gamma 2b/gamma 1R by activated spleen cells, and the presence of circulating Fc gamma 2b/gamma 1R in mouse serum. LPS activation of murine spleen cells and a cloned B cell line, BCL-1 CW 13.20-3B3, resulted in a 5-10-fold increase in surface Fc gamma 2b/gamma 1R and the concominant appearance in the culture medium of a soluble molecule that is antigenically related to the Fc gamma 2b/gamma 1R. The increase in cell-associated and soluble Fc gamma 2b/gamma 1R after LPS activation is attributable primarily to B cells. Circulating Fc gamma 2b/gamma 1R was also detected in normal mouse serum at a concentration of 10(-9) to 10(-8) M. Levels of circulating Fc gamma 2b/gamma 1R increase with the age of the animals, and were low in adult germ-free mice and elevated in young mice with certain autoimmune diseases. The circulating Fc gamma 2b/gamma 1R bound to IgG-Sepharose, and was partially purified by affinity chromatography on 2.4G2 Fab-Sepharose. After radiolabeling and immunoprecipitation with rabbit anti-Fc gamma 2b/gamma 1R serum, one component of Mr 48,000, was detected.

scholarly journals Decorosides A and B, Cytotoxic Flavonoid Glycosides from the Leaves of Rhododendron decorum

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Mostafa E. Rateb ◽  
Hossam M. Hassan ◽  
El-Shaimaa A. Arafa ◽  
Marcel Jaspars ◽  
Rainer Ebel
Keyword(s):  
Spectral Data ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Methanolic Extract ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
Flavonoid Glycosides ◽  
Cytotoxic Activities ◽  
Diphenyltetrazolium Bromide

Bioassay and NMR-guided fractionation of the methanolic extract of Rhododendron decorum leaves resulted in the isolation of two new flavonoid glycosides, 5,7-dihydroxy-6,8-dimethyldihydroflavanone-7-O-α-L-arabinopyranosyl(l→6)-β-D-glucopyranoside (decoroside A, 1) and its 3-hydroxy congener (decoroside B, 2), along with five known compounds myricitrin (3), afzelin (4), (-)-epicatechin (5), (+)-catechin (6), and ampeloptin (7). The structures of the isolated compounds were elucidated by extensive interpretation of their spectral data. Biological evaluation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed promising cytotoxic activities of these compounds against different cancer cell lines.

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