Recent developments in CE-based detection methods for food-borne pathogens

The vital importance of rapid and accurate detection of food borne pathogens has driven the development of biosensor to prevent food borne illness outbreaks. Electrochemical DNA biosensors offer such merits as rapid response, high sensitivity, low cost, and ease of use. This review covers the following three aspects: food borne pathogens and conventional detection methods, the design and fabrication of electrochemical DNA biosensors and several techniques for improving sensitivity of biosensors. We highlight the main bioreceptors and immobilizing methods on sensing interface, electrochemical techniques, electrochemical indicators, nanotechnology, and nucleic acid-based amplification. Finally, in view of the existing shortcomings of electrochemical DNA biosensors in the field of food borne pathogen detection, we also predict and prospect future research focuses from the following five aspects: specific bioreceptors (improving specificity), nanomaterials (enhancing sensitivity), microfluidic chip technology (realizing automate operation), paper-based biosensors (reducing detection cost), and smartphones or other mobile devices (simplifying signal reading devices).

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Yersinia enterocoliticaandYersinia pseudotuberculosisDetection in Foods

Journal of Pathogens ◽

10.4061/2011/735308 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 19

Author(s):

H. Fukushima ◽

S. Shimizu ◽

Y. Inatsu

Keyword(s):

Food Safety ◽

Detection Methods ◽

Culture Methods ◽

Selective Media ◽

Conventional Culture ◽

Food Borne Pathogens ◽

Food Borne ◽

Isolation Methods ◽

Pcr Assays ◽

Virulence Properties

Yersinia enterocoliticaandY. pseudotuberculosiswhich can cause yersiniosis in humans and animals are thought to be significant food-borne pathogens and be important as hygiene indicator in food safety. The pathogenicY. enterocoliticaserotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 27/2, O:8/1b, and O:9/2, have been reported worldwide.Y. pseudotuberculosisis distributed less widely thanY. enterocolitica. Isolation methods usually involve selective and recovery enrichment of the food sample followed by plating onto selective media, confirmation of typical colonies and testing for virulence properties of isolated strains. Recently, DNA-based methods, such as PCR assays, have been developed to detect pathogenicY. enterocoliticaandY. pseudotuberculosisin foods more rapidly, and sensitivity than can be achieved by conventional culture methods. This paper reviews commercially available conventional and PCR-based procedures for the detection of pathogenicYersiniain food. These methods are effective as the isolation and detection methods to target pathogenicY. enterocoliticaandY. pseudotuberculosisin foods.

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Quantum dot biosensor combined with antibody and aptamer for tracing food-borne pathogens

Food Quality and Safety ◽

10.1093/fqsafe/fyab019 ◽

2021 ◽

Vol 5 ◽

Author(s):

Feifei Sun ◽

Jing Zhang ◽

Qingli Yang ◽

Wei Wu

Keyword(s):

Quantum Dots ◽

Pathogen Detection ◽

High Sensitivity ◽

High Specificity ◽

Detection Methods ◽

Food Borne Pathogens ◽

Food Borne ◽

Recognition Elements ◽

Different Characteristics ◽

And Control

Abstract Due to the increasing number of food-borne diseases, more attention is being paid to food safety. Food-borne pathogens are the main cause of food-borne diseases, which seriously endanger human health, so it is necessary to detect and control them. Traditional detection methods cannot meet the requirements of rapid detection of food due to many shortcomings, such as being time-consuming, laborious or requiring expensive instrumentation. Quantum dots have become a promising nanotechnology in pathogens tracking and detection because of their excellent optical properties. New biosensor detection methods based on quantum dots are have been gradually developed due to their high sensitivity and high specificity. In this review, we summarize the different characteristics of quantum dots synthesized by carbon, heavy metals and composite materials firstly. Then, attention is paid to the principles, advantages and limitations of the quantum dots biosensor with antibodies and aptamers as recognition elements for recognition and capture of food-borne pathogens. Finally, the great potential of quantum dots in pathogen detection is summarized.

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Factors Involved in the Emergence and Persistence of Food-Borne Diseases†

Journal of Food Protection ◽

10.4315/0362-028x-58.6.696 ◽

1995 ◽

Vol 58 (6) ◽

pp. 696-708 ◽

Cited By ~ 27

Author(s):

JAMES L. SMITH ◽

PINA M. FRATAMICO

Keyword(s):

Distribution Systems ◽

Pathogen Detection ◽

Eating Habits ◽

Disease Outbreaks ◽

Detection Methods ◽

Genetic Changes ◽

Food Borne Pathogens ◽

Food Borne ◽

Production And Distribution ◽

Health Significance

In recent years, a number of bacteria, viruses, and parasites have emerged as food-borne pathogens and resulted in numerous food-borne disease outbreaks. These outbreaks have had a major impact in terms of loss of human lives and economic costs. Genetic changes in microorganisms resulting in increased virulence, changes in social attitudes and eating habits, changes in food production and distribution systems, an increase in the number of immunocompromised individuals, and improved pathogen-detection methods are some of the factors that have contributed to the emergence/recognition and persistence of food-borne pathogens. The causes leading to the emergence of new food-borne pathogens or the reemergence of pathogens involve the interaction of several factors. This review discusses in detail factors involved in the emergence/recognition and persistence of several bacterial, parasitic, viral, and virus-like agents associated with food-borne diseases of public-health significance.

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